Liver Parenchymal Cell Injury

Reynolds, Edward S.

doi:10.1083/jcb.19.1.139

Cited by 241 publications

(60 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Omission of calcium from the standard fixative or use of phosphate-buffered osmium tetroxide (19) failed to preserve the electron-opaque deposits formed within the mitochondrial matrix following poisoning with carbon tetrachloride. In addition, cubes of liver quenched in liquid propane at 90°K were either freeze-dried (220°K) (20) or freeze-substituted (190°K) in acetone, or in acetone containing 1 per cent osmium tetroxide. Upon completion, tissues were warmed to room temperature and embedded in Epon.…”

Section: Methodsmentioning

confidence: 99%

“…Cryostat and plastic sections of liver were stained with alizarin red S for calcium (20,24) and with lead nitrate for inorganic phosphate (25).…”

Section: Methodsmentioning

confidence: 99%

“…Damaged function of this vital structure, either as a result of direct attack of the hepatotoxin (20) or indirectly through destruction of the protein synthetic properties of the granular endoplasmic reticulum (41) or ATP depletion would tend to result in an increased permeability of the membrane to calcium.…”

Section: Figure 19mentioning

confidence: 99%

See 2 more Smart Citations

Liver Parenchymal Cell Injury

Reynolds

1965

The Journal of Cell Biology

Self Cite

View full text Add to dashboard Cite

Accumulation of calcium in the mitochondria of rat liver parenchymal cells at 16 and 24 hours after poisoning with carbon tetrachloride is associated with an increase in amount of liver inorganic phosphate, the persistence of mitochondrial adenosine triphosphatase activity, and the formation of electron-opaque intramitochondrial masses in cells with increased calcium contents. These masses, which form within the mitochondrial matrix adjacent to internal mitochondrial membranes, resemble those observed in isolated mitochondria which accumulate calcium and inorganic phosphate; are present in a locus similar to that of electron opacities which result from electron-histochemical determination of mitochondrial ATPase activity; and differ in both appearance and position from matrix granules of normal mitochondria. After poisoning, normal matrix granules disappear from mitochondria prior to their accumulation of calcium. As calcium-associated electron-opaque intramitochondrial masses increase in size, mitochondria degenerate in appearance. At the same time, cytoplasmic membrane systems of mid-zonal and centrilobular cells are disrupted by degranulation of the rough endoplasmic reticulum and the formation of labyrinthine tubular aggregates. The increase in amount of inorganic phosphate in rat liver following poisoning is balanced by a decreased amount of phosphoprorein. These chemical events do not appear to be related, however, as the inorganic phosphate accumulated is derived from serum inorganic phosphate.

show abstract

Section: Methodsmentioning

confidence: 99%

“…Cryostat and plastic sections of liver were stained with alizarin red S for calcium (20,24) and with lead nitrate for inorganic phosphate (25).…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Liver Parenchymal Cell Injury

Reynolds

1965

The Journal of Cell Biology

Self Cite

View full text Add to dashboard Cite

show abstract

“…Sections were stained for 30 min at 60°C with 1% sodium borate saturated with uranyl acetate, and then with lead citrate according to the procedure of Reynolds (19).…”

Section: Electron Microscopymentioning

confidence: 99%

Enzymatic Properties of the Inner and Outer Membranes of Rat Liver Mitochondria

Schnaitman¹,

Greenawalt²

1968

The Journal of Cell Biology

1,382

478

View full text Add to dashboard Cite

Treatment of rat liver mitochondria with digitonin followed by differential centrifugation was used to resolve the intramitochondrial localization of both soluble and particulate enzymes. Rat liver mitochondria were separated into three fractions: inner membrane plus matrix, outer membrane, and a soluble fraction containing enzymes localized between the membranes plus some solublized outer membrane. Monoamine oxidase, kynurenine hydroxylase, and rotenone-insensitive NADH-cytochrome c reductase were found primarily in the outer membrane fraction. Succinate-cytochrome c reductase, succinate dehydrogenase, cytochrome oxidase, j-hydroxybutyrate dehydrogenase, a-ketoglutarate dehydrogenase, lipoamide dehydrogenase, NAD-and NADH-isocitrate dehydrogenase, glutamate dehydrogenase, aspartate aminotransferase, and ornithine transcarbamoylase were found in the inner membrane-matrix fraction. Nucleoside diphosphokinase was found in both the outer membrane and soluble fractions; this suggests a dual localization. Adenylate kinase was found entirely in the soluble fraction and was released at a lower digitonin concentration than was the outer membrane; this suggests that this enzyme is localized between the two membranes. The inner membrane-matrix fraction was separated into inner membrane and matrix by treatment with the nonionic detergent Lubrol, and this separation was used as a basis for calculating the relative protein content of the mitochondrial components. The inner membrane-matrix fraction retained a high degree of morphological and biochemical integrity and exhibited a high respiratory rate and respiratory control when assayed in a sucrose-mannitol medium containing EDTA.

show abstract

“…In a later stage or with a larger dose of CCl4, however, a considerable decrease of the activity is reported (25,28,29). According ly, the decrease of microsomal glucose-6-phosphatase activity in viva may be produced by CCl4 per se as well as its more toxic metabolite as suggested by other workers (20,21,30).…”

Section: Discussionmentioning

confidence: 99%