2019
DOI: 10.1007/978-1-4939-9492-2_9
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Live Imaging of ESCRT Proteins in Microfluidically Isolated Hippocampal Axons

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Cited by 5 publications
(8 citation statements)
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“…We next performed imaging experiments in tripartite microfluidic chambers that isolate cell bodies from axons while enabling synapse formation between neurons in adjacent compartments ( Taylor et al, 2005 ; Birdsall et al, 2019 ) ( Fig S2A ). Lentiviral transduction of cell bodies in these chambers led to moderate protein expression levels and consistent transduction efficiency across constructs used in this study (∼50%; Fig S2B ).…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…We next performed imaging experiments in tripartite microfluidic chambers that isolate cell bodies from axons while enabling synapse formation between neurons in adjacent compartments ( Taylor et al, 2005 ; Birdsall et al, 2019 ) ( Fig S2A ). Lentiviral transduction of cell bodies in these chambers led to moderate protein expression levels and consistent transduction efficiency across constructs used in this study (∼50%; Fig S2B ).…”
Section: Resultsmentioning
confidence: 99%
“…Neuro2a (N2a) neuroblastoma cells (ATCC CCL-131) and HEK293T cells (Sigma-Aldrich) were cultured in DMEM-GlutaMAX (Thermo Fisher Scientific/Invitrogen) with 10% FBS (Atlanta Biological) and Antibiotic-Antimycotic (Thermo Fisher Scientific/Invitrogen) and kept at 37°C in 5% CO 2 . Microfluidic molds and chambers were prepared as described in Birdsall et al (2019) . Chambers were prepared by pouring ∼30 ml of PDMS mixture (QSil 216) into molds, then baking at 70°C overnight.…”
Section: Methodsmentioning
confidence: 99%
“…Microfluidic molds and chambers were prepared as described in (Birdsall et al, 2019). Chambers were prepared by pouring ∼30mL of PDMS mixture (QSil 216) into molds, then baking at 70° C overnight.…”
Section: Methodsmentioning
confidence: 99%
“…Lentivirus was produced using HEK293T cells as previously described (Birdsall et al, 2019; Sheehan et al, 2016). Neurons were transduced with 50-150 µL of lentivirus on 7 DIV, and imaged or fixed on 13-15 DIV.…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation