LncRNA B4GALT1-AS1 promotes non-small cell lung cancer cell growth via increasing ZEB1 level by sponging miR-144-3p

Abstract

Background: Long noncoding RNAs (lncRNAs) are emerging as key players in the development and progression of cancer. Several malignancies involve dysregulated long noncoding ribonucleic acids (lncRNAs) in non-small cell lung cancer cell growth and their aggressive phenotypes. LncRNA B4GALT1-AS1 is important in the advancement of various malignancies, although its contribution to non-small cell lung cancer (NSCLC) remains unexplored.Methods: LncRNA B4GALT1-AS1 in NSCLC tissues was detected and further validated in a cohort of non-small cell lung cancer tissues. The effects of lncRNA B4GALT1-AS1 on proliferation were determined by in vitro experiments. The B4GALT1-AS1-miR-144-3p-ZEB1 axis was assessed by dual-luciferase reporter and RNA immunoprecipitation (RIP) assays. Furthermore, the mechanism of B4GALT1-AS1 was investigated using loss-of-function assays in vitro. Results:We showed significant upregulation of B4GALT1-AS1 in cell lines and tissues of NSCLC. B4GALT1-AS1 knockdown impeded the in vitro proliferation-related characteristics of the NSCLC cells. The demonstration of the binding capacity of B4GALT1-AS1 and miR-144-3p was predicted by bioinformatics and luciferase reporter activity assay. The B4GALT1-AS1 and miR-144-3p interaction was shown by using rescue experiments. NSCLC has a positive association with its target, zinc finger e-box binding homeobox 1 (ZEB1).Conclusions: In summary, the progression of NSCLC was facilitated by lncRNA B4GALT1-AS1 via interaction with miR-144-3p and positive regulation of ZEB1 expression.

LncRNA HOXD-AS2 regulates miR-3681-5p/DCP1A axis to promote the progression of non-small cell lung cancer

“…LncRNAs, with a length over 200 nt and no protein-coding potential, are differentially expressed and exert pivotal roles in modulating cellular activities in diverse carcinomas (10)(11)(12). For examples, lncRNA OXCT1-AS1 could boost proliferation of NSCLC cells through targeting miR-195/CCNE1 axis (13); lncRNA B4GALT1-AS1 accelerates NSCLC growth via elevating ZEB1 level via sponging miR-144-3p (14). Among these lncRNAs, HOXD cluster antisense RNA 2 (HOXD-AS2) is a demonstrated oncogenic lncRNA in human carcinomas.…”

Downregulation of long noncoding RNA B4GALT1-AS1 is associated with breast cancer development

“…The evidence showed that B4GALT1-AS1 by increasing the isolation of miR-30e resulted in increased expression of transcription factor (SOX9) 16 . Furthermore, the interaction between B4GALT1-AS1 and miR-144-3p regulated ZEB1 expression and increased the progression and tumorigenesis of the NSCLC cell line 31 . Further studies highlighted that the function of B4GALT1-AS1 was associated, at least partly, with two key proteins, HuR and YAP, which have been identified to provide roles in the development of cancers 32 .…”

Downregulation of long noncoding RNA B4GALT1-AS1 is associated with Breast Cancer development

“…The evidence showed that B4GALT1-AS1 by increasing the isolation of miR-30e resulted in increased expression of transcription factor (SOX9) [16]. Furthermore, the interaction between B4GALT1-AS1 and miR-144-3p regulated ZEB1 expression and increased the progression and tumorigenesis of the NSCLC cell line [29]. Further studies highlighted that the function of B4GALT1-AS1 was associated, at least partly, with two key proteins, HuR and YAP, which have been identi ed to provide roles in the development of cancers [30].…”