Live imaging of adult neural stem cell behavior in the intact and injured zebrafish brain

Barbosa, Joana S.; Sánchez-González, Rosario; Giaimo, Rossella Di; Baumgart, Emily Violette; Theis, Fabian J.; Götz, Magdalena; Ninkovic, Jovica

doi:10.1126/science.aaa2729

Cited by 170 publications

(260 citation statements)

References 22 publications

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“…The implication of heterogeneous brain stem cells for tissue regeneration is not well understood. However, the limited availability or depletion of specific stem and progenitor cells could impair the regeneration of specific brain cell lineages (Liu et al, 2009;Encinas et al, 2011;Barbosa et al, 2015). Teleost fish have a much greater ability to regenerate CNS injuries than mammals (Becker et al, 1997;Kaslin et al, 2008;Reimer et al, 2008;Kroehne et al, 2011;Baumgart et al, 2012;Kishimoto et al, 2012;Kizil et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Distinct roles of neuroepithelial-like and radial glia-like progenitor cells in cerebellar regeneration

et al. 2017

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Zebrafish can regenerate after brain injury, and the regenerative process is driven by resident stem cells. Stem cells are heterogeneous in the vertebrate brain, but the significance of having heterogeneous stem cells in regeneration is not understood. Limited availability of specific stem cells might impair the regeneration of particular cell lineages. We studied regeneration of the adult zebrafish cerebellum, which contains two major stem and progenitor cell types: ventricular zone and neuroepithelial cells. Using conditional lineage tracing we demonstrate that cerebellar regeneration depends on the availability of specific stem cells. Radial glia-like cells are thought to be the predominant stem cell type in homeostasis and after injury. However, we find that radial glia-like cells play a minor role in adult cerebellar neurogenesis and in recovery after injury. Instead, we find that neuroepithelial cells are the predominant stem cell type supporting cerebellar regeneration after injury. Zebrafish are able to regenerate many, but not all, cell types in the cerebellum, which emphasizes the need to understand the contribution of different adult neural stem and progenitor cell subtypes in the vertebrate central nervous system.

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Section: Introductionmentioning

confidence: 99%

Distinct roles of neuroepithelial-like and radial glia-like progenitor cells in cerebellar regeneration

et al. 2017

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“…Zebrafish excel as a model to uncover gene function (59,60) and are now advancing research in many fields, including stem cell biology (61,62), cancer (63,64), infectious disease (65,66), and autoimmune disease studies (67,68). In this context, we consider the identification of expressed genes in our zebrafish MHC assembly to be noteworthy.…”

Section: Discussionmentioning

confidence: 99%

Alternative haplotypes of antigen processing genes in zebrafish diverged early in vertebrate evolution

McConnell

Hernandez

Wcisel

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

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Antigen processing and presentation genes found within the MHC are among the most highly polymorphic genes of vertebrate genomes, providing populations with diverse immune responses to a wide array of pathogens. Here, we describe transcriptome, exome, and whole-genome sequencing of clonal zebrafish, uncovering the most extensive diversity within the antigen processing and presentation genes of any species yet examined. Our CG2 clonal zebrafish assembly provides genomic context within a remarkably divergent haplotype of the core MHC region on chromosome 19 for six expressed genes not found in the zebrafish reference genome: mhc1uga, proteasome-β 9b (psmb9b), psmb8f, and previously unknown genes psmb13b, tap2d, and tap2e. We identify ancient lineages for Psmb13 within a proteasome branch previously thought to be monomorphic and provide evidence of substantial lineage diversity within each of three major trifurcations of catalytic-type proteasome subunits in vertebrates: Psmb5/Psmb8/Psmb11, Psmb6/ Psmb9/Psmb12, and Psmb7/Psmb10/Psmb13. Strikingly, nearby tap2 and MHC class I genes also retain ancient sequence lineages, indicating that alternative lineages may have been preserved throughout the entire MHC pathway since early diversification of the adaptive immune system ∼500 Mya. Furthermore, polymorphisms within the three MHC pathway steps (antigen cleavage, transport, and presentation) are each predicted to alter peptide specificity. Lastly, comparative analysis shows that antigen processing gene diversity is far more extensive than previously realized (with ancient coelacanth psmb8 lineages, shark psmb13, and tap2t and psmb10 outside the teleost MHC), implying distinct immune functions and conserved roles in shaping MHC pathway evolution throughout vertebrates.comparative genomics | proteasome and TAP evolution | major histocompatibility | MHC class I pathway | CG2 clonal zebrafish

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“…Direct differentiation of stem cells was recently described and is accompanied by a decrease of the NSC pool [38]. Accordingly, there is a decreasing trend for proliferating Nestinpositive cells in LR-NP+60s-treated cells that, if derived from direct conversion, might contribute to the statistical difference at 7 days after treatment.…”

Section: Discussionmentioning

confidence: 97%

Blue light potentiates neurogenesis induced by retinoic acid-loaded responsive nanoparticles

et al. 2017

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Live imaging of adult neural stem cell behavior in the intact and injured zebrafish brain

Cited by 170 publications

References 22 publications

Distinct roles of neuroepithelial-like and radial glia-like progenitor cells in cerebellar regeneration

Distinct roles of neuroepithelial-like and radial glia-like progenitor cells in cerebellar regeneration

Alternative haplotypes of antigen processing genes in zebrafish diverged early in vertebrate evolution

Blue light potentiates neurogenesis induced by retinoic acid-loaded responsive nanoparticles

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