Liver-directed gene therapy of diabetic rats using an HVJ-E vector containing EBV plasmids expressing insulin and GLUT 2 transporter

Kg, Park; Morishita, Ryuichi; Kaneda, Yasufumi; Sy, Kim; Song, Dae‐Kyu; Hs, Kim; Cw, Nam; Hc, Lee; Ku, Lee; Jy, Park; Bw, Kim; Kim, J-G.; Ik, Lee

doi:10.1038/sj.gt.3302644

Cited by 12 publications

(4 citation statements)

References 36 publications

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“…This is a surprising result given that there are no explicit diffusivity thresholds within the model setup, and it would thus be interesting to see if such a phase transition actually existed in an experimental setting. One could potentially investigate this by using different chemoattractants with varying intrinsic diffusion coefficients, with the modulation of the uptake of these chemoattractants through genetic engineering of appropriate cell surface receptor systems (for the glucose transport receptor (GLUT), used in here as an example, this has in fact been attempted already (Kim et al, 2006)).…”

Section: Discussionmentioning

confidence: 99%

Cancer cell motility: Optimizing spatial search strategies

et al. 2009

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Aberrantly regulated cell motility is a hallmark of cancer cells. A hybrid agent-based model has been developed to investigate the synergistic and antagonistic cell motility-impacting effects of three microenvironment variables simultaneously: chemoattraction, haptotactic permission, and biomechanical constraint or resistance. Reflecting distinct cell-specific intracellular machinery, the cancer cells are modelled as processing a variety of spatial search strategies that respond to these three influencing factors with differential weights attached to each. While responding exclusively to chemoattraction optimizes cell displacement effectiveness, incorporating permission and resistance components becomes increasingly important with greater distance to the chemoattractant source and/ or after reducing the ligand's effective diffusion coefficient. Extending this to a heterogeneous population of cells shows that displacement effectiveness increases with clonal diversity as characterized by the Shannon index. However, the resulting data can be fit best to an exponential function, suggesting that there is a level of population heterogeneity beyond which its added value to the cancer system becomes minimal as directionality ceases to increase. Possible experimental extensions and potential clinical implications are discussed.

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Section: Discussionmentioning

confidence: 99%

Cancer cell motility: Optimizing spatial search strategies

et al. 2009

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“…In addition, no significant difference was observed between the rat and human CDMs. HVJ-E can be a useful tool for gene transfection into three-dimensional cultured tissues, because HVJ-E has been reported in gene transfection not only into cells in vitro but also into animal tissues in vivo (20,21).…”

Section: Gfp Expression In Fibroblastsmentioning

confidence: 99%

Preparation and Evaluation of Gene-transfected Cultured Skin as a Novel Drug Delivery System for Severely Burned Skin

et al. 2007

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“…At first, HVJ-E was developed as a drug-delivery system for plasmids, siRNAs, proteins, and anticancer drugs due to its membrane fusion ability. 21 – 24 However, it was recently reported that HVJ-E directly induces cancer cell death and the activation of antitumor immunity by itself; since this discovery, research has focused on the mechanism of the antitumor effects induced by HVJ-E treatment.…”

Section: Uv-irradiated Sendai Virus Particlesmentioning

confidence: 99%

Oncolytic Sendai virus-based virotherapy for cancer: recent advances

Saga

Kaneda

2015

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Many drugs have been developed and optimized for the treatment of cancer; however, it is difficult to completely cure cancer with anticancer drugs alone. Therefore, the development of new therapeutic technologies, in addition to new anticancer drugs, is necessary for more effective oncotherapy. Oncolytic viruses are one potential new anticancer strategy. Various oncolytic viruses have been developed for safe and effective oncotherapy. Recently, Sendai virus-based oncotherapy has been reported by several groups, and attention has been drawn to its unique anticancer mechanisms, which are different from those of the conventional oncolytic viruses that kill cancer cells by cancer cell-selective replication. Here, we introduce Sendai virus-based virotherapy and its anticancer mechanisms.

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Liver-directed gene therapy of diabetic rats using an HVJ-E vector containing EBV plasmids expressing insulin and GLUT 2 transporter

Cited by 12 publications

References 36 publications

Cancer cell motility: Optimizing spatial search strategies

Cancer cell motility: Optimizing spatial search strategies

Preparation and Evaluation of Gene-transfected Cultured Skin as a Novel Drug Delivery System for Severely Burned Skin

Oncolytic Sendai virus-based virotherapy for cancer: recent advances

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