Liver regeneration in dogs: Morphologic and chemical changes

Francavilla, A.; Porter, Kenneth Wiggins; Benichou, Joseph; Jones, Arthur F.; Starzl, Thomas E.

doi:10.1016/s0022-4804(78)80005-5

Cited by 71 publications

(39 citation statements)

References 20 publications

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“…In dogs, the crude cytosol from the residual liver fragment does not possess stimulatory activity until 48 hr, reaching maximum potency after 72 hr as determined in the canine Eck's fistula model (12) or by the augmentation of hepatic regeneration in a second dog after subtotal hepatectomy (48). The time when hepatic regeneration in dogs reaches its peak is 3 days (49).…”

Section: Discussionmentioning

confidence: 99%

Screening for candidate hepatic growth factors by selective portal infusion after canine Eck's fistula

Francavilla

1991

Hepatology

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Section: Discussionmentioning

confidence: 99%

Screening for candidate hepatic growth factors by selective portal infusion after canine Eck's fistula

Francavilla

1991

Hepatology

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“…Norit A and dextran C were obtained from Fisher Scientific Company, Pittsburgh, Pa. Radioactive (2,4,6,7,16,17) H]R1881), nonradioactive R1881, and Econofluor scintillation fluid were obtained from New England Nuclear, Boston, Mass. Aqueous Counting Scintillant was purchased from Amersham, Arlington Heights, Ill.…”

Section: Methodsmentioning

confidence: 99%

Sex Hormone-Related Functions in Regenerating Male Rat Liver

Francavilla¹,

Eagon²,

DiLeo³

et al. 1986

Gastroenterology

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Sex hormone receptors were quantitated in normal male rat liver and in regenerating liver at several different times after partial (70%) hepatectomy. Both estrogen and androgen receptor content were altered dramatically by partial hepatectomy. Total hepatic content and nuclear retention of estrogen receptors increased, with the zenith evident 2 days after partial hepatectomy, corresponding to the zenith of mitotic index. Serum estradiol increased after 1 day, and reached a maximum at 3 days after surgery. In contrast, total and nuclear androgen receptor content demonstrated a massive decline at 1, 2, and 3 days after resection. Serum testosterone displayed a parallel decline. In addition, hepatic content of two androgen-responsive proteins was reduced to 15% and 13% of normal values during this period. The activity of these various proteins during regeneration of male rat liver is comparable to that observed in the liver of normal female rats. Taken together, these results indicate that partial hepatectomy induces a feminization of certain sexually dimorphic aspects of liver function in male rats. Furthermore, these data provide evidence that estrogens, but not androgens, may have an important role in the process of liver regeneration.Mammalian liver of both sexes is responsive to sex hormones. Liver is estrogen responsive in that it contains receptors for this hormone and responds by the synthesis of specific proteins (reviewed in Reference 1). Liver contains androgen receptors as well (2-5). In fact, the sexually dimorphic hepatic content of certain receptors and microsomal enzymes observed in male rats is maintained by testosterone (reviewed in References 1 and 6). Ultimate control over these sex differences in hepatic function resides in the pituitary gland. In particular, growth hormone secretion patterns, also sexually dimorphic in nature, appear to regulate hepatic levels of many microsomal steroid and drug metabolizing enzymes as well as steroid receptor levels (1,7,8).Since Higgins and Anderson originally reported (9) the technique of partial hepatectomy (PH) in rats, investigators have been studying the effect of hepatic resection and the remnant liver with the goal of identifying the mechanisms that regulate hepatic regeneration. During the last 10 yr, hormones have assumed a prominent role as factors related in some way to hepatic regeneration after PH. It has been suggested that hormonal agents such as insulin, glucagon, and epidermal growth factor may initiate the process of regeneration (10-17); however, other hormones such as parathyroid hormone (18) (23). These data demonstrated that the proliferative activity of the regenerating liver is concurrent with a significant increase in nuclear localization of hepatic ERs. However, this study did not examine possible increases in serum estradiol content that might account for the increased nuclear ER localization, nor was total hepatic content of ER examined. We have now extended the study to answer these questions, and in addition, to determin...

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“…In normal unoperated dogs, the liver weight per body weight percent is 2.43 ± 0.66 (S.D.) per cent (21). Using the reduced final body weight as reference (Table 1), the control animals with pes alone (Groups 1 and 3) had a diminished liver weight per body weight.…”

Section: Pathologic Studiesmentioning

confidence: 99%

Morphologic and Biochemical Changes in Dogs After Portacaval Shunt Plus Bile Fistula or Ileal Bypass: Failure of Bile Fistula or Ileal Bypass to Prevent Hepatocyte Atrophy

et al. 1983

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External biliary fistula (BF) or ileal bypass (IB) was performed in dogs at the time of or 2 weeks after portacaval shunt (PCS). The pathologic changes in the dog livers 2 to 4 weeks later were compared to those caused by PCS alone. Histopathologic differences between PCS alone vs. PCS plus BF or IB could not be found. Thus, the experiments did not confirm recent observations by others in rats that BF prevents or reverses the hepatic injury of PCS. As estimated by plasma mevalonic acid determinations, the increase in hepatic cholesterol synthesis that is characteristic after BF or IB was suppressed in animals with PCS. BF and m reduced but did not eliminate the postprandial elevation in serum bile acid that occurs after PCS. The findings have possible relevance in planning the treatment of patients with familial hypercholesterolemia with the combined use of PCS and lB.Completely diverting portacaval shunt (PCS), also called Eck's fistula, has been used for its effect upon the metabolism of patients with glycogen storage disease (1), familial hypercholesterolemia (2, 3), and aI-antitrypsin deficiency (4). The metabolic palliation in these diseases has been ascribed in part to the hepatocyte alterations caused by portal diversion including atrophy, deglycogentation, fat accumulation, increased mitosis, and major ultrastructural disruptions (2,(4)(5)(6)(7)(8)(9). These changes are identical in rats, dogs, and humans (5). Thus, research with any of these species should be applicable to all in spite of the fact that humans are unusually resistant to the hepatic encephalopathy that may complicate PCS.Balabaud et a1. (10) and Bioulac et a1. (11) have

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Liver regeneration in dogs: Morphologic and chemical changes

Cited by 71 publications

References 20 publications

Screening for candidate hepatic growth factors by selective portal infusion after canine Eck's fistula

Screening for candidate hepatic growth factors by selective portal infusion after canine Eck's fistula

Sex Hormone-Related Functions in Regenerating Male Rat Liver

Morphologic and Biochemical Changes in Dogs After Portacaval Shunt Plus Bile Fistula or Ileal Bypass: Failure of Bile Fistula or Ileal Bypass to Prevent Hepatocyte Atrophy

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