Liver lipidomics analysis reveals the anti-obesity and lipid-lowering effects of gypnosides from heat-processed Gynostemma pentaphyllum in high-fat diet fed mice

Xie, Peng; Xie, Jinbo; Xiao, Man-Yu; Guo, Mei; Qi, Yan-Shuang; Li, Fangfang; Piao, Xiang‐Lan

doi:10.1016/j.phymed.2023.154834

Cited by 9 publications

(4 citation statements)

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“…B. et al, 2023 ). Furthermore, it has been found to restore sphingolipid and glycerophospholipid metabolism in mice with glucose metabolism disorders ( Xie P. et al, 2023 ). In conjunction with this study, which showed that GPs elevate the expression of hepatic Ces1d in liver-injured rats, indicating that GPs may restore glucose and lipid metabolism by modulating CES1.…”

Section: Discussionmentioning

confidence: 99%

Label-free proteomic analysis reveals the hepatoprotective mechanism of gypenosides in liver injury rats

Chen,

Ma,

Wang

et al. 2024

Front. Pharmacol.

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Chronic liver disease, a long-term condition resulting from various causes such as alcohol abuse, metabolic disorders, and viral hepatitis, is becoming a significant global health challenge. Gypenosides (GPs), derived from the traditional Chinese medicine Gynostemma pentaphyllum (Thunb.) Makino, exhibited hepatoprotective properties in recent years, yet the precise therapeutic mechanism remains unclear. In this study, label-free and parallel reaction monitoring (PRM) proteomics were used to elucidate the hepatoprotective mechanism of GPs in liver injury rats. Through label-free proteomics, we identified 2104 differentially expressed proteins (DEPs) associated with liver injury, along with 1974 DEPs related to the effects of GPs. Bioinformatics analysis revealed that GPs primarily restored metabolic processes involving valine, leucine, and isoleucine degradation, as well as propanoate and butanoate metabolism, and steroid hormone biosynthesis during liver injury. Subsequently, overlapping the two groups of DEPs identified 1508 proteins reversed following GPs treatment, with key targets further validated by PRM. Eight target proteins were identified for GPs treatment of liver injury, including Lgals3, Psat1, Phgdh, Cyp3a9, Cyp2c11, Cyp4a2, Glul, and Ces1d. These findings not only elucidated the hepatoprotective mechanism of GPs, but may also serve as potential therapeutic targets of chronic liver disease.

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Section: Discussionmentioning

confidence: 99%

Label-free proteomic analysis reveals the hepatoprotective mechanism of gypenosides in liver injury rats

Chen,

Ma,

Wang

et al. 2024

Front. Pharmacol.

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“…HGyp samples were prepared by our laboratory ( Xie et al, 2022 ; Xie et al, 2023 ). The leaves of G. pentaphyllum were steamed at 120°C under 0.24 MPa for 3 h. After heat processing, they were reflux extracted with 80% ethanol (1:8, w/v) for 2 h for three times and then concentrated by rotary evaporator under reduced pressure.…”

Section: Methodsmentioning

confidence: 99%

“…Gypenosides after heat-processing (HGyp) have been suggested to exert various stronger biological effects, such as, antioxidant, anti-obesity and anti-tumor activities ( Xing et al, 2019 ; Liu et al, 2021 ; Zu et al, 2021 ). At present, the effects of G. pentaphyllum on protecting overweight, hyperglycemia and hyperlipidemia have been reported ( Norberg et al, 2004 ; Liu et al, 2017 ; Xie et al, 2023 ), while the research on the AGE-RAGE signaling pathway is only an in vitro experiment ( Zhang H. et al, 2018 ), and there are no in vivo studies reported. Therefore, this study investigated the potential protective and antioxidant effects of HGyp on high fat diet-induced GMD in a mouse model combined with the blood components of HGyp and network pharmacology.…”

Section: Introductionmentioning

confidence: 99%

Protective effect of heat-processed Gynostemma pentaphyllum on high fat diet-induced glucose metabolic disorders mice

Xie,

Guo

et al. 2023

Front. Pharmacol.

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Glucose metabolic disorders (GMD) can promote insulin resistance (IR) and diabetes, and damage liver and kidney. Gynostemma pentaphyllum is commonly used in the clinical treatment of diabetes, but the research on its main active constituents and GMD has not been reported yet. This study explores the therapeutic potential of gypenosides of heat-processed Gynostemma pentaphyllum (HGyp) on high-fat diet-induced GMD in mice. HGyp was administered at different doses for 12 weeks. The investigation encompassed an array of parameters, including body weight, blood lipids, blood glucose, and liver tissue components. Metabolomic and network analyses were conducted to uncover potential targets and pathways associated with HGyp treatment. The results revealed that HGyp alleviated GMD by reducing body weight, blood glucose, and improving blood lipids levels, while increasing liver glycogen and antioxidant enzyme levels. Additionally, HGyp exhibited protective effects on liver and kidney health by reducing tissue damage. Fourteen blood components were detected by LC-MS. Metabolomic and network analyses indicated the potential engagement of the AGE-RAGE signaling pathway in the therapeutic effects of HGyp.Furthermore, Western blot and ELISA assays confirmed that HGyp upregulated GLO1 and GLUT4 while down-regulating AGEs and RAGE expression in liver tissue. In light of these findings, HGyp demonstrates promise as a potential therapeutic candidate for combating GMD, warranting further exploration in the development of therapeutic strategies or functional products.

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“…Gynostemma pentaphyllum (Thunb.) Makino, an herbaceous perennial plant of the Cucurbitaceae family, has a rich history in traditional Chinese medicine and is widely consumed as an herbal tea in Asian countries for its potential to prevent obesity [ 22 ]. The leaves of G. pentaphyllum plants, particularly the tender leaves, are the primary site for the synthesis of gypenosides, a class of dammarane-type triterpenoid saponins [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

Genome-wide characterization of the bHLH gene family in Gynostemma pentaphyllum reveals its potential role in the regulation of gypenoside biosynthesis

Qin,

Li,

Chen

et al. 2024

BMC Plant Biol

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Background Gynostemma pentaphyllum, an ancient Chinese herbal medicine, serves as a natural source of gypenosides with significant medicinal properties. Basic helix-loop-helix (bHLH) transcription factors play pivotal roles in numerous biological processes, especially in the regulation of secondary metabolism in plants. However, the characteristics and functions of the bHLH genes in G. pentaphyllum remain unexplored, and their regulatory role in gypenoside biosynthesis remains poorly elucidated. Results This study identified a total of 111 bHLH members in G. pentaphyllum (GpbHLHs), categorizing them into 26 subgroups based on shared conserved motif compositions and gene structures. Collinearity analysis illustrated that segmental duplications predominately lead to the evolution of GpbHLHs, with most duplicated GpbHLH gene pairs undergoing purifying selection. Among the nine gypenoside-related GpbHLH genes, two GpbHLHs (GpbHLH15 and GpbHLH58) were selected for further investigation based on co-expression analysis and functional prediction. The expression of these two selected GpbHLHs was dramatically induced by methyl jasmonate, and their nuclear localization was confirmed. Furthermore, yeast one-hybrid and dual-luciferase assays demonstrated that GpbHLH15 and GpbHLH58 could bind to the promoters of the gypenoside biosynthesis pathway genes, such as GpFPS1, GpSS1, and GpOSC1, and activate their promoter activity to varying degrees. Conclusions In conclusion, our findings provide a detailed analysis of the bHLH family and valuable insights into the potential use of GpbHLHs to enhance the accumulation of gypenosides in G. pentaphyllum.

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Liver lipidomics analysis reveals the anti-obesity and lipid-lowering effects of gypnosides from heat-processed Gynostemma pentaphyllum in high-fat diet fed mice

Cited by 9 publications

References 50 publications

Label-free proteomic analysis reveals the hepatoprotective mechanism of gypenosides in liver injury rats

Label-free proteomic analysis reveals the hepatoprotective mechanism of gypenosides in liver injury rats

Protective effect of heat-processed Gynostemma pentaphyllum on high fat diet-induced glucose metabolic disorders mice

Genome-wide characterization of the bHLH gene family in Gynostemma pentaphyllum reveals its potential role in the regulation of gypenoside biosynthesis

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