Liver plasma membranes from essential fatty acid-deficient rats

Brivio-Haugland, R.P.; Louis, S. L.; Musch, K.; Waldeck, Nathan; Williams, M. A.

doi:10.1016/0005-2736(76)90184-x

Cited by 97 publications

(5 citation statements)

References 53 publications

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“…In contrast, exposure of cells to oleic acid had little or no effect on adenylate cyclase activity and exposure of cells to stearic acid decreased adenylate cyclase activity; thus, fatty acids exhibit specificity in altering the activity of adenylate cyclase. These observations agree well with the previous reports by Brivio-Haugland et al (1976), which show that adenylate cyclase activity is decreased in cell mem- Lipid-free serum + linoleic acid norepinephrine 19.9 5 0.9 229 (I Cells were grown for a total of 3 days in medium A containing 5% fetal bovine serum or lipid-free fetal bovine serum; 16 pM-linoleic acid was added twice a day during the first 2 days and 3 times a day during the 3rd day. The medium was changed every 24 h. Cells (10 mg protein per 75-cm2 flask) were harvested after the 3rd day.…”

Section: Discussionsupporting

confidence: 94%

Lipid Requirement for Long‐lived Morphine‐dependent Activations of Adenylate Cyclase of Neuroblastoma × Glioma Hybrid Cells

Wilkening

Nirenberg

1980

Journal of Neurochemistry

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Morphine or norepinephrine at 10 p~ inhibit adenylate cyclase of NG108-15 neuroblastoma x glioma hybrid cells and gradually, over a period of 12 h or more, elicit a compensatory, persistent increase in adenylate cyclase activity. The cells are then dependent upon the receptor ligand for maintenance of normal cyclic AMP levels. Serum lipids or linoleic acid are required for the full expression of the long-lived morphine-dependent increase in adenylate cyclase activity, but serum lipids are not required for inhibition of the enzyme by morphine. Serum lipids are also not required for the full expression of the norepinephrine-dependent increase in adenylate cyclase activity. In the NG108-15 model system, therefore, the acquisition of dependence upon morphine can be dissociated from the inhibition of adenylate cyclase by morphine as well as from the acquisition of dependence upon another receptor ligand, norepinephrine.

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Section: Discussionsupporting

confidence: 94%

Lipid Requirement for Long‐lived Morphine‐dependent Activations of Adenylate Cyclase of Neuroblastoma × Glioma Hybrid Cells

Wilkening

Nirenberg

1980

Journal of Neurochemistry

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“…The role of lipid microviscosity in regulation of the plasma membrane physiological as well as pathological behavior has obtained increasing attention in recent years (Shinitzky, 1976;Cooper, 1977). In this connection the C/PL ratio together with the degree of unsaturation of phospholipids in plasma membranes appears to play a major role in modulating membrane fluidity, as well as the activity of membrane-bound enzymes (Emmelot & van Hoeven, 1975;Brivio-Haugland et al, 1976). Therefore, the possibility of modulating plasma membrane microviscosity by altering the C/PL ratio in vitro with a simple technique (Shinitzky, 1978) offered us a useful tool to demonstrate the consistency of the reported effect of insulin on liver plasma membrane; the hormone is not effective in increasing plasma membrane microviscosity when this parameter has been already increased well above its physiological level.…”

Section: Discussionmentioning

confidence: 99%

Gross structural changes in isolated liver cell plasma membranes upon binding of insulin

Luly¹,

Shinitzky²

1979

Biochemistry

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The addition of 10(-9) M insulin to a suspension of rat liver plasma membranes increases the overall lipid microviscosity, eta, by about 10--20%. The effect is confined to physiological concentrations of the hormone and is highly specific. The specificity was demonstrated in experiments where insulin analogues were added to liver plasma membranes and where insulin was added to human erythrocyte membranes. In both of these experiments practically no change in eta was detected. Upon in vitro enrichment of the membrane cholesterol, eta exceeded the level mediated by insulin binding, and the addition of 10(-9) M insulin to the cholesterol-enriched membranes did not further increase eta. Concomitant to the increase in eta upon insulin binding, the overall degree of exposure of the membrane protein, presumably to both sides of the membrane, is substantially increased. This effect is in line with the notion of vertical displacement of membrane proteins induced by changes in eta. The observed structural modulation can account for the effect of insulin on unrelated membrane responses, as well as for the negative cooperativity of insulin binding.

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“…Further, the effects of changes in the content of unsaturated fatty acids on membrane enzymes such as ATPase and adenylate cyclase have been studied [30]. Essential fatty acid-deficient rat liver plasma membrane (Na + + K + )-ATPase showed a higher V max and K m compared with controls [31]. In erythrocyte membranes, the Ca 2+ -ATPase V max was higher for corn oil-fed rats compared with lard-fed rats [32].…”

Section: Discussionmentioning

confidence: 99%

Antihypertensive Effect of All-cis-5,8,11,14,17-Icosapentaenoate of Aged Rats Is Associated with an Increase in the Release of ATP from the Caudal Artery

Hashimoto¹,

Shinozuka²,

Hossain³

et al. 1998

J Vasc Res

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Fish oils have been shown to lower blood pressure in hypertensive subjects. All-cis-5,8,11,14,17-icosapentaenoate (EPA), one of the ω–3 polyunsaturated fatty acids, is known to be one of the major active components in fish oil that has beneficial effects on the cardiovascular system. However, little is known about the antihypertensive effect of EPA alone on blood pressure. In the present study, we have determined the spontaneous and noradrenaline-evoked release of ATP, ADP, AMP, and adenosine from caudal arteries of aged (100 weeks old) Wistar rats which were fed a standard diet or a high cholesterol diet, treated with EPA. Dietary EPA administration increased plasma and caudal arterial EPA concentrations and repressed increases in blood pressure with advancing age in both aged rats with and without hypercholesterolemia. In addition, noradrenaline (1 µmol/l) evoked a significantly greater release of purines from the caudal arteries of EPA-administered aged rats compared to both sets of control rats. Regression analysis revealed a significant relationship between the total amount of purines released from the artery and blood pressure. These results suggest that administration of EPA to aged rats increases the release of ATP from the vascular endothelial cells, leading to repression of the blood pressure rise seen with advancing age.

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Liver plasma membranes from essential fatty acid-deficient rats

Cited by 97 publications

References 53 publications

Lipid Requirement for Long‐lived Morphine‐dependent Activations of Adenylate Cyclase of Neuroblastoma × Glioma Hybrid Cells

Lipid Requirement for Long‐lived Morphine‐dependent Activations of Adenylate Cyclase of Neuroblastoma × Glioma Hybrid Cells

Gross structural changes in isolated liver cell plasma membranes upon binding of insulin

Antihypertensive Effect of All-cis-5,8,11,14,17-Icosapentaenoate of Aged Rats Is Associated with an Increase in the Release of ATP from the Caudal Artery

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