Investigating organizational transformation in supply chains: A case study on B2B and Extranet impact Organizations introduce e-business applications as a tool for seamless, streamlined and integrated supply chain operations. However, the findings underline that managers not only must recognize differences between individual e-business applications but also their impact upon their supply chains in order to avoid pushing one-size transformation efforts throughout their supply chains. This paper examines the impact of B2B and Extranet on one automotive supply chain and investigates its transformation context. A case study including a participant observation in an automotive supply chain has been used. The impact of B2B and Extranet has been evaluated based on eight dimensions of transformation. Consistent with the organizational transformation literature, the empirical results of transformation indicated that organizational transformation elements content, infrastructure, strategic intent are integral to transformation process in supply chains in terms of B2B and Extranet. However, same supply chain members may experience different patterns of impact based on each ebusiness application. The differences in this case study were observed in following organizational transformation dimensions; competencies, resources, challenges and strategic response. The present work is exploratory in nature. Further research is needed to validate and substantiate the findings in other supply chains.