Living cells in 1 of 2 frozen femoral heads

Autogenous skull flaps stored frozen for more than 6 months: do they remain viable?

“…10,11 Similar studies have not previously been performed on human skull bone. In the current study, the authors aimed to assess whether viable bone cells may be cultured from human skull flaps which had been cryopreserved for a similar duration.…”

Donor derived malignancy following transplantation: a review

“…Frozen and freeze-dried are considered safe and it is believed that there are no viable cells in them although some cells may survive in the frozen grafts. For example, recent publications have demonstrated the presence of donor derived living cells in a culture medium from frozen bone grafts (Heyligers and Klein-Nulend 2005;Weyts et al 2003). Although, there are no reports of tumor transmission using bone grafts, a study that evaluated osteoarthritic femoral heads removed during hip arthroplasty and potentially useable as bone grafts, showed 8% evidence of diseases not previously known including malignancy (Palmer et al 1999).…”

Efeito da utilização de plasma rico em plaquetas na osteointegração dos enxertos ósseos homólogos criopreservados: estudo histomorfométrico em coelhos

“…Ainda Consideramos que o modelo adotado não nos permite afirmar se as células observadas na zona de reparo são procedentes do leito receptor ou próprias do aloenxerto criopreservado, como relatado em alguns estudos que destacam a presença de células vivas e com potencial de crescimento no tecido ósseo criopreservados a -80 °C (Weyts et al, 2003;Heyligers, Kleim, 2005). Entretanto, foi evidente que a matriz enxertada serviu de molde para a sobrevivência das células durante a fase inicial da reparação do tecido lesado.…”