lmmuno Histochemical Profile of Endometrium in Patients With Genital Endometriosis

Abstract

The aim of present study was to investigate the occurence of different lymphocyte subsets in the endometrium of endometriosis patients and in healthy women on every day of the menstrual cycle with special emphasis to the proliferative activity of endometrial cells with Ki-S3 antibody. We also conducted immunohistochemical studies of T-lymphocytes, B-lympho-cytes, macrophages, natural-killer-cells and also of antigens class II of the histocompatibility complex (HLA-DR) during the different phases of the menstrual cycle in endometriosis and non-endometriosis patients. Endometrial lymphocyte subsets show equal quantity and distribution in endometriosis patients and in the control group. After a peak in the early preoliferative phase the absolute number of T-lymphocytes decreases while a predominance of T-suppressor/cytotoxic T-lymphocytes (CD8) compared to T-helper/inducer lymphocytes (CD4) occurs towards the end of the menstrual cycle. It can be concluded that endometrium as the potential parent epithelia of endometriotic lesions seems not to be altered in its lymphatic cell content compared to healthy women. Furthermore endometrium is clearly characterised as part of the mucosa associated lymphatic tissue (MALT). T-lymphocytes show specific quantitative changes due to different phases of the menstrual cycle.

B Cell Subset Analysis and Gene Expression Characterization in Mid-Luteal Endometrium

“…Additionally, the infiltration of plasma cells has been described in endometrial inflammatory conditions such as endometritis (Cicinelli et al, 2019;Song et al, 2019;Zargar et al, 2020), there is a possibility that these GC B cells and Tfh-like cells are implicated in this process by providing the site of plasma cell generation. This proposed GC-like reaction could be linked to the LAs structure identified in the endometrial lower functional layer or the basal layer, where B cells are surrounded by T cells and macrophages (Klentzeris et al, 1992;Mettler et al, 1997;Yeaman et al, 1997Yeaman et al, , 2001Disep et al, 2004). Further research is needed in understanding the cellular components and function of endometrial LAs and whether they can be classified as novel TLSs, and if they alter in endometrial associated reproductive pathologies.…”

The role of endometrial B cells in normal endometrium and benign female reproductive pathologies: a systematic review

“…Evidence of LA-associated B cells in the normal endometrium was found in the control groups of case–control studies ( Klentzeris et al , 1994 , 1995 ; Fernández-Shaw et al , 1995 ; Mettler et al , 1997 ; Disep et al , 2004 ; Kitaya and Yasuo, 2010a ). Apart from B cells (identified by CD20 or CD22), these aggregates also consist of other CD45 + cells, including CD3 + T cells, CD4 + T cells, CD68 + macrophages and CD57 + NK cells, with B cells in the central area and T cells and macrophages in the margin areas ( Klentzeris et al , 1992 ; Mettler et al , 1997 ; Disep et al , 2004 ). LAs were shown to be comprised of several hundred cells, express the endometrial proliferation marker Ki-67 (examined by Ki-S3 antibody) and were mostly HLA-DR + ( Mettler et al , 1997 ).…”

“…Apart from B cells (identified by CD20 or CD22), these aggregates also consist of other CD45 + cells, including CD3 + T cells, CD4 + T cells, CD68 + macrophages and CD57 + NK cells, with B cells in the central area and T cells and macrophages in the margin areas ( Klentzeris et al , 1992 ; Mettler et al , 1997 ; Disep et al , 2004 ). LAs were shown to be comprised of several hundred cells, express the endometrial proliferation marker Ki-67 (examined by Ki-S3 antibody) and were mostly HLA-DR + ( Mettler et al , 1997 ). They were situated in the lower functional layer, or the basal layer, and were observed throughout the menstrual cycle ( Fernández-Shaw et al , 1995 ; Mettler et al , 1997 ).…”

“…LAs were shown to be comprised of several hundred cells, express the endometrial proliferation marker Ki-67 (examined by Ki-S3 antibody) and were mostly HLA-DR + ( Mettler et al , 1997 ). They were situated in the lower functional layer, or the basal layer, and were observed throughout the menstrual cycle ( Fernández-Shaw et al , 1995 ; Mettler et al , 1997 ). Additionally, LAs appeared to have a cyclical influence and were more likely to appear in the follicular phase of the menstrual cycle ( Mettler et al , 1997 ).…”

“…They were situated in the lower functional layer, or the basal layer, and were observed throughout the menstrual cycle ( Fernández-Shaw et al , 1995 ; Mettler et al , 1997 ). Additionally, LAs appeared to have a cyclical influence and were more likely to appear in the follicular phase of the menstrual cycle ( Mettler et al , 1997 ). Additionally, IgA was found mainly at or adjacent to the columnar endometrium epithelium ( Burgener et al , 2013 ).…”