LncRNA APOA1-AS facilitates proliferation and migration and represses apoptosis of VSMCs through TAF15-mediated SMAD3 mRNA stabilization

Wang, Jixiang; Cai, Ying; Hui, Lisa; Zhang, Fugeng; Zheng, Jingwei

doi:10.1080/15384101.2021.1951940

Cited by 19 publications

(18 citation statements)

References 37 publications

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“…VSMCs are the major cell type in the artery. VSMCs migration, proliferation, and inflammatory factor secretion are involved in the important pathological processes of several vascular occlusive diseases, including CAS, and are involved in all stages of CAS [ 16 , 17 ]. The migration and proliferation of VSMCs are regulated by cytokines, growth factors, and other stimuli.…”

Section: Discussionmentioning

confidence: 99%

IL-1β promotes A7r5 and HASMC migration and invasion via the p38-MAPK/Angpt-2 pathway

Pei

Yang

et al. 2022

Eur J Med Res

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The migration, proliferation, and inflammatory factor secretion of vascular smooth muscle cells (VSMCs) are involved in the important pathological processes of several vascular occlusive diseases, including coronary atherosclerosis (CAS). Interleukin 1β(IL-1β), as a bioactive mediator of VSMC synthesis and secretion, can promote the pathological progress of CAS. In this study, we further explored the underlying molecular mechanisms by which IL-1β regulates VSMC migration, invasion. We pretreated A7r5 and HASMC with IL-1β for 24 h, and measured the expression of IL-1β, proliferating cell nuclear antigen (PCNA), cyclin D1, matrix metalloproteinase 2 (MMP2) and matrix metalloproteinase 2 (MMP9) in the cells by Western blotting. Cell migration and invasion ability were measured by Transwell and wound healing assays. Cell viability was measured by an MTT assay. We found that IL-1β upregulated the expression of proliferation-related proteins (PCNA and Cyclin D1) in A7r5 and HASMC, and induces the secretion of MMP2 and MMP9, promotes cell invasion and migration. In addition, in A7r5 and HASMCs treated with IL-1β, the expression of Angiopoietin-2 (Angpt-2) increased in a time-dependent manner, transfection with si-Angpt-2 suppressed cell migration and invasion, with downregulated MMP2 and MMP9 expression. Parallelly, we further found that the p38-MAPK pathway is activated in cells induced by IL-1β, p38-MAPK inhibitors can down-regulate the expression of Angpt-2. Collectively, these data demonstrated that IL-1β promotes A7r5 and HASMC migration and invasion via the p38-MAPK/Angpt-2 pathway.

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Section: Discussionmentioning

confidence: 99%

IL-1β promotes A7r5 and HASMC migration and invasion via the p38-MAPK/Angpt-2 pathway

Pei

Yang

et al. 2022

Eur J Med Res

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“…VSMCs are the major cell type in the artery, VSMCs migration, proliferation, and in ammatory factor secretion are involved in the important pathological processes of several vascular occlusive diseases, including CAS, and are involved in all stages of CAS [16,17] . The migration and proliferation of VSMCs are regulated by cytokines, growth factors, and other stimuli.…”

Section: Discussionmentioning

confidence: 99%

IL-1β Promotes A7r5 and HASMC Migration and Invasion via the p38-MAPK/Ang2 Pathway

Pei

Yang

et al. 2022

Preprint

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Background: The migration, proliferation, and inflammatory factor secretion of vascular smooth muscle cells (VSMCs) are involved in the important pathological processes of several vascular occlusive diseases, including coronary atherosclerosis (CAS). IL-1β, as a bioactive mediator of VSMC synthesis and secretion, can promote the pathological progress of CAS. In this study, we further explored the underlying molecular mechanisms by which IL-1β regulates VSMC migration, invasion.Methods: We pretreated A7r5 and HASMC with IL-1β for 24 hours, and measured the expression of IL-1β, PCNA, cyclin D1, MMP2 and MMP9 in the cells by Western blotting. Cell migration and invasion ability were measured by Transwell and wound healing assays. Cell viability was measured by an MTT assay. Results: We found that IL-1β up-regulated the expression of proliferation-related proteins (PCNA and Cyclin D1) in A7r5 and HASMC, and induces the secretion of MMP2 and MMP9, promotes cell invasion and migration. In addition, in A7r5 and HASMCs treated with IL-1β, the expression of Ang2 increased in a time-dependent manner, transfection with si-Ang2 suppressed cell migration and invasion, with down-regulated MMP2 and MMP9 expression. In parallely, we further found that the p38-MAPK pathway is activated in cells induced by IL-1β, p38-MAPK inhibitors can down-regulate the expression of Ang2. Conclusions: These data demonstrated that IL-1β promotes A7r5 and HASMC migration and invasion via the p38-MAPK/Ang2 pathway.

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“…TAF15 (TATA-box-binding protein-associated Factor 15) is a kind of RNA or DNA binding protein that belongs to the FUS-EWS-TAF15 (FET) family of proteins, which are involved in RNA splicing, transcription, mRNA transport, signalling, modification, translation and maintenance of genome integrity 6 – 9 . FET proteins are mainly present in the cell nucleus 10 , however, they are also present in the cell surface and cytoplasm 11 , 12 .…”

Section: Introductionmentioning

confidence: 99%

“…FET proteins are mainly present in the cell nucleus 10 , however, they are also present in the cell surface and cytoplasm 11 , 12 . Therefore, they have an extended function, including regulation and interaction with a number of various proteins 9 , 13 – 15 . FUS (fused in sarcoma/translocated in liposarcoma, FUS/TLS), a member of the FET family, can interact with lncRNA-MIR137HG and ultimately promote GC progression 16 .…”

Section: Introductionmentioning

confidence: 99%

“…Accumulating studies have shown that TAF15 is involved in the processes of human cancers, including extraskeletal myxoid sarcomas, leukaemia, human neuroblastoma, and lung squamous cell carcinoma 18 – 21 . In addition, LncRNAs or circRNAs can combine with TAF15 to stabilize downstream target genes such as SMAD3 mRNA, SOCS3 mRNA, and HMGB3 mRNA to facilitate cell proliferation and migration 9 , 15 , 22 . Moreover, lncRNA GAS5 binding to TAF15 could increase HIF1A expression, resulting in promotion of wound healing in diabetic foot ulcers 23 .…”

Section: Introductionmentioning

confidence: 99%

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TAF15 promotes cell proliferation, migration and invasion of gastric cancer via activation of the RAF1/MEK/ERK signalling pathway

Guo

et al. 2023

Sci Rep

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TATA-box-binding protein-associated Factor 15 (TAF15), a member of the FUS/EWS/TAF15 (FET) family, contributes to the progression of various tumours. However, the role and molecular mechanism of TAF15 in gastric cancer (GC) progression are still unknown. In this study, we found that TAF15 was significantly upregulated in GC tumour tissues and cell lines. Overexpression of TAF15 was associated with a larger tumour size, high pathologic stage and high T stage of GC. TAF15 knockdown suppressed the proliferation, migration and invasion of GC cells in vitro and inhibited the tumour growth in vivo. Additionally, TAF15 knockdown led to the significant reductions in the phosphorylation levels of RAF1, MEK and ERK1/2, while total RAF1, MEK and ERK1/2 exhibited no significant change in GC cell lines. In summary, TAF15 is overexpressed in GC tumour tissues and cell lines, and promotes cell proliferation, migration and invasion in GC via the RAF1/MEK/ERK signaling pathway, which suggests that TAF15 might be a potential molecular diagnostic marker or therapeutic target for GC.

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LncRNA APOA1-AS facilitates proliferation and migration and represses apoptosis of VSMCs through TAF15-mediated SMAD3 mRNA stabilization

Cited by 19 publications

References 37 publications

IL-1β promotes A7r5 and HASMC migration and invasion via the p38-MAPK/Angpt-2 pathway

IL-1β promotes A7r5 and HASMC migration and invasion via the p38-MAPK/Angpt-2 pathway

IL-1β Promotes A7r5 and HASMC Migration and Invasion via the p38-MAPK/Ang2 Pathway

TAF15 promotes cell proliferation, migration and invasion of gastric cancer via activation of the RAF1/MEK/ERK signalling pathway

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