Liver histopathology in Baltic eelpout (Zoarces viviparus) – A baseline study for use in marine environmental monitoring

Liver alterations inOreochromis niloticus(Pisces) induced by insecticide imidacloprid: Histopathology and heat shock proteinin situlocalization

“…[53] Increased synthesis and uptake of fatty acids bring with it increased accumulation of lipids in these organs. [54,55] In most species of fish, lipids are predominant sources for obtaining energy, but excessive deposition of lipids can cause problems for the animal's health. [56] Several studies have indicated that exposure of fish to environmental stress can disrupt lipid metabolism, and they observed vacuole accumulation in liver.…”

Development of Tools for Integrated Monitoring and Assessment of Hazardous Substances and Their Biological Effects in the Baltic Sea

“…of Finland, G. of Bothnia, G. of Riga, and G. of Gdansk, Belt SeaBaršienė et al (2012) and Baršienė et al (unpublished)Oil-degrading bacteria in the intestinal tract in bivalves and fishThe presence of oil-degrading bacteria in the intestinal tract was studied in bivalves and fish as a new method for in situ assessment of petroleum hydrocarbon pollution in marine and estuarine environments Mytilus trossulus , Macoma balthica , Zoarces viviparus , Platichthys flesus , Clupea harengus Different subregions of the Baltic SeaBaršienė et al (unpublished; in press)Liver histopathology in fishLiver lesions were studied in eelpout and herring as the first attempt to carry out systematic studies on these species by analyzing samples from all studied subregions by applying diagnostic criteria originally developed for flatfish species (Feist et al 2004; Lang et al 2006) Zoarces viviparus , and Clupea harengus G. of Finland, G. of Bothnia, G. of Riga, and G. of Gdansk Belt SeaFricke et al (2012), Fricke et al (in prep.) and Gschwind (2012)Macrophage aggregates in fishMacrophage aggregates were quantitatively studied in fish liver and spleen Zoarces viviparus , and Platichthys flesus G.…”

“…and Gschwind (2012)Macrophage aggregates in fishMacrophage aggregates were quantitatively studied in fish liver and spleen Zoarces viviparus , and Platichthys flesus G. of Finland, G. of Bothnia, G. of Riga, and G. of Gdansk Belt SeaFricke et al (2012) and Dabrowska et al (2012)Intersex in fishIntersex, regarded as the consequence of an endocrine disruption effects in gonads of male fish, was studied by histological analysis Zoarces viviparus , and Platichthys flesus G. of Finland, G. of Bothnia, G. of Riga, G. of Gdansk, and Belt SeaGercken et al (in prep.…”

“…; Dabrowska et al (2012, 2013)Testing and validation of integrated monitoring approaches, indices, and expert systems with regard to their applicability for the Baltic Sea, taking into account the specific biotic and abiotic characteristics of the different subregions and different contaminant burdensSee above; mussel caging studies in G. of Gdansk and G. of Bothnia; testing of integrated indices (IBR, IBAS) in fish (herring and eelpout); testing of an expert system on amphipods ( Monoporeia affinis ); application of the Fish Disease IndexThis article; subregional assessment articles 2014, in prep. ; Dabrowska et al (2012, 2013), Fricke et al (2012) and Turja et al (in press)Cooperation with the expert groups of ICES and HELCOM for providing recommendations to the ongoing revision of HELCOM monitoring programs, and implementation of the BSAP and MSFDClose collaboration with HELCOM CORESET; exchange of information between relevant ICES expert groups; support to BSAP and MSFD at national levelsICES (2011a, b, 2012) and HELCOM (2012a, b)An integrated multilevel toolbox consisting of established and novel biomarkers as sensitive diagnostic tools to identify how hazardous substances affect the Baltic Sea ecosystem, also in the context of stress due to varying environmental conditions and climate changeSee above (subregional assessments, selection of methods, and determination of assessment criteria); experimental studies on mussels and fishSubregional assessment articles 2014, in prep. ; Dabrowska et al (2012, 2013), Höher et al (2012) and Gorokhova et al (2013)Capacity building and strengthening of networking and quality assurance among Baltic Sea institutions via workshops to exchange, harmonize, and intercalibrate methodologies; development of technical guidelines and Standard operation procedures as well as appropriate trainingIntercalibration and workshops; draft Standard operation procedures, training; networking activitiesKammann et al (2013) and Standard operation procedures (2014, in prep.…”

“…; Dabrowska et al (2012, 2013), Kreitsberg et al (2012), Höher et al (2012), Fricke et al (2012), Baršienė et al (2012), and Berezina et al (2013)Scientifically based recommendations for the set-up of an integrated chemical–biological effects monitoring of hazardous substances in the whole Baltic Sea area, based on subregional assessments for future integrated assessments of Baltic Sea ecosystem healthSee above (subregional assessments; collaboration with ICES SGEH and HELCOM)ICES (2011a) and HELCOM (2012a, b)Generation of baseline data for regions in the Baltic Sea where few or no biological effects data existed and updating of data in other subregionsSee above; BonusHAZ database (jointly with BALCOFISH project) with 60 different parameters for fish (flounder, eelpout, and herring) and invertebrates ( Mytilus sp., Macoma balthica , amphipods, and gastropods)Subregional assessment articles 2014, in prep. ; Barda et al (2013), Fricke et al (2012) Baršienė et al (2012) and Turja et al (in press) Identification of relevant target species for the highly variable Baltic Sea subregionsTesting of local native organisms for assessing the suitability of biological effects methodsSubregional assessment articles 2014, in prep. ; Barda et al (2013)Determination of subregional reference/target/effect levels and collection of data for whole-region assessment of biological effectsSelection of CORESET biological effects indicators for hazardous substances (lysosomal membrane stability in fish, bivalves, or amphipods; induction of micronuclei in fish, bivalves, or amphipods; embryo aberrations in fish [eelpout] or amphipods; Fish Disease Index; imposex in marine gastropods (TBT indicator); PAH metabolites in fish [PAH indicator]); Numeric Assessment Criteria for Baltic Sea organisms to assess biological effects; overview of Core and selected Candidate Indicators to assess the effects of hazardous substances at different biological levels; ICES SGEH Biological Effects methods’ Background Documents for the Baltic Sea region (9 methods)ICES (2011a), HELCOM (2012a, b) and Baršienė et al (2012)Linking early effects and higher level effects by relating responses directly to changes in growth, reproductive output, or energy utilizationExperimental and field studies with mussels ( Mytilus trossulus ) and amphipods ( Monoporeia affinis )Turja et al (in press) and Löf et al (in prep.…”