Liver ploidy

Feldmann, Gérard

doi:10.1016/s0168-8278(05)80087-x

“…cell population, which reflects contamination by nonneoplastic elements (mononuclear cells, fibroblasts, en-In the normal human adult liver, hepatocytes are diploid or tetraploid, 23,24,36 and our studies by FISH dothelial cells, and perhaps also normal peritumoral hepatocytes). confirmed that a mixture of diploid and tetraploid hepatocytes exist in the normal liver such as we observed A loss of chromosome 17 was observed in our case of focal nodular hyperplasia (tumor 1).…”

Section: Discussionmentioning

confidence: 70%

Interphase cytogenetic studies of human hepatocellular carcinomas by fluorescent in situ hybridization

HAMONBENAIS¹

1996

View full text Add to dashboard Cite

Although numerous allelic chromosome losses have been reported in hepatocellular carcinomas (HCC), chromosome analysis by cytogenetic methods has rarely been performed in these tumors, unlike other solid malignant tumors. The purpose of the current study was to analyze primary liver tumors by conventional cytogenetic methods and by a new molecular cytogenetic technique, called fluorescent in situ hybridization (FISH), a technique that has been recently proposed to count the number of chromosome copies in interphase nuclei with chromosome centromeric probes. Primary cultures of tumoral cells were prepared to obtain metaphases. Specific chromosomes probes 7, 17, and 20 were used to perform in situ hybridization on isolated intact tumoral cells. Seven cases of primary liver tumors (six cases of HCC and one case of benign focal hepatic nodular hyperplasia) were investigated. A few metaphases were obtained in five of the seven tumors, and in most cases numerical abnormalities were difficult to interpret. In contrast with in situ hybridization, all cases of HCC showed losses and/or gains of chromosomes. Loss of one to three chromosomes occurred in five tumors. A gain of two chromosomes was observed in two of these five tumors. In only one case, a gain of only three chromosomes occurred. In addition, a loss of chromosome 17 was recorded for the benign tumor. These results demonstrate that FISH with specific probes can provide information on chromosome number in the tumoral cells of primary liver tumors even in the absence of analyzable metaphases. This technique opens new possibilities for the investigation of chromosome abnormalities in HCC.

show abstract

“…This variation reflects polyploidy, the DNA content increasing correspondingly. 107 At birth, in man, nearly all hepatocytes are diploid (and mononucleate). From the 8th year, when more than 90% of hepatocytes are diploid, the number of tetraploid nuclei (i.e.…”

Section: Nucleusmentioning

confidence: 99%

Anatomy, pathophysiology and basic mechanisms of disease

Crawford¹,

Burt²

2012

MacSween's Pathology of the Liver

View full text Add to dashboard Cite

“…cell population, which reflects contamination by nonneoplastic elements (mononuclear cells, fibroblasts, enIn the normal human adult liver, hepatocytes are diploid or tetraploid, 23,24,36 and our studies by FISH dothelial cells, and perhaps also normal peritumoral hepatocytes). confirmed that a mixture of diploid and tetraploid hepatocytes exist in the normal liver such as we observed A loss of chromosome 17 was observed in our case of focal nodular hyperplasia (tumor 1).…”

Section: Technical Problemsmentioning

confidence: 71%

“…Finally, These results are comparable with those obtained by flow cytometry of the normal human adult liver, in there was no correlation of loss (or gain) with the histopathological Edmondson grades or with the cause of the which approximately 55% of hepatocytes are diploid, and the remainder are mostly tetraploid. 23,24 Because HCC. However, this series is too small to be conclusive.…”

Section: Fish Resultsmentioning

confidence: 99%

Interphase cytogenetic studies of human hepatocellular carcinomas by fluorescent in situ hybridization

Hamon-Benais

¹

,

Ingster

²

,

Terris

³

et al. 1996

View full text Add to dashboard Cite

Although numerous allelic chromosome losses have been reported in hepatocellular carcinomas (HCC), chromosome analysis by cytogenetic methods has rarely been performed in these tumors, unlike other solid malignant tumors. The purpose of the current study was to analyze primary liver tumors by conventional cytogenetic methods and by a new molecular cytogenetic technique, called fluorescent in situ hybridization (FISH), a technique that has been recently proposed to count the number of chromosome copies in interphase nuclei with chromosome centromeric probes. Primary cultures of tumoral cells were prepared to obtain metaphases. Specific chromosomes probes 7, 17, and 20 were used to perform in situ hybridization on isolated intact tumoral cells. Seven cases of primary liver tumors (six cases of HCC and one case of benign focal hepatic nodular hyperplasia) were investigated. A few metaphases were obtained in five of the seven tumors, and in most cases numerical abnormalities were difficult to interpret. In contrast with in situ hybridization, all cases of HCC showed losses and/or gains of chromosomes. Loss of one to three chromosomes occurred in five tumors. A gain of two chromosomes was observed in two of these five tumors. In only one case, a gain of only three chromosomes occurred. In addition, a loss of chromosome 17 was recorded for the benign tumor. These results demonstrate that FISH with specific probes can provide information on chromosome number in the tumoral cells of primary liver tumors even in the absence of analyzable metaphases. This technique opens new possibilities for the investigation of chromosome abnormalities in HCC.

show abstract

Liver ploidy

Cited by 18 publications

References 23 publications

Interphase cytogenetic studies of human hepatocellular carcinomas by fluorescent in situ hybridization

Interphase cytogenetic studies of human hepatocellular carcinomas by fluorescent in situ hybridization

Anatomy, pathophysiology and basic mechanisms of disease

Interphase cytogenetic studies of human hepatocellular carcinomas by fluorescent in situ hybridization

Contact Info

Product

Resources

About