LncRNA AC012360.1 facilitates growth and metastasis by regulating the <scp>miR</scp>‐139‐5p/<scp>LPCAT1</scp> axis in hepatocellular carcinoma

Zhao, Yun; Liu, Ying; Shi, Xue

doi:10.1002/tox.23856

Cited by 4 publications

(2 citation statements)

References 47 publications

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“…A CCK-8 assay was conducted to assess HCC cell viability s (Zhao et al 2023). The transfected cells (1.5 × 10 3 cells/well) were grown in 96-well plates for 48 h. Next, CCK-8 reagent (10 µL) was added for incubation for 2 h. The viability was determined by detecting the absorbance at 450 nm using a microplate reader (Bio-Rad, Hercules, CA, USA).…”

Section: Cell Counting Kit-8 Assaymentioning

confidence: 99%

LINC00942 inhibits ferroptosis and induces the immunosuppression of regulatory T cells by recruiting IGF2BP3/SLC7A11 in hepatocellular carcinoma

Jin,

Hui,

Liu

et al. 2024

Funct Integr Genomics

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Hepatocellular carcinoma (HCC) is a common malignant tumor with a high recurrence rate and a poor prognosis. Long intergenic nonprotein coding RNA 942 (LINC00942) is reported to be related to ferroptosis and the immune response in HCC and serves as an oncogene in various cancers. This research aimed to explore the contribution of LINC00942 in HCC progression. Functional assays were used to evaluate the functional role of LINC00942 in vitro and in vivo. Mechanistic assays were conducted to assess the association of LINC00942 with insulin-like growth factor 2 mRNA binding protein 3 (IGF2BP3) and solute carrier family 7 member 11 (SLC7A11) and the regulatory pattern of LINC00942 in HCC cells. LINC00942 was found to exhibit upregulation in HCC tissue and cells. LINC00942 facilitated HCC cell proliferation, suppressed ferroptosis, and converted naive CD4+ T cells to inducible Treg (iTreg) cells by regulating SLC7A11. Furthermore, SLC7A11 expression was positively modulated by LINC00942 in HCC cells. IGF2BP3 was a shared RNA-binding protein (RBP) for LINC00942 and SLC7A11. The binding between the SLC7A11 3′ untranslated region and IGF2BP3 was verified, and LINC00942 was found to recruit IGF2BP3 to promote SLC7A11 mRNA stability in an m6A-dependent manner. Moreover, mouse tumor growth and proliferation were inhibited, and the number of FOXP3+CD25+ T cells was increased, while ferroptosis was enhanced after LINC00942 knockdown in vivo. LINC00942 suppresses ferroptosis and induces Treg immunosuppression in HCC by recruiting IGF2BP3 to enhance SLC7A11 mRNA stability, which may provide novel therapeutic targets for HCC.

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Section: Cell Counting Kit-8 Assaymentioning

confidence: 99%

LINC00942 inhibits ferroptosis and induces the immunosuppression of regulatory T cells by recruiting IGF2BP3/SLC7A11 in hepatocellular carcinoma

Jin,

Hui,

Liu

et al. 2024

Funct Integr Genomics

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“…The m6A-associated lncRNAs are identi ed as predict factors for the immune in ltration and prognosis of thyroid cancer [10] . In HCC, lncRNA AC012360.1 facilitates growth and metastasis by regulating the miR-139-5p/LPCAT1 axis [11] , and the conserved LncRNA DIO3OS restricts HCC stemness by interfering with NONO-mediated nuclear export of ZEB1 mRNA [12] . In recent years, lncRNAs have been reported to mediate various cell death modes to participate in the occurrence and progression of HCC.…”

Section: Introductionmentioning

confidence: 99%

Disulfidptosis-related lncRNAs are potential biomarkers for predicting prognoses in patients with hepatocellular carcinoma

Ma,

Liu,

et al. 2023

Preprint

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Objective: Hepatocellular carcinoma (HCC) is the most common type of liver cancer. Disulfidptosis is a newly discovered mechanism of programmed cell death. However, the role of disulfidptosis - related lncRNAs (DRlncRNAs) in HCC remains unclear. The purpose of this study is to establish the prognostic model of DRlncRNAs and explore its prognostic value in HCC. Materials and methods: The relevant clinical data and RNA-seq were obtained from the Cancer Genome Atlas (TCGA) database. The DRlncRNAs were identified via univariate and multivariate Cox regression, lasso algorithm analysis, and then established the prognostic model. Kaplan-Meier survival analysis, receiver operating characteristic (ROC) curves, principal component analysis (PCA), univariate and multivariate Cox regression analysis, functional enrichment annotation and the nomogram were used to assess the reliability of risk model. Furthermore, the potential immunotherapeutic signatures and drug sensitivity prediction were also performed. The RT-qPCR was applied to identify the expression of DRlncRNAs. Results:We constructed a prognostic model with 7 DRlncRNAs and proved the model could well predict the survival and prognosis of HCC patients. Immune correlation analysis suggested that low-risk patients had better immunotherapeutic outcomes. Drug prediction showed that Erlotinib, Gefitinib, Savolitinib, Osimertinib, Lapatinib, Afatinib and Crizotinib were more effective in low-risk patients; Sorafenib, Selumetinib, and Axitinib were more effective in high-risk patients. Finally, the expression of DRlncRNAs in normal liver and HCC cell lines were testified by RT-qPCR. Discussion and Conclusions: We constructed a risk model and provided a new direction for diagnosing and treating HCC.

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Modulation of Long Non-coding RNAs in Cancer

da Silva,

da Cunha Agostini,

Almeida

2023

Handbook of Cancer and Immunology

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LncRNA AC012360.1 facilitates growth and metastasis by regulating the miR‐139‐5p/LPCAT1 axis in hepatocellular carcinoma

Cited by 4 publications

References 47 publications

LINC00942 inhibits ferroptosis and induces the immunosuppression of regulatory T cells by recruiting IGF2BP3/SLC7A11 in hepatocellular carcinoma

LINC00942 inhibits ferroptosis and induces the immunosuppression of regulatory T cells by recruiting IGF2BP3/SLC7A11 in hepatocellular carcinoma

Disulfidptosis-related lncRNAs are potential biomarkers for predicting prognoses in patients with hepatocellular carcinoma

Modulation of Long Non-coding RNAs in Cancer

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