Live imaging of postembryonic developmental processes in C. elegans

Li, Tingting; Wang, Xinjian; Feng, Zhi Gang; Zou, Yan

doi:10.1016/j.xpro.2022.101336

Cited by 2 publications

(2 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For detect the subcellular localization of DMA-1 in different mutations, hermaphrodite animals were anesthetized using 5 mM levamisole in M9 buffer (Li et al, 2022; Wang et al, 2021), mounted on 2% agar pads and imaged using Zeiss Cell Observer SD spinning disk confocal microscope equipped with an alpha Plan-Apochromat 63x/1.46 NA objective. Fluorescence signals under excitation light at 488 nm and 561 nm were acquired, respectively (for the image shown in Figures 3A, 5D).…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

ATP13A1 engages GET3 to facilitate substrate-specific translocation

Yang,

Li,

Fang

et al. 2024

Preprint

Self Cite

View full text Add to dashboard Cite

Proper localization of proteins to their final destinations is crucial for preserving cellular structure and functions. The interpretation and sorting of highly variable targeting sequences in secreted and membrane proteins, however, pose a challenge in achieving precise localization within specific secretory apparatus. In this study, we demonstrate that atypical signal sequences characterized by high hydrophobicity and/or the absence of characteristic charges undergo targeting to the endoplasmic reticulum (ER) in a reverse orientation, followed by partial cleavage. The P5A-ATPase ATP13A1 recognizes the cleaved signal sequence and dislocates it to the targeting factor GET3, subsequently engaging SEC61 for further translocation. Our findings unveil a comprehensive translocation pathway that operates in a substrate-specific manner, ensuring both high efficiency and fidelity in the protein subcellular localization.

show abstract

Section: Methodsmentioning

confidence: 99%

“…For detect the subcellular localization of DMA-1 in different mutations, hermaphrodite animals were anesthetized using 5 mM levamisole in M9 buffer (Li et al, 2022;Wang et al, 2021) 3B).…”

Section: Imagingmentioning

confidence: 99%