Liver-on-a-Chip‒Magnetic Nanoparticle Bound Synthetic Metalloporphyrin-Catalyzed Biomimetic Oxidation of a Drug in a Magnechip Reactor

Abstract

Biomimetic oxidation of drugs catalyzed by metalloporphyrins can be a novel and promising way for the effective and sustainable synthesis of drug metabolites. The immobilization of 5,10,15,20-tetrakis(2,3,4,5,6-pentafluorophenyl)iron(II) porphyrin (FeTPFP) and 5,10,15,20-tetrakis-(4-sulfonatophenyl)iron(II) porphyrin (FeTSPP) via stable covalent or rapid ionic binding on aminopropyl-functionalized magnetic nanoparticles (MNPs-NH2) were developed. These immobilized catalysts could be efficiently applied for the synthesis of new pharmaceutically active derivatives and liver related phase I oxidative major metabolite of an antiarrhythmic drug, amiodarone integrated in a continuous-flow magnetic chip reactor (Magnechip).

Immobilization of the Aspartate Ammonia‐Lyase from Pseudomonas fluorescens R124 on Magnetic Nanoparticles: Characterization and Kinetics

“…Inspired by our previous studies on immobilization of different enzymes on MNPs as useful carriers for the enzymes and cofactors[ 29 , 39 , 40 , 41 , 42 ] covalent immobilization of PfAAL has been performed on MNPs etched by aminopropyl(trimethoxy)silane and activated by glycerol diglycidyl ether and glycidol in 1 : 1 molar ratio (Figure 1 ). Glycidol was used as a thinning component in surface functionalization.…”

The Fabrication and Application Mechanism of Microfluidic Systems for High Throughput Biomedical Screening: A Review

“…Microfluidic chips are extensively applied in in-vitro biomedical models because of their flexibility, such as having control over fluid and gas flow, and a chip architecture able to replace the classical 2D and 3D cell cultures [1]. Many organ models were established in recent years, based on the microfluidic platform, such as brain-on-a-chip, liver-on-a-chip, lung-on-a-chip, and breast-on-a-chip [2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19]. Body-on-a-chip is designed to include more than one organ on one chip [20].…”

Magnetic Nanoparticles with Dual Surface Functions—Efficient Carriers for Metalloporphyrin-Catalyzed Drug Metabolite Synthesis in Batch and Continuous-Flow Reactors

“…In our previous study, we anchored meso-tetra(pentafluorophenyl)iron porphyrin by covalent bond and meso-tetra(4-sulphonatophenyl)iron porphyrin by ionic bond onto simple aminopropyl-grafted MNPs. These immobilized catalyst systems were used under continuous-flow reaction conditions to synthesize Amiodarone metabolites [25].…”