LncRNA AB073614 induces epithelial- mesenchymal transition of colorectal cancer cells via regulating the JAK/STAT3 pathway

Xue, Jinfang; Liao, Liya; Yin, Fei; Kuang, Haoyu; Zhou, Xiaojun; Wang, Yanan

doi:10.3233/cbm-170780

Cited by 38 publications

(34 citation statements)

References 29 publications

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“…28,29 Meanwhile, STAT3 is a significant member of STAT family, which plays a critical role. 32 F I G U R E 4 Effects of LINC00346 on HCC cell migration and invasion. For example, the lncRNA AB073614 induces colorectal cancer EMT via activating the JAK/STAT3 pathway.…”

Section: Discussionmentioning

confidence: 99%

LINC00346 promotes hepatocellular carcinoma progression via activating the JAK‐STAT3 signaling pathway

Yin

Zheng

Zhang

et al. 2019

J of Cellular Biochemistry

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Hepatocellular carcinoma (HCC) remains the most common malignant tumor worldwide. Long noncoding RNAs can modulate various tumorigenic processes. In addition, growing evidence has indicated tha the Janus kinase–signal transducer and activator of transcription (JAK‐STAT) pathway is activated in multiple cancers, including HCC. Recently, it was found that LINC00346 can participate in several cancers. Nevertheless, the biological roles of LINC00346 in HCC have been barely investigated. In this study, the function of LINC00346 was specifically concentrated upon. We observed that LINC00346 was obviously elevated in HCC cells (Bel7404, Huh‐6, HepG2, and QGY‐7703 cells). Then, Bel7404 and HepG2 cells were overexpressed with LINC00346. Overexpression of LINC00346 repressed HCC cell survival and cell proliferation. In addition, apoptosis of Bel7404 and HepG2 cells was triggered by LINC00346 upregulation. Bel7404 and HepG2 cell cycle was arrested in the G1 phase by LINC00346. Meanwhile, we conducted wound‐healing assay and Transwell invasion assays. As shown, we observed that the migratory and invasive capacities of Bel7404 and HepG2 cells were remarkably restrained by the increase of LINC00346. Moreover, we showed that LINC00346 overexpression activated the JAK‐STAT3 pathway, which is involved in many cancers. Afterward, in vivo experiments were utilized and we proved that LINC00346 was able to induce HCC tumor growth via activating the JAK‐STAT3 pathway. To conclude, we revealed the potential possibility of developing LINC00346 as an indicator for HCC.

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Section: Discussionmentioning

confidence: 99%

LINC00346 promotes hepatocellular carcinoma progression via activating the JAK‐STAT3 signaling pathway

Yin

Zheng

Zhang

et al. 2019

J of Cellular Biochemistry

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“…LncRNA play important roles in tumor progression and may be biomarkers for clinical diagnosis and prognosis according to recent studies. For example, in colorectal cancer, the lncRNA AB073614 induced epithelial mesenchymal transition . In cervical cancer, lncRNA SNHG20 promoted cell proliferation and invasion …”

Section: Discussionmentioning

confidence: 99%

“…For example, in colorectal cancer, the lncRNA AB073614 induced epithelial mesenchymal transition. 30 In cervical cancer, lncRNA SNHG20 promoted cell proliferation and invasion. 31 Furthermore, lncRNA were able to compete with mRNA for the binding sites of miRNA which affect the expression of mRNA through MRE.…”

Section: Discussionmentioning

confidence: 99%

Integrated analysis of long noncoding RNA associated‐competing endogenous RNA as prognostic biomarkers in clear cell renal carcinoma

et al. 2018

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Clear cell renal cell carcinoma (ccRCC) is one of the most common malignant carcinomas and its molecular mechanisms remain unclear. Long noncoding RNA (lncRNA) could bind sites of miRNA which affect the expression of mRNA according to the competing endogenous (ceRNA) theory. The aim of the present study was to construct a ceRNA network and to identify key lncRNA to predict survival prognosis. We identified differentially expressed mRNA, lncRNA and miRNA between tumor tissues and normal tissues from The Cancer Genome Atlas database. Then, using bioinformatics tools, we explored the connection of 89 lncRNA, 10 miRNA and 22 mRNA, and we constructed the ceRNA network. Furthermore, we analyzed the functions and pathways of 22 differentially expressed mRNA. Then, univariate and multivariate Cox regression analyses of these 89 lncRNA and overall survival were explored. Nine lncRNA were finally screened out in the training group. The patients were divided into high‐risk and low‐risk groups according to the 9 lncRNA and low‐risk scores having better clinical overall survival (P < .01). Furthermore, the receiver operating characteristic curve demonstrates the predicted role of the 9 lncRNA. The 9‐lncRNA signature was successfully proved in the testing group and the entire group. Finally, multivariate Cox regression analysis and stratification analysis further proved that the 9‐lncRNA signature was an independent factor to predict survival. In summary, the present study provides a deeper understanding of the lncRNA‐related ceRNA network in ccRCC and suggests that the 9‐lncRNA signature could serve as an independent biomarker to predict survival in ccRCC patients.

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“…[29][30][31][32] However, the specific molecular mechanism between the five lncRNAs and potential pathways remained unclear. Then, we identified the central genes relevant to BLCA disease.…”

Section: Discussionmentioning

confidence: 99%

“…Particularly speaking, the Jak-STAT pathway was also enriched in many lncRNA-mediated tumor cells, like colorectal, hepatocellular, endometrial carcinomas as well as breast cancer. [29][30][31][32] However, the specific molecular mechanism between the five lncRNAs and potential pathways remained unclear. Hence, to provide new clues and target spots for early detection and F I G U R E 7 A, The relationship between gene modules and clinical features, such as risk score on the basis of the 5-lncRNAs signature.…”

Section: Discussionmentioning

confidence: 99%

Identification of five long noncoding RNAs signature and risk score for prognosis of bladder urothelial carcinoma

Zhang

et al. 2019

J of Cellular Biochemistry

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Nowadays, an increasing number of studies illustrated that bladder urothelial cancer (BLCA) may act as the most common subtype of urological malignancies with a high rate of recurrence and metastasis. In this study, we attempted to establish a prognostic model and identify the possible pathway crosstalk. Long noncoding RNAs (lncRNAs) and mRNA expression and corresponding clinical information of patients with BLCA were downloaded from The Cancer Genome Atlas (TCGA). The differentially expressed genes analysis, univariate Cox analysis, the least absolute shrinkage, and selection operator Cox (LASSO Cox) regression model were then applied to identify five crucial lncRNAs (AC092725.1, AC104071.1, AL023584.1, AL132642.1, and AL137804.1). The multivariate cox analysis was utilized to calculate the regression coefficients (βi). The risk‐score model was subsequently constructed as follows: (0.13541AC092725.1) + (0.20968AC104071.1) + (0.1525AL023584.1) − (0.14768AL132642.1) + (0.14387AL137804.1). Nomogram and assessment of overall survival (OS) prediction were verificated by the receiver operating characteristic curve in the testing group. As to 3‐, 5‐year OS prediction, the area under curve (AUC) for the nomogram of training data set was 0.83 and 0.86. Besides, the AUC (0.883 and 0.879) presented excellent predictive power in the testing group. In addition, the calibration plots validated the predictive performance of the nomogram. Weighted correlation network analysis (WGCNA) coupled with functional enrichment analysis contributed to explore the potential pathways, including PI3K‐Akt, HIF‐1, and Jak‐STAT signaling pathways. Construction of the risk‐score model and data analysis were both derived from multiple packages on the basis of the R platform chiefly.

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LncRNA AB073614 induces epithelial- mesenchymal transition of colorectal cancer cells via regulating the JAK/STAT3 pathway

Abstract: All these results indicate that lncRNA AB073614 can induce the expression of EMT cell markers and regulate the process of EMT of CRC cells through regulating the JAK/STAT3 pathway activation.

Cited by 38 publications

References 29 publications

LINC00346 promotes hepatocellular carcinoma progression via activating the JAK‐STAT3 signaling pathway

LINC00346 promotes hepatocellular carcinoma progression via activating the JAK‐STAT3 signaling pathway

Integrated analysis of long noncoding RNA associated‐competing endogenous RNA as prognostic biomarkers in clear cell renal carcinoma

Identification of five long noncoding RNAs signature and risk score for prognosis of bladder urothelial carcinoma

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