LncRNA CASC9 attenuates lactate dehydrogenase-mediated oxidative stress and inflammation in spinal cord injury via sponging miR-383-5p

Guan, Congjin; Wang, Yi

doi:10.1007/s10753-020-01387-7

Cited by 27 publications

(12 citation statements)

References 28 publications

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“…Notably, neuroglial and vascular endothelial cells are stimulated in SCI, releasing inflammatory cytokines and chemokines to induce inflammation; PC12 cell is a neural cell line commonly used to establish in vitro LPS-induced neuronal cell inflammation model, in which the expression of inflammatory factors (IL-6, IL-1β, IL-8, and TNF-a) are upregulated, consistent with that in SCI animal models [24]. Although whether the two are completely consistent remains uncertain, LPS-treated PC12 cells could be used to mimic the inflammatory response in SCI animals and has thus been used in a number of studies on SCI [25][26][27].…”

Section: Discussionmentioning

confidence: 86%

MicroRNA‐145‐Mediated KDM6A Downregulation Enhances Neural Repair after Spinal Cord Injury via the NOTCH2/Abcb1a Axis

Gao

Yin

et al. 2021

Oxidative Medicine and Cellular Longevity

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Spinal cord injury (SCI) causes a significant physical, emotional, social, and economic burden to millions of people. MicroRNAs are known players in the regulatory circuitry of the neural repair in SCI. However, most microRNAs remain uncharacterized. Here, we demonstrate the neuroprotection of microRNA-145 (miR-145) after SCI in vivo and in vitro. In silico analysis predicted the target gene KDM6A of miR-145. The rat SCI model was developed by weight drop, and lipopolysaccharide- (LPS-) induced PC12 cell inflammatory injury model was also established. We manipulated the expression of miR-145 and/or KDM6A both in vivo and in vitro to explain their roles in rat neurological functional recovery as well as PC12 cell activities and inflammation. Furthermore, we delineated the mechanistic involvement of NOTCH2 and Abcb1a in the neuroprotection of miR-145. According to the results, miR-145 was poorly expressed and KDM6A was highly expressed in the spinal cord tissue of the SCI rat model and LPS-induced PC12 cells. Overexpression of miR-145 protects PC12 cells from LPS-induced cell damage and expedites neurological functional recovery of SCI in rats. miR-145 was validated to target and downregulate the demethylase KDM6A expression, thus abrogating the expression of Abcb1a by promoting the methylation of NOTCH2. Additionally, in vivo findings verified that miR-145 expedites neuroprotection after SCI by regulating the KDM6A/NOTCH2/Abcb1a axis. Taken together, miR-145 confers neuroprotective effects and enhances neural repair after SCI through the KDM6A-mediated NOTCH2/Abcb1a axis.

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Section: Discussionmentioning

confidence: 86%

MicroRNA‐145‐Mediated KDM6A Downregulation Enhances Neural Repair after Spinal Cord Injury via the NOTCH2/Abcb1a Axis

Gao

Yin

et al. 2021

Oxidative Medicine and Cellular Longevity

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“…Furthermore, the DEmiRs were not the same as that observed for young males, with no common up or downregulated miRs on day 1 and only two miRNAs (miR-383-5p, miR-1249-3p) were downregulated on day 4 in both young females and young males. Downregulation of miR-383-5p may provide a neuroprotection against inflammation and associated oxidative stress [61]. Thus, for young animals, the initial response on day 1 is mainly a downregulation of miRs; although the specific miRs differ across sex, suggesting a sexually dimorphic response to sepsis.…”

Section: Young Adult Female Micementioning

confidence: 99%

“…Very few miRs were similarly modified across age or sex, particularly for day 1. For some miRs that were consistently downregulated in young males on day 1 and day 4 (miR-let-7d-3p) or downregulated in young males and females on day 4 (miR-383-5p), previous research suggest that downregulation is neuroprotective against inflammation and associated oxidative stress [41,42,61]. Similarly, for miRs that exhibited an increase on day 4, in young and old septic males (miR-223-3p, miR-544-5p, miR-219a-5p, miR-15a-5p), the increased expression has been linked to inhibition of inflammation and neuroprotection [47][48][49][50][51][52][53][54].…”

Section: Figure 3 Direction Of Mrna Expression Associated With Increased Mir Expression On Day 1 and Day 4 Post-sepsismentioning

confidence: 99%

Influence of age and sex on microRNA response and recovery in the hippocampus following sepsis

Rani

Barter

Kumar

et al. 2022

Aging

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Sepsis, defined as a dysregulated host immune response to infection, is a common and dangerous clinical syndrome. The excessive host inflammatory response can induce immediate and persistent cognitive decline, which can be worse in older individuals. Sex-specific differences in the outcome of infectious diseases and sepsis appear to favor females. We employed a murine model to examine the influence of age and sex on the brain's microRNA (miR) response following sepsis. Young and old mice of both sexes underwent cecal ligation and puncture (CLP) with daily restraint stress. Expression of hippocampal miR was examined in age-and sex-matched controls at 1 and 4 days post-CLP. Few miR were modified in a similar manner across age or sex and these few miR were generally associated with neuroprotection against inflammation. Similar to previous work examining transcription, young females exhibited a better recovery of the miR profile from day 1 to day 4, relative to young males and old females. For young males and all female groups, the initial response mainly involved a decrease in miR expression. In contrast, old males exhibited only upregulated miR on day 1 and day 4 and many of the miR upregulated on day 1 and day 4 were linked to neurodegeneration, increased neuroinflammation, and cognitive impairment. The results emphasize age and sex differences in epigenetic mechanisms that likely contribute to susceptibility or resilience to cognitive impairment due to sepsis.

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“…Studies have shown that the secondary spinal cord injury may lead to the further neurodegenerative diseases (Austin & Fehlings, 2008;López-Serrano et al, 2016). It has been found that the oxidative stress and inflammation play an important role in spinal cord injury (Zhaohui & Shuihua, 2020;Guan & Wang, 2021). Therefore, how to reduce the oxidative stress and inflammation is the focus of clinical treatment of spinal cord injury.…”

Section: Introductionmentioning

confidence: 99%

Extraction of proanthocyanidins from grape seeds and their protective effect on spinal cord injury in rats

et al. 2022

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Proanthocyanidins were extracted and purified from grape seeds, and the protective effect and mechanism of proanthocyanidins on spinal cord injury were explored. After extraction optimization and purification, the proanthocyanidins with purity of 92.53% were obtained. The rats were divided into control, model and treatment groups. The spinal cord injury model was established in model and treatment groups. Then, the treatment group was treated with 40 mg/kg proanthocyanidins. On the 1st, 3rd and 7th day after modeling, compared with model group, in model group the Basso-Beattie-Bresnahan scores were significantly decreased (p < 0.05). On the 7th day after modeling, compared with model group, in treatment group the spinal cord tissue superoxide dismutaseand glutathione peroxidase levels were significantly increased (p < 0.05), the malondialdehyde level was significantly decreased (p < 0.05), the necrosis factor α and interleukin 1βlevels were significantly decreased (p < 0.05), and the interleukin 10 level was significantly increased (p < 0.05). In conclusion, proanthocyanidins have protective effect on spinal cord injury in rats. The mechanisms may be related to its reducing oxidative stress and inflammation in spinal cord tissue.

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LncRNA CASC9 attenuates lactate dehydrogenase-mediated oxidative stress and inflammation in spinal cord injury via sponging miR-383-5p

Cited by 27 publications

References 28 publications

MicroRNA‐145‐Mediated KDM6A Downregulation Enhances Neural Repair after Spinal Cord Injury via the NOTCH2/Abcb1a Axis

MicroRNA‐145‐Mediated KDM6A Downregulation Enhances Neural Repair after Spinal Cord Injury via the NOTCH2/Abcb1a Axis

Influence of age and sex on microRNA response and recovery in the hippocampus following sepsis

Extraction of proanthocyanidins from grape seeds and their protective effect on spinal cord injury in rats

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