Liver Regeneration in MHC Class I Deficient Mice

Radošević-Stašić, Biserka; Trobonjača, Zlatko; Ćuk, Mira; Ravlić‐Gulan, Jagoda; Rukavina, Daniel

doi:10.1097/00024382-199703001-00264

Cited by 2 publications

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“…Our data therefore support the current knowledge [34–43], emphasizing that during the syngeneic pregnancy the maternal liver is a site of potent cytotoxicity. It is likely that there, hepatic CD1‐restricted and activated NKT cells lyse autologous cells via TCR ligation in the absence of any identifiable cognate Ag, while the specific ‘inflammatory’ environment induces the Ag‐nonspecific cytotoxic functions (LAK activity) of NKT and γδ T cells [42].…”

Section: Discussionsupporting

confidence: 92%

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Syngeneic Pregnancy Induces Overexpression of Natural Killer T Cells in Maternal Liver

et al. 2003

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Conditions such as stress, infection, autoimmune disease, etc. elevate the number and function of extrathymic T cells that are generated mainly in the liver. As primitive, self-reactive clones of T cells that coexpress receptors of the natural killer (NK) lineage, they mediate cytotoxicity against altered self, malignant and infected cells and have the unique potential to rapidly secrete large amount of T helper 1 (Th1) or Th2 cytokines.To elucidate whether some of these changes occur even during the syngeneic pregnancy, we made phenotypic and functional characterization of mononuclear lymphatic cells (MNLCs) isolated from the liver and spleen of pregnant C57BL/ 6 mice, testing their cytotoxicity against syngeneic thymocytes as well as against NK-and lymphokine-activated killer (LAK)-sensitive targets. The data have shown that on the sixteenth day of syngeneic pregnancy TCR int , NK1.1 þ and IL-2Rb þ cells were accumulated in the liver, while the quantities of CD4 þ and CD8þ T cells and total number classical NK (NK1.1 þ CD3 -or IL-2Rb þ CD3 -) cells were increased in the spleen. Pregnancy-activated hepatic and splenic MNLCs were more cytotoxic against syngeneic thymocytes, YAC-1 and P815 targets, suggesting that the maternal liver is a main producer of autoreactive NKT clones, which subsequently augment NK-and LAK cell-mediated cytotoxicity in the liver and spleen.

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Section: Discussionsupporting

confidence: 92%

“…Isolation of hepatic and splenic MNLCs MNLCs were isolated from liver, using a modification of the method of Seglen, as we previously described [43]. For this purpose, in situ slow perfusion with 10 ml of phosphate‐buffered saline (PBS) was used.…”

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Syngeneic Pregnancy Induces Overexpression of Natural Killer T Cells in Maternal Liver

et al. 2003

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“…21 Resident liver MNLC were isolated by Ficoll-Hypaque density gradient centrifugation (20 min at 800 ´g). A single suspension of spleen cells was prepared in RPMI 1640 medium (Gipco BRL, Basel, Switzerland), after elimination of erythrocytes by lysing solution.…”

Section: Isolation Of Intrahepatic Lymphocytes Splenocytes and Thymomentioning

confidence: 99%

Augmentation of NKT and NK cell‐mediated cytotoxicity by peptidoglycan monomer linked with zinc

Mrakovčić-Šutić

Radošević-Stašić

Šimin

et al. 2002

Mediators of Inflammation

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BACKGROUND: Peptidoglycan monomer (PGM), which was originally prepared by biosynthesis from culture fluids of penicillin-treated Brevibacterium divaricatum, is an immunostimulator, the activities of which might be improved by addition of zinc (Zn) to the basic molecule. METHODS: To test the possible cytotoxic effects of this new analogue, we analyzed the ability of PGM-Zn and PGM to change the phenotypic profile of hepatic and splenic mononuclear lymphatic cells and to affect the growth of malignant T-cell line YAC-1 and syngeneic thymocytes. RESULTS: Pretreatment of C57BL/6 mice primarily with PGM-Zn over 6 days (10/mg/kg intraperitoneally) significantly enhanced the proportions of NK1.1high+, CD4-CD8-, CD69+, and CD3intermediate/NK1.1+/IL2R-beta+ (NKT) cells in the liver, and major histocompatibility complex class II+, CD69+, and CD8+ cells in the spleen. Both types of cells were highly cytotoxic against YAC-1 and syngeneic thymocytes, increasing the destruction of YAC-1 by 70% on addition of hepatic cells and by 30% on addition of splenic cells. Destruction of thymocytes increased by 10 and 50%, respectively. CONCLUSION: The results point to PGM-Zn as a potent cytotoxicity-inducing agent, which also generates autoreactive NKT cells.

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Liver Regeneration in MHC Class I Deficient Mice

Cited by 2 publications

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Syngeneic Pregnancy Induces Overexpression of Natural Killer T Cells in Maternal Liver

Syngeneic Pregnancy Induces Overexpression of Natural Killer T Cells in Maternal Liver

Augmentation of NKT and NK cell‐mediated cytotoxicity by peptidoglycan monomer linked with zinc

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