LKB1/AMPK pathway mediates resistin-induced cardiomyocyte hypertrophy in H9c2 embryonic rat cardiomyocytes

Abstract

Abstract.Resistin has been previously demonstrated to induce cardiac hypertrophy, however, the underlying molecular mechanisms of resistin-induced cardiac hypertrophy remain unclear. Using H9c2 cells, the present study investigated the liver kinase B1 (LKB1)/adenosine monophosphate-activated protein kinase (AMPK) signaling pathway for a potential role in mediating resistin-induced cardiomyocyte hypertrophy. Treatment of H9c2 cells with resistin increased cell surface area, protein synthesis, and expression of hypertrophic marker brain natriuretic peptide and β-myosin heavy chain. Treatment with metformine attenuated these effects of resistin. Furthermore, treatment with resistin decreased phosphorylation of LKB1 and AMPK, whereas pretreatment with metformin increased phosphorylation of LKB1 and AMPK that is reduced by resistin. These results suggest that resistin induces cardiac hypertrophy through the inactivation of the LKB1/AMPK cell signaling pathway.

Odanacatib Inhibits Resistin-induced Hypertrophic H9c2 Cardiomyoblast Cells Through LKB1/AMPK Pathway

“…Cells were seeded into a 3.5 cm 2 culture dish at a density of 1x10 5 . Cells were cultured overnight, and then replaced the medium with serumfree medium for 24 h. Cells were treated with resistin at a concentration of 50 ng/ml 18 and ODN at a concentration of 300 nM 19 for the indicated time.…”

“…Cells were cultured with serum-free DMEM for 24 h. Cells were treated with resistin for 48 h or treated with ODN for 2 h and then replaced it with resistin for 46 h. The cell surface area was measured with ImageJ software (NIH ImageJ version 1.49 software, Bethesda, MD, USA). Five observation fields were selected at random and 10 cells in each observation field were selected for measurement of cell surface area 18 .…”

“…Protein concentrations were detected by BCA protein quantitative kit (Boster, Wuhan, China). Cell protein synthesis was determined by dividing the total amount of protein by the cell number 18 .…”

Resistin-induced cardiomyocyte hypertrophy is inhibited by apelin through the inactivation of extracellular signal-regulated kinase signaling pathway in H9c2 embryonic rat cardiomyocytes

“…Cells were cultured at 37˚C with serum-free medium for 18 h and pretreated with 100 nM apelin for 2 h, then treated with 50 ng/ml resistin for 48 h. The cell surface area was measured using ImageJ version 1.49 software (National Institutes of Health, Bethesda, MD, USA). Using an inverted microscope, five observation fields of cells were selected at random and 10 of the cells in each observation field were selected for measurement of their cell surface areas (24).…”

“…Protein concentrations were measured using a bicinchoninic acid (BCA) protein assay kit (Bio-Rad Laboratories, Inc., Hercules, CA, USA) according to the manufacturer's instructions. Cell protein synthesis was expressed as the relative protein content, which was determined by dividing the total protein quantity by the cell number (24).…”