Liver-derived cell lines from cavefish Astyanax mexicanus as an in vitro model for studying metabolic adaptation

Abstract

Cell lines have become an integral resource and tool for conducting biological experiments ever since the Hela cell line was first developed (1). They not only allow detailed investigation of molecular pathways but are faster and more cost-effective than most in vivo approaches. The last decade saw many emerging model systems strengthening basic science research. However, lack of genetic and molecular tools in these newer systems pose many obstacles. Astyanax mexicanus is proving to be an interesting new model system for understanding metabolic adaptation. To further enhance the utility of this system, we developed liver-derived cell lines from both surface-dwelling and cave-dwelling morphotypes. In this study, we provide detailed methodology of the derivation process along with a comprehensive biochemical and molecular characterization of the cell lines, which reflects key metabolic traits of cavefish adaptation. We anticipate these cell lines to become a useful resource for the Astyanax community as well as researchers investigating fish biology, comparative physiology, and metabolism.

Metabolic reprogramming underlies cavefish muscular endurance despite loss of muscle mass and contractility

“…The above data agree with van der Weele (2022) and Medley (2020) who found elevated carbohydrate transcripts and metabolites, respectively, within multiple cavefish tissues relative to surface fish. In fact, we recently found primary cavefish liver-derived cells have increased glycolytic capacity relative to surface fish liver-derived cells (Krishnan et al 2022). We propose this is an adaptation to their hypoxic cave environment.…”