Liver cell transplantation: The road to clinical application

Allen, Katrina J.; Soriano, Humberto E.

doi:10.1067/mlc.2001.119148

Cited by 67 publications

(42 citation statements)

References 73 publications

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“…Kedelai dan tempe mengandung protein yang tinggi. 9,16 Hal ini sesuai dengan penelitian yang dilakukan oleh Bloom et al bahwa pada kelompok tikus yang diberi diet rendah protein terjadi penurunan status imunologi dan setelah diberi diet tinggi protein terjadi peningkatan status imunologi menjadi seperti kelompok tikus lainnya yang diberi diet tinggi protein sejak awal. 7 Kedelai dan tempe juga mengandung mineralmineral yang berpengaruh terhadap peningkatan sistem imun seperti Zn dan Fe.…”

Section: Pembahasanunclassified

“…7 Kedelai dan tempe juga mengandung mineralmineral yang berpengaruh terhadap peningkatan sistem imun seperti Zn dan Fe. 9,16 Penelitian yang dilakukan oleh Winarsi et al pada kelompok wanita pre menopause yang diberi suplementasi Zn dapat meningkatkan sistem imunnya dibandingkan kelompok kontrol.…”

Section: Pembahasanunclassified

“…16,17 Tempe juga diketahui mengandung vitamin B12 yang juga berperan dalam meningkatkan jumlah leukosit. 9,16 Berdasarkan uraian diatas peneliti tertarik untuk meneliti efek pemberian emulsi kedelai (Glycine max) dan emulsi tempe terhadap hitung limfosit total pada tikus putih (Rattus norvegicus) yang telah ditekan sistem imunnya dengan menggunakan prednison.…”

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Perbandingan Efek Pemberian Emulsi Kedelai dan Emulsi Tempe Terhadap Hitung Limfosit Total pada Tikus Putih yang Telah Diberi Prednison

Nurcahyani¹,

Pardede²,

Masdar³

2017

JIK

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56Lingkungan di sekitar manusia mengandung berbagai jenis patogen yang dapat menyebabkan infeksi misalnya bakteri, virus, jamur, protozoa dan parasit. Infeksi yang terjadi pada orang normal umumnya singkat dan jarang meninggalkan kerusakan permanen. Hal ini disebabkan tubuh manusia memiliki suatu sistem yang disebut sistem imun. Sistem imun terdiri atas sistem imun spesifik dan non-spesifik. Sel limfosit merupakan sel yang berperan utama dalam sistem imun spesifik. 1,2Penurunan atau gagalnya fungsi dari salah satu atau lebih unsur sistem imun disebut imunodefisiensi. Kegagalan fungsi ini sering berkaitan dengan gagalnya kerja limfosit, komplemen dan fagosit.1 Prednison termasuk obat steroid golongan glukokortikoid penyebab imunodefisiensi sekunder.3 Pemberian dosis tunggal glukokortikoid dengan masa kerja pendek akan menurunkan jumlah limfosit dalam sirkulasi. 3,4 Perbandingan Efek Pemberian Emulsi Kedelai dan Emulsi Tempe Terhadap Hitung Limfosit Total pada Tikus Putih yang Telah Diberi PrednisonRizka Bekti Nurcahyani, Imelda T Pardede, Huriatul Masdar * ABSTRACT Adequate nutrition is one of important factors in immunodeficiency repairment. Soybean and tempeh contains proteins, zinc, ferrum, vitamins and isoflavon. Fermentation in tempeh makes it having better nutrients digestion and absorption than soybean. The objective of this study was to compare the effects of soy and tempeh emulsions on total lymphocyte count in rats treated with prednisone. The test was done on 24 male white rats divided into four groups. Group A was given distilled water and group B, C and D had prednisone 2.5 mg/day for 6 days. After that, group A and B were continued having distilled water while groups C or D was fed with soy or tempeh emulsion 0.71 mL/day for 10 days, respectively. The results shown that soy and tempeh emulsion could increase total lymphocyte count significantly (p < 0,05) but there was no significant difference of total lymphocyte count between soy and tempeh emulsion groups (p > 0,05).

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Perbandingan Efek Pemberian Emulsi Kedelai dan Emulsi Tempe Terhadap Hitung Limfosit Total pada Tikus Putih yang Telah Diberi Prednison

Nurcahyani¹,

Pardede²,

Masdar³

2017

JIK

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“…Among children with urea cycle disorders, three of them with ornithine transcarbamylase deficiency (OCT) had NH3 control and evidence of OCT activity on liver biopsy. A 3.5-year-old girl with argininosuccinate lyase (ASL) deficiency and psychomotor retardation received a total of 4.7 × 10 9 hepatocytes (divided into 11 infusions), with important ammonium level reduction, a 3% ASL activity on liver biopsy at 8 mo (undetectable at baseline), and evidence of engrafted male cells (12.5%) at 1 year [2,[7][8][9][10] . A 9-year-old Crigler-Najjar type 1 baby achieved a 50% reduction of bilirubin after receiving 5% of the hepatic mass divided into three intrahepatic infusions over 24 h, and returned toward pre-transplant levels 2 years later, despite evidence of functioning, engrafted allogenic hepatocytes [12] .…”

Section: Adult Ht For Metabolic Liver Diseasesmentioning

confidence: 99%

Clinical applications of hepatocyte transplantation

Pietrosi¹,

Vizzini²,

Gruttadauria³

et al. 2009

WJG

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The shortage of organ donors is a problem worldwide, with approximately 15% of adult patients with lifethreatening liver diseases dying while on the waiting list. The use of cell transplantation for liver disease is an attempt to correct metabolic defects, or to support liver function as a bridge to liver transplantation and, as such, has raised a number of expectations. Most of the available studies briefly reported here focus on adult hepatocyte transplantation (HT), and the results are neither reproducible nor comparable, because the means of infusion, amount of injected cells and clinical variability differ among the studies. To better understand the specific role of HT in the management of end-stage liver disease, it is important that controlled studies, designed on the principles of evidence-based medicine, be done in order to guarantee the reproducibility of results.

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“…Based on this well-known phenomenon, many researchers have proposed and investigated the use of isolated liver parenchymal cells for the treatment of several liver diseases. 5,6 Possible clinical applications are hepatocyte transplantation and treatment with a bioartificial liver loaded with human liver cells. Both methods could not only support, but also be an alternative to liver transplantation, allowing more patients to be treated.…”

mentioning

confidence: 99%

Isolation of human hepatocytes from livers rejected for liver transplantation on a national basis: Results of a 2-year experience

Baccarani

2003

Liver Transplantation

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The offer of liver transplantation to many patients affected by liver failure is limited by organ shortage. Clinical application of human-based liver cell therapies, such as bioartificial liver and hepatocyte transplantation, might support liver transplantation, allowing more patients to be treated and decreasing mortality in the waiting list. The development of a standardized method of hepatocyte isolation is a mainstay for large-scale application of liver cell therapy. The aim of this study is to analyze retrospectively a 2-year experience of human hepatocyte isolation from livers rejected from transplantation at organ harvesting, performed on a national basis in Italy. All the livers judged unsuitable for transplantation were considered for hepatocyte isolation. Macrosteatosis greater than 60% was the most common reason of refusal, followed by nonviral cirrhosis. Fifty-four organs were used. Human hepatocyte isolation resulted in more that 7 million liver cells/g of tissue digested with 73% ؎ 14% viability. Steatotic organs gave better results in terms of cell yield than cirrhotic livers. Isolated hepatocytes were able to perform specific liver functions, and evidence of factor IX and albumin messenger RNA (mRNA) production was reported when cells were plated in culture. Modifications of the traditional method of hepatocyte isolation, aimed at reducing ischemia-reperfusion damage and improving post-isolation cell conditions, showed improvements in post-isolation viability. In conclusion, we show that it is possible to use the vast majority of livers not suitable for transplantation on a national basis for human hepatocyte isolation, obtaining a large amount of viable functioning human hepatocytes that might be used for cell transplantation and therapy. (Liver Transpl 2003;9:506-512.)

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Liver cell transplantation: The road to clinical application

Cited by 67 publications

References 73 publications

Perbandingan Efek Pemberian Emulsi Kedelai dan Emulsi Tempe Terhadap Hitung Limfosit Total pada Tikus Putih yang Telah Diberi Prednison

Perbandingan Efek Pemberian Emulsi Kedelai dan Emulsi Tempe Terhadap Hitung Limfosit Total pada Tikus Putih yang Telah Diberi Prednison

Clinical applications of hepatocyte transplantation

Isolation of human hepatocytes from livers rejected for liver transplantation on a national basis: Results of a 2-year experience

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