Live Imaging of the Lung

Looney, Mark R.; Bhattacharya, Jahar

doi:10.1146/annurev-physiol-021113-170331

Cited by 62 publications

(55 citation statements)

References 79 publications

(99 reference statements)

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“…However, unlike peripheral microcirculation, in vivo visualization of endothelial glycocalyx layer in pulmonary microcirculation using erythrocyte exclusion zone imaging was technically challenging due to motion-artifact induced by respiration and difficulty in tracking individual erythrocytes in multiple interconnected capillary segments [46]. Rapid scanning using rotating polygonal mirror which enables real-time video-rate confocal fluorescent imaging to identify individual erythrocytes with negative-contrast could be a valid approach to mitigate difficulties described above, whereas most commercial confocal microscopy with the slower scanning device would suffer severe motion-artifact induced blurring.…”

Section: Discussionmentioning

confidence: 99%

Intravital imaging of a pulmonary endothelial surface layer in a murine sepsis model

Park¹,

Choe²,

Seo³

et al. 2018

Biomed. Opt. Express

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Direct intravital imaging of an endothelial surface layer (ESL) in pulmonary microcirculation could be a valuable approach to investigate the role of a vascular endothelial barrier in various pathological conditions. Despite its importance as a marker of endothelial cell damage and impairment of the vascular system, in vivo visualization of ESL has remained a challenging technical issue. In this work, we implemented a pulmonary microcirculation imaging system integrated to a custom-design video-rate laser scanning confocal microscopy platform. Using the system, a real-time cellular-level microscopic imaging of the lung was successfully performed, which facilitated a clear identification of individual flowing erythrocytes in pulmonary capillaries. Subcellular level pulmonary ESL was identified in vivo by fluorescence angiography using a dextran conjugated fluorophore to label blood plasma and the red blood cell (RBC) exclusion imaging analysis. Degradation of ESL width was directly evaluated in a murine sepsis model in vivo, suggesting an impairment of pulmonary vascular endothelium and endothelial barrier dysfunction. contribution to coronary effects of adenosine," Cardiovasc.

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Section: Discussionmentioning

confidence: 99%

Intravital imaging of a pulmonary endothelial surface layer in a murine sepsis model

Park¹,

Choe²,

Seo³

et al. 2018

Biomed. Opt. Express

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“…Developing better techniques to interrogate neutrophil-endothelial interactions in the lung would seem paramount; the continued development of live imaging in transgenic mice using fluorescent probes to label specific cell types176 and increased application of isolated perfused lung models177 as well as human ex vivo and in vivo models represent mechanisms to maximise the translation of preclinical data into effective therapeutics. It remains to be seen whether the so-called ‘lungs on a chip’,178 which combine biomimetic systems containing microfluidic channels lined by living human cells, will develop into tractable models for lung biology per se or specifically ARDS pathobiology.…”

Section: Future Directionsmentioning

confidence: 99%

The pulmonary endothelium in acute respiratory distress syndrome: insights and therapeutic opportunities

Millar

Summers

Griffiths

et al. 2016

Thorax

189

181

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The pulmonary endothelium is a dynamic, metabolically active layer of squamous endothelial cells ideally placed to mediate key processes involved in lung homoeostasis. Many of these are disrupted in acute respiratory distress syndrome (ARDS), a syndrome with appreciable mortality and no effective pharmacotherapy. In this review, we consider the role of the pulmonary endothelium as a key modulator and orchestrator of ARDS, highlighting advances in our understanding of endothelial pathobiology and their implications for the development of endothelial-targeted therapeutics including cell-based therapies. We also discuss mechanisms to facilitate the translation of preclinical data into effective therapies including the application of biomarkers to phenotype patients with ARDS with a predominance of endothelial injury and emerging biotechnologies that could enhance delivery, discovery and testing of lung endothelial-specific therapeutics.

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“…The second section of this review describes these very techniques, beginning with pioneers such as Malpighi who intellectually filtered his unstable observation before drawing the resulting image, to computerized post-processing strategies based on frame registration of oversampled acquisition through mechanically-stabilized organ. These latter techniques have recently opened a new exciting window upon the dynamic study of the pulmonary immune system [6][7][8][9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Intravital microscopy of the lung: minimizing invasiveness

Fiole

Tournier

2016

Journal of Biophotonics

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In vivo microscopy has recently become a gold standard in lung immunology studies involving small animals, largely benefiting from the democratization of multiphoton microscopy allowing for deep tissue imaging. This technology represents currently our only way of exploring the lungs and inferring what happens in human respiratory medicine. The interest of lung in vivo microscopy essentially relies upon its relevance as a study model, fulfilling physiological requirements in comparison with in vitro and ex vivo experiments. However, strategies developed in order to overcome movements of the thorax caused by breathing and heartbeats remain the chief drawback of the technique and a major source of invasiveness. In this context, minimizing invasiveness is an unavoidable prerequisite for any improvement of lung in vivo microscopy. This review puts into perspective the main techniques enabling lung in vivo microscopy, providing pros and cons regarding invasiveness.

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Live Imaging of the Lung

Cited by 62 publications

References 79 publications

Intravital imaging of a pulmonary endothelial surface layer in a murine sepsis model

Intravital imaging of a pulmonary endothelial surface layer in a murine sepsis model

The pulmonary endothelium in acute respiratory distress syndrome: insights and therapeutic opportunities

Intravital microscopy of the lung: minimizing invasiveness

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