Live Imaging Reveals Differing Roles of Macrophages and Neutrophils during Zebrafish Tail Fin Regeneration

Li, Li; Yan, Bo; Shi, Yuqian; Zhang, Wenqing; Wen, Zilong

doi:10.1074/jbc.m112.349126

Cited by 303 publications

(285 citation statements)

References 47 publications

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“…Under this scab, the granulation tissue started to spread and macrophages appeared to remove large cell debris as well as apoptotic neutrophils. These macrophages are the key scavengers for resolving inflammation and facilitating tissue regeneration (Li et al, 2012). An accentuated inflammatory rim seemed more obvious around the untreated burns than in the treated ones.…”

Section: Discussionmentioning

confidence: 99%

The evaluation of the healing proprieties of pumpkin and linseed oils on deep second-degree burns in rats

Bardaa

Moalla

Khedir

et al. 2015

Pharmaceutical Biology

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(2016) The evaluation of the healing proprieties of pumpkin and linseed oils on deep second-degree burns in rats, Pharmaceutical Biology, 54:4, 581-587, DOI: 10.3109/13880209.2015 Objective: The present study assesses the healing potential of pumpkin and linseed extracted oils on rats. Materials and methods: Uniform deep second-degree burns were induced on the dorsum of 24 rats, randomly divided into four groups. The burns were measured, photographed, and topically treated with saline solution, ''Cytol Centella Õ '', pumpkin, and linseed-extracted oils (0.52 ml/mm 2 of oil) each 2 d (up until day 33). Post-burning of the 33rd day, biopsies were histologically assessed. Results: At the end of the experiment, the rat groups treated with linseed, pumpkin oils, and ''Cytol Centella Õ '' had higher percentage of wound contraction (98.68, 96.71, and 92.54%, respectively) than the control group (58.38%). Wound biopsies from rats treated with extracted oils showed the best tissue regeneration proprieties as compared with the other groups. The histomorphometric analysis of biopsies revealed that linseed oil could significantly stimulate angiogenesis (55.6% ± 7.25). The pumpkin oil, and Cytol Centella Õ could significantly increase the collagen production 64.9% ± 5.94, and 61.2% ± 7.36, respectively. Discussion and conclusion: Overall, our study has given for the first time scientific evidence of the healing efficiency of pumpkin and linseed oils on burn-wounds.

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Section: Discussionmentioning

confidence: 99%

The evaluation of the healing proprieties of pumpkin and linseed oils on deep second-degree burns in rats

Bardaa

Moalla

Khedir

et al. 2015

Pharmaceutical Biology

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“…Similarly, endogenous zDusp6 underwent degradation in zebrafish embryos after H 2 O 2 treatment ( Figure 5C and 5D) signal is required for the rapid inflammatory response in the tail-fin epithelium of zebrafish larvae [25,26]. To differentiate potentially dual function of H 2 O 2 in recruiting inflammatory cells and priming heart regeneration, we examined temporal and spatial distribution of immune cells during heart regeneration by labeling macrophages and neutrophils with either anti-lymphocyte cytosolic protein 1 (Lcp1) or Tg(coro1a:EGFP) [41]. We found that Lcp1 + leukocytes appeared around 3 dpa, peaked at 7 and 14 dpa, decreased at 19 dpa and almost disappeared at 24 dpa ( Figure 6A-6F), and the recruitment occurred in a H 2 O 2 -dependent manner ( Figure 6G and 6I).…”

Section: The Duox-h 2 O 2 -Dusp6 Pathway Primes Myocardial Regeneratimentioning

confidence: 99%

“…Tg(tcf21:nucEGFP) was previously reported [30]. Tg(coro1a:EGFP) was provided by Dr Zilong Wen [41], Tg(dusp6:EGFP) transgenic zebrafish were obtained from Dr Michael Tsang [40] and Tg(gata4:EGFP) transgenic zebrafish were kindly provided by Dr Todd Evans [51]. The zebrafish myl7 promoter was provided by Dr C Geoff Burns [52]; pHyPer-Cyto cDNA clone was purchased from Evrogen (Cat No.…”

Section: Zebrafish Linesmentioning

confidence: 99%

Hydrogen peroxide primes heart regeneration with a derepression mechanism

et al. 2014

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While the adult human heart has very limited regenerative potential, the adult zebrafish heart can fully regenerate after 20% ventricular resection. Although previous reports suggest that developmental signaling pathways such as FGF and PDGF are reused in adult heart regeneration, the underlying intracellular mechanisms remain largely unknown. Here we show that H 2 O 2 acts as a novel epicardial and myocardial signal to prime the heart for regeneration in adult zebrafish. Live imaging of intact hearts revealed highly localized H 2 O 2 (~30 µM) production in the epicardium and adjacent compact myocardium at the resection site. Decreasing H 2 O 2 formation with the Duox inhibitors diphenyleneiodonium (DPI) or apocynin, or scavenging H 2 O 2 by catalase overexpression markedly impaired cardiac regeneration while exogenous H 2 O 2 rescued the inhibitory effects of DPI on cardiac regeneration, indicating that H 2 O 2 is an essential and sufficient signal in this process. Mechanistically, elevated H 2 O 2 destabilized the redox-sensitive phosphatase Dusp6 and hence increased the phosphorylation of Erk1/2. The Dusp6 inhibitor BCI achieved similar pro-regenerative effects while transgenic overexpression of dusp6 impaired cardiac regeneration. H 2 O 2 plays a dual role in recruiting immune cells and promoting heart regeneration through two relatively independent pathways. We conclude that H 2 O 2 potentially generated from Duox/Nox2 promotes heart regeneration in zebrafish by unleashing MAP kinase signaling through a derepression mechanism involving Dusp6.

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“…Delayed regeneration of tail fin after macrophage depletion Zebrafish embryo [20] Macrophages are required for limb regeneration Adult salamander [22] Macrophages phagocytize wound debris and induce fibroblast proliferation during skin repair…”

Section: Macrophage Plasticity and Polarizationmentioning

confidence: 99%

Macrophages – sensors and effectors coordinating skin damage and repair

Willenborg

Eming

2014

J Deutsche Derma Gesell

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SummaryRestoration of skin integrity and homeostasis following injury is a vital process. Wound healing disorders, including chronic skin ulcers and pathological scarring, are of major clinical impact. The current therapeutic approaches are often not sufficient. The development of novel efficient therapies requires a thorough understanding of the underlying molecular mechanisms. A cardinal feature of non-healing skin ulcers and excessive scarring is a prolonged inflammatory response at the wound site, which aborts the healing response. Modulation of the local immune response may be an effective therapeutic strategy to correct impaired healing conditions. Yet, the specific mechanisms of inflammation, particularly the role of the diverse leukocyte lineages attracted to the site of tissue damage, have not been resolved. Recent findings in diverse experimental model systems and clinical studies have refined the understanding of monocyte/macrophage biology and the role of cells of the monocytic lineage in tissue regeneration. Thus, monocytes/macrophages are emerging as novel and interesting therapeutic targets to interfere in wound healing pathologies. In this article we will review the role of monocytes/macrophages in skin repair in the light of the recent literature and findings from our own group. This article will provide a rationale for monocyte/macrophage-based therapies to facilitate the healing response.

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Live Imaging Reveals Differing Roles of Macrophages and Neutrophils during Zebrafish Tail Fin Regeneration

Cited by 303 publications

References 47 publications

The evaluation of the healing proprieties of pumpkin and linseed oils on deep second-degree burns in rats

The evaluation of the healing proprieties of pumpkin and linseed oils on deep second-degree burns in rats

Hydrogen peroxide primes heart regeneration with a derepression mechanism

Macrophages – sensors and effectors coordinating skin damage and repair

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