Living on the edge: the role of Atgolgin‐84A at the plant ER–Golgi interface

Vieira, Vanessa; Pain, Charlotte; Wojcik, Stefan; Rossi, Tatiana Spatola; Denecke, Jürgen; Osterrieder, Anne; Hawes, Chris; Kriechbaumer, Verena

doi:10.1111/jmi.12946

Cited by 10 publications

(7 citation statements)

References 69 publications

(108 reference statements)

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“…We found that Golgi bodies are motile and associated with ER tubules (Figure 1A; Supplementary movie S1) or curved edges of cisternae (Figure 1B) or may be trapped within small lacunae in ER cisternae (Figure 1C). Although connections between ER and Golgi bodies have been described before (daSilva et al, 2004;Sparkes et al, 2009;Osterrieder et al, 2017;Vieira et al, 2020), our results indicate that we can visualize the spatial distribution of the ER and Golgi in live cells at a level of resolution that has not been reported before.…”

Section: High-resolution Imaging Of the Er-golgi Interface In Live Pl...mentioning

confidence: 49%

“…Pre-cis-Golgi compartments have been previously suggested for proteins proposed to be involved in ER-Golgi tethering: AtGolgin-84A, which colocalizes with Sar1a (Vieira et al, 2020), as well as AtCASP (Latijnhouwers et al, 2007;Osterrieder et al, 2017) and the Arabidopsis ER-a-mannosidase I (MNS3; (Schoberer et al, 2019)). Our results support the existence of a pre-cis-Golgi compartment where COPII components accumulate rather than accumulating on the ER.…”

Section: Discussionmentioning

confidence: 99%

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On the nature of the plant ER exit sites

et al. 2022

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In plants, the endoplasmic reticulum (ER) and Golgi bodies are not only in close proximity, but are also physically linked. This unique organization raises questions about the nature of the transport vectors carrying cargo between the two organelles. Same as in metazoan and yeast cells, it was suggested that cargo is transported from the ER to Golgi cisternae via COPII-coated vesicles produced at ribosome-free ER exit sites (ERES). Recent developments in mammalian cell research suggest, though, that COPII helps to select secretory cargo, but does not coat the carriers leaving the ER. Furthermore, it was shown that mammalian ERES expand into a tubular network containing secretory cargo, but no COPII components. Because of the close association of the ER and Golgi bodies in plant cells, it was previously proposed that ERES and the Golgi comprise a secretory unit that travels over or with a motile ER membrane. In this study, we aimed to explore the nature of ERES in plant cells and took advantage of high-resolution confocal microscopy and imaged ERES labelled with canonical markers (Sar1a, Sec16, Sec24). We found that ERES are dynamically connected to Golgi bodies and most likely represent pre-cis-Golgi cisternae. Furthermore, we showed fine tubular connections from the ER to Golgi compartments (ERGo tubules) as well as fine protrusions from ERES/Golgi cisternae connecting with the ER. We suggest that these tubules observed between the ER and Golgi as well as between the ER and ERES are involved in stabilizing the physical connection between ER and ERES/Golgi cisternae, but may also be involved in cargo transport from the ER to Golgi bodies.

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Section: High-resolution Imaging Of the Er-golgi Interface In Live Pl...mentioning

confidence: 49%

Section: Discussionmentioning

confidence: 99%

On the nature of the plant ER exit sites

et al. 2022

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“…Golgi body distribution was assessed as in Renka 77 and Vieira et al 76 Briefly, images of Golgi body distribution were collected and cropped to the largest possible rectangle containing only cell area within the field of view. Using the ImageJ multi-point tool, the co-ordinates of each Golgi body were manually obtained.…”

Section: Golgi Body Distributionmentioning

confidence: 99%

intER‐ACTINg: The structure and dynamics of ER and actin are interlinked

Pain

Tolmie

Wojcik

et al. 2022

Journal of Microscopy

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The actin cytoskeleton is the driver of gross ER remodelling and the movement and positioning of other membrane-bound organelles such as Golgi bodies. Rapid ER membrane remodelling is a feature of most plant cells and is important for normal cellular processes, including targeted secretion, immunity and signalling. Modifications to the actin cytoskeleton through pharmacological agents such as Latrunculin B and phalloidin, or disruption of normal myosin function also affect ER structure and/or dynamics. Here, we investigate the impact of changes in the actin cytoskeleton on structure and dynamics on the ER as well as in return the impact of modified ER structure on the architecture of the actin cytoskeleton. By expressing actin markers that affect actin dynamics, or expressing of ER-shaping proteins that influence ER architecture, we found that the structure of ER-actin networks is closely inter-related; affecting one component is likely to have a direct effect on the other. Therefore, our results indicate that a complicated regulatory machinery and cross-talk between these two structures must exist in plants to co-ordinate the function of ER-actin network during multiple subcellular processes. In addition, when considering organelle structure and dynamics, the choice of actin marker is essential in preventing off-target organelle structure and dynamics modifications.

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“…Atp115/MAG4 locates at cis -Golgi and mediates the transport of storage protein precursors from the ER to the Golgi ( Takahashi et al, 2010 ). AtCASP and Atgolgin84 locates to the cis -Golgi and are implicated in tethering the Golgi to the ERES ( Latijnhouwers et al, 2007 ; Osterrieder et al, 2017 ; Vieira et al, 2020 ). So far, except for Atp115, no biological study of golgin on plant development has been conducted.…”

Section: Intra-golgi Transportmentioning

confidence: 99%

An Update on the Key Factors Required for Plant Golgi Structure Maintenance

et al. 2022

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Plant Golgi apparatus serves as the central station of the secretory pathway and is the site where protein modification and cell wall matrix polysaccharides synthesis occur. The polarized and stacked cisternal structure is a prerequisite for Golgi function. Our understanding of Golgi structure maintenance and trafficking are largely obtained from mammals and yeast, yet, plant Golgi has many different aspects. In this review, we summarize the key players in Golgi maintenance demonstrated by genetic studies in plants, which function in ER-Golgi, intra-Golgi and post-Golgi transport pathways. Among these, we emphasize on players in intra-Golgi trafficking.

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Living on the edge: the role of Atgolgin‐84A at the plant ER–Golgi interface

Cited by 10 publications

References 69 publications

On the nature of the plant ER exit sites

On the nature of the plant ER exit sites

intER‐ACTINg: The structure and dynamics of ER and actin are interlinked

An Update on the Key Factors Required for Plant Golgi Structure Maintenance

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