LncRNA cancer susceptibility 20 regulates the metastasis of human gastric cancer cells via the miR-143-5p/MEMO1 molecular axis

Abstract

BACKGROUND Gastric cancer (GC) is considered as one of the most widespread malignancies. Emerging evidence has shown that lncRNAs can function as important oncogenes or tumor suppressors during GC progression. AIM To investigate the effect and mechanism of lncRNA cancer susceptibility 20 (CASC20) in the proliferation and metastasis of GC cells. METHODS Data mining and clinical samples were used to evaluate the expression of CASC20 in GC and adjacent tissues. CASC20 was down-regulated in GC cells by short-interfering RNA. Cell proliferation was evaluated by CCK-8 assay, and cell migration and invasion were detected by wound healing and Transwell assays. The expressions of proteins related to epithelial-mesenchymal transition were detected by western blot assay. RESULTS The expression of CASC20 was increased in GC tumor tissues and various GC cell lines. High CASC20 expression was correlated with a high risk of lymphatic metastasis and poor prognosis in GC patients. In vitro assays showed that silencing CASC20 reduced cell proliferation, migration, and invasion in GC cells. Mechanistic studies revealed that CASC20 exhibits oncogenic functions by regulating MEMO1 expression through competitive endogenous binding to miR-143-5p, leading to induction of epithelial-mesenchymal transition. CONCLUSION Our findings indicate that CASC20 serves as a tumor promoter by regulating metastasis in GC via the miR-143-5p/MEMO1 axis. CASC20 may be a potential therapeutic target for GC.

Construction of a Cuproptosis‐Related lncRNA Prognostic Model for Bladder Urothelial Carcinoma and Screening of Potential Drugs

“…In this study, 11 lncRNAs with independent prognostic significance were screened using univariate Cox regression analysis, Lasso regression analysis, and multivariate Cox regression analysis to construct a prognostic model. Previous studies have shown that CASC20 is involved in the carcinogenesis of gastric cancer by competitively binding with Mir-143-5p and regulating the MEMO1 expression, thereby inducing epithelial-mesenchymal transition [15]. The SCAMP1 is a protein-coding gene.…”

Characterization of tumor immune microenvironment and cancer therapy for head and neck squamous cell carcinoma through identification of a genomic instability-related lncRNA prognostic signature

“…These data suggested that LINC01711 might play a tumor-promoting role in HNSCC development. lncRNA cancer susceptibility 20 (CASC20) was reported to serve as a tumor promoter by promoting the metastasis of human gastric cancer cells by the miR-143-5p/MEMO1 molecular axis ( Shan et al, 2022 ). LINC01843 increased expression in patients and correlated with poor survival in lung adenocarcinoma ( Li et al, 2020 ; Zheng et al, 2021 ) and colon cancer ( Zhou et al, 2018 ).…”