Liver and kidney cells cultures in a new perfluoropolyether biochip

Jellali, Rachid; Paullier, Patrick; Fleury, Marie-José; Leclerc, Éric

doi:10.1016/j.snb.2016.01.141

Cited by 39 publications

(47 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A) Liver–kidney model: Madin–Darby canine kidney (MDCK) cells cultured in biochip and different parameters including (1) proliferation rate which increased after starting perfusion, (2) cell viability, (3) and glucose consumption were compared. Reproduced with permission . Copyright 2016, Elsevier.…”

Section: Current Organ‐on‐a‐chip Platformsmentioning

confidence: 99%

“…However, they found that HepGA/C3A cells did not metabolized ifosfamide, which indicates the importance of cell type selection and tissue maturation to mimic the human body function. In other multi‐OOC platforms, liver–heart systems were developed and tested . Liver–heart interactions in such platforms could help in analyzing the toxicity and functionality of cardiovascular drugs (Figure A) .…”

Section: Current Organ‐on‐a‐chip Platformsmentioning

confidence: 99%

See 1 more Smart Citation

Organ‐On‐A‐Chip Platforms: A Convergence of Advanced Materials, Cells, and Microscale Technologies

Ahadian

Civitarese

Bannerman

et al. 2017

Adv Healthcare Materials

247

162

View full text Add to dashboard Cite

Significant advances in biomaterials, stem cell biology, and microscale technologies have enabled the fabrication of biologically relevant tissues and organs. Such tissues and organs, referred to as organ-on-a-chip (OOC) platforms, have emerged as a powerful tool in tissue analysis and disease modeling for biological and pharmacological applications. A variety of biomaterials are used in tissue fabrication providing multiple biological, structural, and mechanical cues in the regulation of cell behavior and tissue morphogenesis. Cells derived from humans enable the fabrication of personalized OOC platforms. Microscale technologies are specifically helpful in providing physiological microenvironments for tissues and organs. In this review, biomaterials, cells, and microscale technologies are described as essential components to construct OOC platforms. The latest developments in OOC platforms (e.g., liver, skeletal muscle, cardiac, cancer, lung, skin, bone, and brain) are then discussed as functional tools in simulating human physiology and metabolism. Future perspectives and major challenges in the development of OOC platforms toward accelerating clinical studies of drug discovery are finally highlighted.

show abstract

Section: Current Organ‐on‐a‐chip Platformsmentioning

confidence: 99%

Section: Current Organ‐on‐a‐chip Platformsmentioning

confidence: 99%

Organ‐On‐A‐Chip Platforms: A Convergence of Advanced Materials, Cells, and Microscale Technologies

Ahadian

Civitarese

Bannerman

et al. 2017

Adv Healthcare Materials

247

162

View full text Add to dashboard Cite

show abstract

“…The IDCCM device is the result of the integration of 12 microfluidic biochips into a larger fluidic platform (IDCCM box) allowing the parallelized culture of 12 independent biochips. The concept and detail of the IDCCM box and biochips are described in detail in Supporting information S1 and in our previous work (Baudoin, Alberto, Paullier, Legallais, & Leclerc, ; Baudoin, Griscom, Prot, Legallais, & Leclerc, ; Jellali, Paullier, Fleury, & Leclerc, ).…”

Section: Methodsmentioning

confidence: 99%

“…The analysis was performed using the culture mediums collected at the end of experiments. Albumin synthesis was investigated by an enzyme‐linked immunosorbent assay sandwich technique (anti‐human albumin IgG coupled with peroxidase) following our previous protocol (Baudoin et al, ; Jellali, Paullier, et al, ).…”

Section: Methodsmentioning

confidence: 99%

Metabolomics‐on‐a‐chip approach to study hepatotoxicity of DDT, permethrin and their mixtures

Jellali

Gilard

Pandolfi

et al. 2018

J of Applied Toxicology

Self Cite

View full text Add to dashboard Cite

Despite the diversity of studies on pesticide toxicities, there is a serious lack of information concerning the toxic effect of pesticides mixtures. Dichlorodiphenyl-trichloroethane (DDT) and permethrin (PMT) are among the most prevalent pesticides in the environment and have been the subject of several toxicological studies. However, there are no data on the toxicity of their mixtures. In this study, we used an approach combining cell culture in microfluidic biochips with gas chromatography-mass spectrometry metabolomics profiling to investigate the biomarkers of toxicity of DDT, PMT and their mixtures. All parameters observed indicated that no significant effect was observed in hepatocytes cultures exposed to low doses (15 μm) of DDT and PMT. Conversely, combined low doses induce moderate oxidative stress and cell death. The toxic signature of high doses of pesticides (150 μm) was illustrated by severe oxidative stress and cell mortality. Metabolomics profiling revealed that hepatocytes exposure to DDT150, PMT150 and DDT150 and PMT150 cause important modulation in intermediates of glutathione pathway and tricarboxylic acid cycle, amino acids and metabolites associated to hepatic necrosis and inflammation (α-ketoglutarate, arginine and 2-hydroxybutyrate). These changes were more striking in the combined group. Finally, DDT150 led to a significant increase of benzoate, decanoate, octanoate, palmitate, stearate and tetradecanoate, which illustrates the estrogen modulation. This study demonstrates the potential of metabolomics-on-a-chip approach to improve knowledge on the mode of action of pesticides.

show abstract

“…Glucose consumption and urea production were measured using a biochemical analyzer, the Konelab 20 (Thermo Fisher Electron Corporation, Courtaboeuf,France). The protocols have been described in detail in our previous works (Jellali et al, 2016a(Jellali et al, , 2016b. Briefly, glucose was measured using the glucose oxidase method and a modified Trinder color reaction catalyzed by the enzyme peroxidase GOD-POD Kit (Thermo Electron Corporation).…”

Section: Biological Assaysmentioning

confidence: 99%

Effects of DDT and permethrin on rat hepatocytes cultivated in microfluidic biochips: Metabolomics and gene expression study

Jellali

Zeller

Gilard

et al. 2018

Environmental Toxicology and Pharmacology

Self Cite

View full text Add to dashboard Cite

Dichlorodiphenyl-trichloroethane (DDT) and permethrin (PMT) are amongst most prevalent pesticides in the environment. Although their toxicity has been extensively studied, molecular mechanisms and metabolic effects remain unclear, including in liver where their detoxification occurs. Here, we used metabolomics, coupled to RT-qPCR analysis, to examine effects of DDT and PMT on hepatocytes cultivated in biochips. At 150 μM, DDT caused cell death, cytochrome P450 induction and modulation of estrogen metabolism. Metabolomics analysis showed an increase in some lipids and sugars after 6 h, and a decrease in fatty acids (tetradecanoate, octanoate and linoleate) after 24 h exposure. We also found a change in expression associated with genes involved in hepatic estrogen, lipid, and sugar metabolism. PMT at 150 μM perturbed lipid/sugar homeostasis and estrogen signaling pathway, between 2 and 6 h. After 24 h, lipids and sugars were found to decrease, suggesting continuous energy demand to detoxify PMT. Finally, at 15 μM, DDT and PMT appeared to have a small effect on metabolism and were detoxified after 24 h. Our results show a time-dependent perturbation of sugar/lipid homeostasis by DDT and PMT at 150 μM. Furthermore, DDT at high dose led to cell death, inflammatory response and oxidative stress.

show abstract

Liver and kidney cells cultures in a new perfluoropolyether biochip

Cited by 39 publications

References 61 publications

Organ‐On‐A‐Chip Platforms: A Convergence of Advanced Materials, Cells, and Microscale Technologies

Organ‐On‐A‐Chip Platforms: A Convergence of Advanced Materials, Cells, and Microscale Technologies

Metabolomics‐on‐a‐chip approach to study hepatotoxicity of DDT, permethrin and their mixtures

Effects of DDT and permethrin on rat hepatocytes cultivated in microfluidic biochips: Metabolomics and gene expression study

Contact Info

Product

Resources

About