lnc<scp>RNA</scp> H19/miR‐675 axis represses prostate cancer metastasis by targeting <scp>TGFBI</scp>

Zhu, Miaojun; Chen, Qin; Liu, Xin; Sun, Qian; Zhao, Xian; Deng, Rong; Wang, Yanli; Huang, Jian; Xu, Ming; Yan, Jing; Yu, Jianxiu

doi:10.1111/febs.12902

Cited by 271 publications

(217 citation statements)

References 39 publications

Supporting

Mentioning

212

Contrasting

Order By: Relevance

“…Many studies using cell culture systems and mouse models have shown that H19 can function either as an oncogene [25][26][27] or a tumor suppressor. 28,29 Recently, H19 RNA transcripts have been detected in a majority of patientderived premalignant lesions of cervical cancer. 21 Whether H19 is actively involved in the development of cervical cancer and how it works remains to be determined.…”

Section: Introductionmentioning

confidence: 99%

Long non-coding RNA H19 enhances cell proliferation and anchorage-independent growth of cervical cancer cell lines

Iempridee

2016

Exp Biol Med (Maywood)

View full text Add to dashboard Cite

Long non-coding RNA H19 is aberrantly expressed in multiple malignancies and its expression levels correlate with recurrence, metastasis, and patient survival. Despite numerous reports documenting the role of H19 in carcinogenesis, its contribution to cervical cancer development is still largely unknown. In this study, I observed that H19 expression was elevated in cervical cancer cell lines and could be detected in extracellular vesicles in the culture medium. In addition, I demonstrated, by overexpression and knockdown experiments, that H19 promoted cell proliferation and multicellular tumor spheroid formation without significantly affecting apoptosis and cell migration. Finally, treatment with transforming growth factor beta and hypoxia-mimetic CoCl 2 could modulate H19 levels in a cell line-specific manner. These findings indicate that H19 promotes both anchorage-specific andindependent growth of cervical cancer cell lines and may serve as a potential target for cancer diagnosis and therapy.

show abstract

Section: Introductionmentioning

confidence: 99%

Long non-coding RNA H19 enhances cell proliferation and anchorage-independent growth of cervical cancer cell lines

Iempridee

2016

Exp Biol Med (Maywood)

View full text Add to dashboard Cite

show abstract

“…H19 is highly expressed during embryogenesis and is strongly downregulated after birth (23,24). Emerging evidence also indicates that the increased expression of H19 is commonly detected in a broad spectrum of pathological conditions, such as various malignancies (25)(26)(27), and after estrogen treatment (28) or exposure to hypoxia (29). H19 represses embryonic placental growth and trans regulates a network of imprinted genes during fetal development (23,30), but its role in cancer development can be tumor suppressive or oncogenic, depending on cellular content and tumor type (25,29,31).…”

mentioning

confidence: 99%

H19 Long Noncoding RNA Regulates Intestinal Epithelial Barrier Function via MicroRNA 675 by Interacting with RNA-Binding Protein HuR

Zou

Jaladanki

Liu

et al. 2016

Molecular and Cellular Biology

134

118

View full text Add to dashboard Cite

The disruption of the intestinal epithelial barrier function occurs commonly in various pathologies, but the exact mechanisms responsible are unclear. The H19 long noncoding RNA (lncRNA) regulates the expression of different genes and has been implicated in human genetic disorders and cancer. Here, we report that H19 plays an important role in controlling the intestinal epithelial barrier function by serving as a precursor for microRNA 675 (miR-675). H19 overexpression increased the cellular abundance of miR-675, which in turn destabilized and repressed the translation of mRNAs encoding tight junction protein ZO-1 and adherens junction E-cadherin, resulting in the dysfunction of the epithelial barrier. Increasing the level of the RNA-binding protein HuR in cells overexpressing H19 prevented the stimulation of miR-675 processing from H19, promoted ZO-1 and E-cadherin expression, and restored the epithelial barrier function to a nearly normal level. In contrast, the targeted deletion of HuR in intestinal epithelial cells enhanced miR-675 production in the mucosa and delayed the recovery of the gut barrier function after exposure to mesenteric ischemia/reperfusion. These results indicate that H19 interacts with HuR and regulates the intestinal epithelial barrier function via the H19-encoded miR-675 by altering ZO-1 and E-cadherin expression posttranscriptionally.T he majority of the mammalian genome is transcribed into a vast number of noncoding RNAs, whereas protein-coding transcripts account for only a minority of transcriptional output (1, 2). Long noncoding RNAs (lncRNAs) are defined as transcribed RNAs spanning Ͼ200 nucleotides in length that lack protein-coding capacity and are distinct from well-characterized structural RNAs (rRNAs, tRNAs, snRNAs, and snoRNAs) or small regulatory RNAs (3, 4). lncRNAs arise from intergenic, antisense, or promoter-proximal regions, and they share many features with mRNAs. Both classes of RNA can be transcribed from multiexonic genes and possess a 5=-methyl-guanosine cap and 3=-poly(A) tail (1,3,5). Some lncRNAs are ubiquitous, but others are dynamically expressed in tissue-, differentiation stage-, and cell type-specific patterns (4, 6). lncRNAs have been involved in a variety of cellular functions, physiologic processes, and disease states by modulating gene expression at different levels, including chromatin remodeling, transcriptional and posttranscriptional control, and protein metabolism (1, 3, 4). lncRNAs can serve as repressors or activators of gene transcription, as has been extensively reported (1, 4), but lncRNAs also can regulate mRNA decay and translation, working jointly with microRNAs (miRNAs) and RNA-binding proteins (RBPs) (7-9).Intercellular junction complexes, comprising tight junctions (TJs) and adherens junctions (AJs), fence the paracellular space in simple epithelia, such as those lining the intestine, kidneys, and lung, and form an important barrier against a wide array of noxious substances present in the lumen (10, 11). In the intestine, the disruption...

show abstract

“…Previous studies have demonstrated the important roles that lncRNAs serve in cancer tissues (16)(17)(18). Although dysregulated lncRNAs have been implicated in numerous types of cancer, the specific mechanisms underlying their role in these pathologies has yet to be elucidated.…”

Section: Discussionmentioning

confidence: 99%

Long non-coding RNA Igf2as controls hepatocellular carcinoma progression through the ERK/MAPK signaling pathway

et al. 2017

View full text Add to dashboard Cite

Abstract. Long non-coding RNAs (lncRNAs) serve an important role in numerous human diseases, including cancer. Abnormal expression of lncRNAs has been associated with a number of tumor types; however, the underlying mechanisms through which lncRNA functions have yet to be elucidated. The present study primarily focuses on insulin-like growth factor 2 antisense 1 (Igf2as), a lncRNA reported to be differentially expressed in hepatocellular carcinoma (HCC). Reverse transcription-quantitative polymerase chain reaction analysis was used to determine the level of Igf2as in HCC cells and tissues. Flow cytometry was used to determine the level of cell apoptosis following Igf2as suppression and western blot analysis was used to identify altered protein expression levels. The results demonstrated that Igf2as was upregulated in HCC cells and tissues, and that the inhibition of Igf2as using a targeted small interfering RNA (si-Igf2as), significantly decreased cell proliferation and increased apoptosis. Western blot analysis identified that the extracellular signal-regulated kinase/mitogen-activated protein kinase (ERK/MAPK) signaling pathway was inhibited in cells transfected with si-Igf2as. In addition, cell migration was markedly reduced by the knockdown of Igf2as. These results suggest that lncRNA Igf2as may control hepatocellular progression primarily through the regulation of the ERK/ MAPK signaling pathway.

show abstract

lncRNA H19/miR‐675 axis represses prostate cancer metastasis by targeting TGFBI

Cited by 271 publications

References 39 publications

Long non-coding RNA H19 enhances cell proliferation and anchorage-independent growth of cervical cancer cell lines

Long non-coding RNA H19 enhances cell proliferation and anchorage-independent growth of cervical cancer cell lines

H19 Long Noncoding RNA Regulates Intestinal Epithelial Barrier Function via MicroRNA 675 by Interacting with RNA-Binding Protein HuR

Long non-coding RNA Igf2as controls hepatocellular carcinoma progression through the ERK/MAPK signaling pathway

Contact Info

Product

Resources

About