Liver-lobule-mimicking patterning via dielectrophoresis and hydrogel photopolymerization

Chen, Yu-Shih; Tung, Chen-Kuo; Dai, Tzu-Hsuan; Wang, Xiaohong; Yeh, Chih-Ting; Fan, Shih-Kang; Liu, Cheng-Hsien

doi:10.1016/j.snb.2021.130159

Cited by 16 publications

(4 citation statements)

References 58 publications

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“…Other approaches use non-uniform electric fields to selectively guide cell positioning into complex tissue patterns (such as liver lobules) onto an electrode-containing substrate. 22 However, substrate requirements for these techniques limit their integration with compliant hydrogels or other favorable cell culture substrates.…”

Section: Introductionmentioning

confidence: 99%

Independent control over cell patterning and adhesion on hydrogel substrates for tissue interface mechanobiology

Prahl¹,

Porter²,

Liu³

et al. 2023

iScience

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Section: Introductionmentioning

confidence: 99%

Independent control over cell patterning and adhesion on hydrogel substrates for tissue interface mechanobiology

Prahl¹,

Porter²,

Liu³

et al. 2023

iScience

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“…Since the bioassembly technique can directly manipulate millions of live cells to form multicellular structures with close intercellular proximity, it improves contact-dependent cell communication and promotes the emergence of tissue-specific functions [16][17][18] . Currently, a variety of bioassembly techniques have been demonstrated in tissue construction by exploring the interactions between force fields and live cells such as electric field [19] , magnetic field [20,21] , and acoustic field [22][23][24] . Particularly, several acoustic bioassembly techniques based on Faraday wave [25,26] , bulk acoustic wave [27][28][29] , and surface acoustic wave [30,31] have been increasingly reported in biofabrication due to their advantages of high tunability, biocompatibility, and efficiency [24] .…”

Section: Introductionmentioning

confidence: 99%

Multifrequency control of Faraday wave bioassembly for constructing multiscale hPSC-derived neuronal networks

Gu,

Zhao,

Fan

et al. 2023

Preprint

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Bioassembly is recently regarded as a critical alternative biofabrication technical route to bioprinting since it can directly manipulate millions of live cells to form multicellular structures with close intercellular proximity, improving contact-dependent cell communication and promoting the emergence of tissue-specific functions. However, acoustic bioassembly techniques are currently limited to generating cytoarchitecture with a single characteristic length which cannot faithfully mimic the multiscale cellular structures in native tissues. To overcome this challenge, herein we report a novel acoustic bioassembly technique that employs multifrequency control of Faraday waves to form multiscale cellular structures. By superimposing multiple sine wave signals with proper amplitude ratios, Faraday waves containing multiple wavelengths can be induced and enabled to generate multiscale structures in few seconds. Using this technique, we construct functional neuronal networks with multiscale connectivity that display spontaneous neuroelectrical activities. We anticipate this technique will find wide applications in tissue engineering and regenerative medicine.

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“…The manipulation of cells and cellular objects is one of the most promising methods applied in biomedical researches such as drug delivery [ 1 ], tissue engineering [ 2 ], personalized diagnosis, and treatment [ 3 ]. As a typical cell manipulation methodology, cell transport represents a series of significant tasks in biomedical researches, such as cell isolation [ 4 ], cell patterning [ 5 ], and cell assembly [ 6 ]. For example, to construct a drug test model or clinical alternatives with physiological properties more similar to liver hepatic lobules, it is incredibly beneficial to transport parenchymal cells and nonparenchymal cells and arrange them alternately regionalized with an outline of a six-sided cylinder and radial distribution.…”

Section: Introductionmentioning

confidence: 99%

POMDP-Based Real-Time Path Planning for Manipulation of Multiple Microparticles via Optoelectronic Tweezers

et al. 2022

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With high throughput and high flexibility, optoelectronic tweezers (OETs) hold huge potential for massively parallel micromanipulation. However, the trajectory of the virtual electrode has been planned in advance in most synchronous manipulations for multiple targets based on an optically induced dielectrophoresis (ODEP) mechanism, which is insufficient to ensure the stability and efficiency in an environment with potential collision risk. In this paper, a synchronously discretized manipulation method based on a centralized and decoupled path planner is proposed for transporting microparticles of different types with an OET platform. An approach based on the Kuhn-Munkres algorithm is utilized to achieve the goal assignment between target microparticles and goal positions. With the assistance of a visual feedback module, a path planning approach based on the POMDP algorithm dynamically determines the motion strategies of the particle movement to avoid potential collisions. The geometrical parameters of the virtual electrodes are optimized for different types of particles with the goal of maximum transport speed. The experiments of micropatterning with different morphologies and transporting multiple microparticles (e.g., polystyrene microspheres and 3T3 cells) to goal positions are performed. These results demonstrate that the proposed manipulation method based on optoelectronic tweezers is effective for multicell transport and promises to be used in biomedical manipulation tasks with high flexibility and efficiency.

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Liver-lobule-mimicking patterning via dielectrophoresis and hydrogel photopolymerization

Cited by 16 publications

References 58 publications

Independent control over cell patterning and adhesion on hydrogel substrates for tissue interface mechanobiology

Independent control over cell patterning and adhesion on hydrogel substrates for tissue interface mechanobiology

Multifrequency control of Faraday wave bioassembly for constructing multiscale hPSC-derived neuronal networks

POMDP-Based Real-Time Path Planning for Manipulation of Multiple Microparticles via Optoelectronic Tweezers

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