Liver mRNA expression of IGF-I and IGFBPs in adult undernourished diabetic rats

Goya, Luis; Rivero, Francisco; Martín, M. Antonia; Álvarez, Carmen; Ramos, Sonia; Puente, Ana de la; Pascual-Leone, A. M.

doi:10.1016/s0024-3205(99)00177-0

Cited by 7 publications

(8 citation statements)

References 34 publications

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“…Our study, using RT-PCR technique, demonstrated the early reduction in IGF-1 mRNA contents in liver tissues of alloxan-induced diabetic rats, which was consistent with the previous studies using Northern blot and RT-PCR [5,6] . However, Veronica MC and Goya et al did not study the changes in liver tissue IGF-1 protein and effect of different blood glucose level and duration.…”

Section: Discussionsupporting

confidence: 92%

“…Some studies showed that tissue IGF-1 gene expression might be affected by systemic or local factors or both in diabetes, i.e. decrease of GH receptors in target cells and its binding affinity [13] , and by reduced or absent pulsatile pattern secretion of GH, metabolic abnormality of insulin like growth factor binding proteins (IGFBPs) [6,14] , negative nitrogen balance [15,16] etc. All these may probably lead to a decline of IGF-1.…”

Section: Discussionmentioning

confidence: 99%

“…In diabetes, IGF-1 in most tissues were down regulated at different degrees, varying with specific tissues [1,5,18,19] . In the liver it was down regulated [1,6] . Insulin that corrects hyperglycemia can correct the abnormal IGF-1 gene expression.…”

Section: Discussionmentioning

confidence: 99%

“…Liver might be the main source of circulating IGF-1 [1,5] . Recent studies have shown that there was early reduction in hepatic tissue IGF-1 gene expression in experimental diabetes [6] . However, further investigation on it is lacking.…”

Section: Introductionmentioning

confidence: 99%

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Expression of liver insulin-like growth factor 1 gene and its serum level in rats with diabetes

Wang²,

Chen³

et al. 2004

WJG

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Expression of liver insulin-like growth factor 1 gene and its serum level in rats with diabetes

Wang²,

Chen³

et al. 2004

WJG

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“…In agreement, we now show that undernutrition of peripubertal rats significantly reduced body weight, which at this age is directly proportional to linear growth (33). Circulating IGF-I and liver IGF-I mRNA levels were significantly reduced, as reported in adults (5,6,8,9,25), and this could explain the decreased longitudinal growth.…”

Section: Discussionsupporting

confidence: 89%

Interaction between malnutrition and ovarian hormones on the systemic IGF-I axis

Goya

García‐Segura²,

Ramos³

et al. 2002

European Journal of Endocrinology

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Objective: In malnutrition both the GH -IGF and reproductive axes are greatly affected. Because the actions of IGF and sex steroids are inter-dependent in many tissues, we have examined how ovariectomy modulates the response of the systemic IGF system to undernutrition. Design and methods: Peripubertal (30 days of age) female rats were either sham operated or ovariectomized. Five days later half of each group was submitted to a protein-caloric restriction diet. All rats were killed at 60 days of age. Results: Growth was decreased in all rats submitted to calorie restriction and this was consistent with a decrease in circulating IGF-I concentrations and liver IGF-I mRNA expression. While in normally fed rats ovariectomy had no significant effect on serum IGF-I concentrations, ovariectomized and underfed rats had significantly higher levels than intact underfed rats. In undernourished rats, serum IGF-binding proteins (IGFBP)-1, -2 and -3 concentrations were significantly reduced and this was not modified by ovariectomy. In contrast, liver mRNA concentrations of IGFBP-1 and -2 were increased and IGFBP-3 unchanged in intact undernourished animals, suggesting that undernutrition could be affecting the proteolysis of these binding proteins, and this response was significantly modulated by ovariectomy. Conclusion: These results indicate that the presence of circulating ovarian hormones significantly affects the response of the IGF system to undernutrition.

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A biodegradable copolymer for the slow release of growth hormone expedites scarring in diabetic rats

et al. 2006

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In many diseases wound healing is impaired. This study was designed to establish whether the healing process in diabetes could be improved using a site-specific polymer delivery system containing hGH. The system was first optimized in in vitro experiments performed on cultured fibroblasts taken from healthy and diabetic rats and then tested in an incisional wound model created in the diabetic Wistar rat. In the in vitro experiments using cultured fibroblasts, cell viability, growth, and proliferation were determined, along with polymer degradation, hormone release rates and the expression of TGFbeta1 in the culture medium. For the in vivo experiments, polymer discs with/without GH were inserted through 3 cm incisions made on the backs of the animals. Wound specimens were obtained 7 and 30 days after surgery to evaluate inflammatory/apoptotic cells, metalloprotease expression and neoangiogenesis using microscopy and immunohistochemical techniques. The local administration of GH using a polymer delivery system did not affect the normal wound healing process. Conversely, when used in diabetic animals, epidermal and dermal repair was expedited. Our findings indicate that GH induces cell proliferation, enhances CD4(+) infiltration; increases extracellular matrix protein deposition; stimulates angiogenesis; and diminishes apoptosis at the diabetic wound site. These effects give rise to a comparable wound healing process to that observed in healthy animals.

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Liver mRNA expression of IGF-I and IGFBPs in adult undernourished diabetic rats

Cited by 7 publications

References 34 publications

Expression of liver insulin-like growth factor 1 gene and its serum level in rats with diabetes

Expression of liver insulin-like growth factor 1 gene and its serum level in rats with diabetes

Interaction between malnutrition and ovarian hormones on the systemic IGF-I axis

A biodegradable copolymer for the slow release of growth hormone expedites scarring in diabetic rats

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