LKTA and PlpE small fragments fusion protein protect against Mannheimia haemolytica challenge

Guzmán-Brambila, Carolina; Quintero-Fabián, Saray; González-Castillo, Celia; Valle, Álvaro de Obeso-Fernández del; Flores-Samaniego, Beatriz; Mora, Germán de la; Rojas-Mayorquín, Argelia E.; Ortuño-Sahagún, Daniel

doi:10.1016/j.rvsc.2012.07.004

Cited by 8 publications

(7 citation statements)

References 31 publications

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“…Some of the outer membrane proteins identified in this work have been reported as important immunogens and have been evaluated as recombinant subunit antigens due to their protective vaccine potential ( Ayalew et al, 2011 ; Guzman-Brambila et al, 2012 ). In particular, vaccination of mice and cattle with recombinant serotype specific antigen 1 (rSsa1) stimulated high antibody responses ( Ayalew et al, 2011 ).…”

Section: Discussionmentioning

confidence: 99%

Identification of Leukotoxin and other vaccine candidate proteins in a Mannheimia haemolytica commercial antigen

Tucci

Estevez²,

Becco³

et al. 2016

Heliyon

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Bovine Respiratory Disease is the most costly disease that affects beef and dairy cattle industry. Its etiology is multifactorial, arising from predisposing environmental stress conditions as well as the action of several different respiratory pathogens. This situation has hindered the development of effective control strategies. Although different type of vaccines are available, many currently marketed vaccines are based on inactivated cultures of the main viral and bacterial agents involved in this pathology. The molecular composition of commercial veterinary vaccines is a critical issue. The present work aims to define at the proteomic level the most relevant valence of a line of commercial respiratory vaccines widely used in Central and South America. Since Mannheimia haemolytica is responsible for most of the disease associated morbid-mortality, we focused on the main proteins secreted by this pathogen, in particular Leukotoxin A, its main virulence factor. By Western blot analysis and mass spectrometry, Leukotoxin A was identified as a major component of M. haemolytica culture supernatants. We also identified other ten M. haemolytica proteins, including outer membrane proteins, periplasmic transmembrane solute transporters and iron binding proteins, which are relevant to achieve protective immunity against the pathogen. This work allowed a detailed molecular characterization of this vaccine component, providing evidence of its quality and efficacy. Furthermore, our results contributed to the identification of several proteins of interest as subunit vaccine candidates.

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Section: Discussionmentioning

confidence: 99%

Identification of Leukotoxin and other vaccine candidate proteins in a Mannheimia haemolytica commercial antigen

Tucci

Estevez²,

Becco³

et al. 2016

Heliyon

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“…The ability of H. somni to resist leukocytes while creating a proinflammatory and procoagulation environment at the endothelial cell surface and the ability of M. haemolytica to circumvent leukocyte antibacterial activity via its leukotoxin LKTA probably contribute to the intense inflammation that characterizes BRD (10). As such, it seems feasible to propose that a vaccine that combines antigenic surface proteins from M. haemolytica (e.g., different fragments of LktA and Plp) (7,22) and H. somni (e.g., p31 and p40, as demonstrated here) may be useful in preventing infection and reducing the incidence of BRD. Further experiments would be needed to test this hypothesis.…”

Section: Discussionmentioning

confidence: 99%

“…We previously formulated an experimental vaccine using two fragments from Mannheimia haemolytica OMPs that elicited high antibody titers in rabbits and sheep and that protected mice against bacterial challenge (22). However, since BRD is a complex pathology a multivalent vaccine is more desirable, both to increase the protective range and to simplify the vaccination schedule.…”

mentioning

confidence: 99%

Two Outer Membrane Lipoproteins from Histophilus somni Are Immunogenic in Rabbits and Sheep and Induce Protection against Bacterial Challenge in Mice

Guzmán-Brambila¹,

Rojas-Mayorquín²,

Flores-Samaniego³

et al. 2012

Clin. Vaccine Immunol.

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dHistophilus somni is an economically important pathogen of cattle and other ruminants and is considered one of the key components of the bovine respiratory disease (BRD) complex, the leading cause of economic loss in the livestock industry. BRD is a multifactorial syndrome, in which a triad of agents, including bacteria, viruses, and predisposing factors or "stressors," combines to induce disease. Although vaccines against H. somni have been used for many decades, traditional bacterins have failed to demonstrate effective protection in vaccinated animals. Hence, the BRD complex continues to produce strong adverse effects on the health and well-being of stock and feeder cattle. The generation of recombinant proteins may facilitate the development of more effective vaccines against H. somni, which could confer better protection against BRD. In the present study, primers were designed to amplify, clone, express, and purify two recombinant lipoproteins from H. somni, p31 (Plp4) and p40 (LppB), which are structural proteins of the outer bacterial membrane. The results presented here demonstrate, to our knowledge for the first time, that when formulated, an experimental vaccine enriched with these two recombinant lipoproteins generates high antibody titers in rabbits and sheep and exerts a protective effect in mice against septicemia induced by H. somni bacterial challenge.

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“…BCoV generally causes respiratory and enteric infections in cattle and wild ruminants, being the cause of three different syndromes (Saif, 2010): enteric disease with diarrhea in calves, winter dysentery with hemorrhagic diarrhea in adults (especially in lactating cows, and respiratory infections in cattle with a wide age range in calves), and the complex called bovine respiratory disease or shipping fever in beef cattle (Clark, 1993), in which bacterial infections coexist with viral infection (Guzmán-Brambila, et al, 2012).…”

Section: Bovine Coronavirusmentioning

confidence: 99%

“…El BCoV causa generalmente infecciones respiratorias y entéricas en bovinos y rumiantes salvajes, al ser la causa de tres síndromes distintos (Saif, 2010): la enfermedad entérica con diarrea en terneros, la disentería de invierno con diarrea hemorrágica en adultos (especialmente en vacas lactantes, e infecciones respiratorias en bovinos con un amplio intervalo de edad en los terneros) y el complejo denominado enfermedad respiratoria bovina o la fiebre de embarque en el ganado de engorda (Clark, 1993), en la que coexisten infecciones bacterianas con la infección viral (Guzmán-Brambila, et al, 2012).…”

Section: Coronavirus Bovinounclassified

Coronavirosis: signos y síntomas con un enfoque en SARS CoV-2. Una revisión comparativa entre especies.

Esparza

Ortuño-Sahagún

2020

rbio

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La enfermedad de la COVID-19 afecta a humanos y es causada por el coronavirus SARS CoV-2. Es una afección emergente que se caracteriza por causar fiebre, malestar, fatiga, dificultad respiratoria, tos seca, diarrea y dolor de cabeza; en los casos más graves puede ocasionar neumonía severa, ocasionalmente conjuntivitis y erupciones cutáneas. Recientemente se ha demostrado que SARS CoV- 2 es capaz de infectar otras especies y estas pueden presentar signos similares a los de COVID-19. Esta revisión hace una comparación de los signos y síntomas de las coronavirosis que afectan a las principales especies domésticas. Adicionalmente, presenta una recopilación de reportes zoosanitarios y trabajos experimentales de la susceptibilidad de animales ante la infección de SARS CoV-2, ya sea por contacto con personas positivas a SARS CoV-2 o por inoculación experimental. Finalmente, se comparan los signos en animales con los síntomas de la enfermedad de la COVID-19 y se demuestra que los animales filogenéticamente más cercanos al humano fueron también los más susceptibles.La enfermedad de la COVID-19 afecta a humanos y es causada por el coronavirus SARS CoV-2. Es una afección emergente que se caracteriza por causar fiebre, malestar, fatiga, dificultad respiratoria, tos seca, diarrea y dolor de cabeza,; en los casos más graves puede ocasionar neumonía severa, ocasionalmente conjuntivitis y erupciones cutáneas. Recientemente se ha demostrado que SARS CoV- 2 es capaz de infectar otras especies y estas pueden presentar signos similares a los de COVID-19. Esta revisión hace una comparación de los signos y síntomas de las coronavirosis que afectan a las principales especies domésticas. Adicionalmente, presenta una recopilación de reportes zoosanitarios y trabajos experimentales de la susceptibilidad de animales ante la infección de SARS CoV-2, ya sea por contacto con personas positivas a SARS CoV-2 o por inoculación experimental. Finalmente, se comparan los signos en animales con los síntomas de la enfermedad de la COVID-19 y se demostró que los animales filogenéticamente más cercanos al humano fueron también los más susceptibles.

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LKTA and PlpE small fragments fusion protein protect against Mannheimia haemolytica challenge

Cited by 8 publications

References 31 publications

Identification of Leukotoxin and other vaccine candidate proteins in a Mannheimia haemolytica commercial antigen

Identification of Leukotoxin and other vaccine candidate proteins in a Mannheimia haemolytica commercial antigen

Two Outer Membrane Lipoproteins from Histophilus somni Are Immunogenic in Rabbits and Sheep and Induce Protection against Bacterial Challenge in Mice

Coronavirosis: signos y síntomas con un enfoque en SARS CoV-2. Una revisión comparativa entre especies.

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