LmCht5-1 promotes pro-nymphal molting during locust embryonic development

Zhang, Tingting; Liu, Weiwei; Li, Daqi; Ma, Enbo; Zhu, Kun Yan; Moussian, Bernard; Li, Sheng; Zhang, Jianzhen

doi:10.1016/j.ibmb.2018.09.001

Cited by 23 publications

(31 citation statements)

References 21 publications

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“…After silencing the two genes, only LmCht5-1 was found to cause a lethal phenotype and high mortality during nymph-adult transition (Li et al, 2015), which is a finding that is consistent with our results in this S. furcifera Cht5 study. Zhang et al (2018) found LmCht5-1 transcript levels to be high in eggs, and dsLmCht5-1 injection into 8-day-old embryos resulted in eggs failing to hatch into 1st-instar nymphs. Here, because we found that SfCht5 was highly expressed in the egg stage, we hypothesized that SfCht5 is involved in egg hatching.…”

Section: Discussionmentioning

confidence: 94%

Characterization and functional analysis of chitinase family genes involved in nymph–adult transition of Sogatella furcifera

et al. 2020

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Chitinase degrades chitin in the old epidermis or peritrophic matrix of insects, which ensures normal development and metamorphosis. In our previous work, we comprehensively studied the function of SfCht7 in Sogatella furcifera. However, the number and function of chitinase genes in S. furcifera remain unknown. Here, we identified 12 full-length chitinase transcripts from S. furcifera, which included nine chitinase (Cht), two imaginal disc growth factor (IDGF), and one endo-β-N-acetylglucosaminidase (EN-Gase) genes. Expression analysis results revealed that the expression levels of eight genes (SfCht3,

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Section: Discussionmentioning

confidence: 94%

Characterization and functional analysis of chitinase family genes involved in nymph–adult transition of Sogatella furcifera

et al. 2020

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“…Chitinase 5 [Cht5]) are postulated to catalyze old cuticle degradation at apolysis (detachment of the old cuticle) during molting (Zhu et al ., 2008; Wu et al ., 2013; Xi et al ., 2015; Pesch et al ., 2016). Consistently, in Locusta migratoria , LmCht5‐1 plays an important role in lamellar procuticle degradation in nymphs and in the embryo (Li et al ., 2015; Li et al ., 2016; Zhang et al ., 2018). Unlike group I enzymes with a single catalytic domain, group II chitinases have multiple catalytic and chitin‐binding domains.…”

Section: Introductionmentioning

confidence: 99%

Chitinase 10 controls chitin amounts and organization in the wing cuticle of Drosophila

et al. 2020

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Wings are essential for insect fitness. A number of proteins and enzymes have been identified to be involved in wing terminal differentiation, which is characterized by the formation of the wing cuticle. Here, we addressed the question whether chitinase 10 (Cht10) may play an important role in chitin organization in the wings of the fruit fly Drosophila melanogaster. Initially, we first found that Cht10 expression coincides with the expression of the chitin synthase coding gene kkv. This suggests that the respective proteins may cooperate during wing differentiation. In tissue-specific RNA interference experiments, we demonstrate that suppression of Cht10 causes an excess in chitin amounts in the wing cuticle. Chitin organization is severely disrupted in these wings. Based on these data, we hypothesize that Cht10 restricts chitin amounts produced by Kkv in order to ensure normal chitin organization and wing cuticle formation. In addition, we found by scanning electron microscopy that Cht10 suppression also affects the cuticle surface. In turn, cuticle inward permeability is enhanced in Cht10-less wings. Moreover, flies with reduced Cht10 function are unable to fly. In conclusion, Cht10 is essential for wing terminal differentiation and function.

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“…Knocking down Cht5 , −6 and −8 resulted in delayed development and high mortality in Chilo suppressalis Walker . In Locusta migratoria , LmCht5‐1 is highly expressed to promote pro‐nymphal molting during embryonic development, and in B. mori , BmCht5 expression follows a circadian rhythm to participate in hatching behavior . In the present study, high‐throughput sequencing revealed that different expression patterns of Cht genes, including Cht‐2 , −5 , −7 , −8 and −10 , were involved in regulating different stages of development and metamorphosis.…”

Section: Discussionmentioning

confidence: 55%

Potential targets for controlling Bactrocera dorsalis using cuticle‐ and hormone‐related genes revealed by a developmental transcriptome analysis

Liu

Xia

Zhang

et al. 2020

Pest Management Science

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BACKGROUND: The oriental fruit fly, Bactrocera dorsalis (Hendel), is an important agricultural pest and has developed resistance to many insecticides. To investigate vital genes participating in metamorphosis for development of additional control strategies, a comprehensive transcriptome analysis covering ten developmental stages of B. dorsalis was performed.RESULTS: There were 2132, 952, 1062, 2301 and 1333 differentially expressed genes identified during hatching, 1st-instar larval molting, 2nd-instar larval molting, pupariation and emergence, respectively. Further expression analyses indicated that genes in hormone-(20-hydroxyecdysone and juvenile hormone) and cuticle-(chitin and cuticle protein) related pathways were essential for metamorphosis in B. dorsalis. Among chitinase (Cht) genes, BdCht-5, −8 and −10 were differentially expressed during larval-larval, larval-pupal and pupal-adult moltings. However, BdCht7 was differentially expressed during egg-larval and larval-larval moltings. Knockdown of BdCht7 at the 1st-instar larval stage disrupted normal development of larvae and was lethal to B. dorsalis. Among cuticle protein (CP) genes, 15 genes (BdCPLCG-1, and BdRR1-41) were differentially expressed during four of five types of moltings. Among hormone-relative genes, BdJHBP-4, −9 and −13 were differentially expressed during all five types of moltings, whereas BdJHBP-5, −12 and BdHR4 were differentially expressed during four of five types of moltings.CONCLUSION: This study reveals critical genes involved in development and metamorphosis of B. dorsaslis, and BdCht7 is dispensable for larval survival. It also provides comprehensive transcriptome information for finding more molecular targets to control this pest.

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LmCht5-1 promotes pro-nymphal molting during locust embryonic development

Cited by 23 publications

References 21 publications

Characterization and functional analysis of chitinase family genes involved in nymph–adult transition of Sogatella furcifera

Characterization and functional analysis of chitinase family genes involved in nymph–adult transition of Sogatella furcifera

Chitinase 10 controls chitin amounts and organization in the wing cuticle of Drosophila

Potential targets for controlling Bactrocera dorsalis using cuticle‐ and hormone‐related genes revealed by a developmental transcriptome analysis

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