LncRNA CCAT2 promoted osteosarcoma cell proliferation and invasion

Liu, Yan; Wu, Xiangkun; Yin, Xianzhe; Feng, Du; Liu, Yongxi; Ding, Xunmeng

doi:10.1111/jcmm.13518

Cited by 46 publications

(35 citation statements)

References 40 publications

(52 reference statements)

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“…In recent years, lncRNAs are reported to have important epigenetic regulatory roles in diverse biologic cellular processes. Studies have indicated that lncRNAs play important regulatory roles in tumorigenesis and metastasis [10,11]. Our study demonstrated that lncRNA HIF2PUT may play an anti-oncogene role in the development of osteosarcoma.…”

Section: Discussionmentioning

confidence: 52%

LncRNA HIF2PUT inhibited osteosarcoma stem cells proliferation, migration and invasion by regulating HIF2 expression

Ding

Wang

Chu

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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Purpose: The function of lncRNAs in cancer stem cells (CSCs) remains to be elucidated. The present study aimed to investigate the regulating role of a novel lncRNA, hypoxia-inducible factor-2a (HIF-2a) promoter upstream transcript (HIF2PUT), in osteosarcoma stem cells. Methods: The expression of lncRNA HIF2PUT and HIF-2a in osteosarcoma stem cell lines and tissues was monitored by real-time PCR and western blot. The proliferation ability was examined by MTT assay when HIF2PUT overexpression or knockdown. The self-renewing capabilities of the cells were assessed by spheroid formation assay. The migration and invasion of cells were monitored by wound-healing assay and transwell cell assay, respectively. The correlation of HIF2PUT and HIF-2a expression was determined in osteosarcoma cancer tissues. Results: LncRNA HIF2PUT and HIF-2a were downregulated in osteosarcoma cell lines. HIF2PUT exhibited a significant decline in proliferation capacity. Wound healing and transwell assays showed that lncRNA overexpression inhibited osteosarcoma stem cell migration and invasion. HIF2PUT inhibited sphere formation in osteosarcoma stem cells. Increased HIF2PUT expression inhibited the enrichment of CD133 in osteosarcoma stem cells. There was a strong positive correlation between relative HIF2PUT level and relative HIF-2a level in the 30 paired osteosarcoma cancer tissues. Conclusions: Overexpression of lncRNA HIF2PUT significantly attenuated the proliferation, migration and invasion of osteosarcoma stem cells. Furthermore, we demonstrated that lncRNA overexpression inhibited the sphere-formation of osteosarcoma stem cells by downregulating HIF-2a. These findings suggest that lncRNA HIF2PUT may act as a tumour suppressor in osteosarcoma. LncRNA HIF2PUT/HIF-2a may be a novel therapeutic target in the treatment of osteosarcoma.

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Section: Discussionmentioning

confidence: 52%

LncRNA HIF2PUT inhibited osteosarcoma stem cells proliferation, migration and invasion by regulating HIF2 expression

Ding

Wang

Chu

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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“…17,18 FoxM1 plays various critical roles in the regulation of cell behavior, including differentiation, proliferation, and apoptosis. 17,18 FoxM1 plays various critical roles in the regulation of cell behavior, including differentiation, proliferation, and apoptosis.…”

Section: Discussionmentioning

confidence: 99%

MiR‐216b inhibits osteosarcoma cell proliferation, migration, and invasion by targeting Forkhead Box M1

Wang

Guo

et al. 2018

J of Cellular Biochemistry

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Osteosarcoma (OS) is considered the most common type of primary malignant bone tumor, which has a high rate of mortality in children and adolescents. However, the current treatment methods for OS are ineffective. Therefore, there is an urgent requirement to identify the critical targets. This study aimed to identify the roles and significance of microRNA‐216b (miR‐216b) in OS. To explore the cellular and molecular functions of miR‐216b and Forkhead Box M1 (FoxM1) in OS, the expression of miR‐216b and FoxM1 at the transcriptional level was measured using quantitative real‐time PCR (qRT‐PCR). Wound healing assay, 3‐[4,5‐dimethylthiazol‐2‐yl]‐2,5 diphenyltetrazolium bromide assay (MTT) assay, flow cytometry, and transwell invasion assay were conducted to study the function of miR‐216b and FoxM1 in OS cells. Dual luciferase reporter assay was performed to identify the relationships between miR‐216b and FoxM1. qRT‐PCR results revealed that miR‐216b expression was significantly downregulated, and FoxM1 was observed to be significantly upregulated in human OS cell lines (MG‐63) and tissues. MTT data showed that upregulation of miR‐216b expression led to cell growth inhibition in MG‐63 cells. The results of the invasion assay and wound healing assay illustrated that miR‐216b upregulation or FoxM1 downregulation could inhibit the invasion and migration in MG‐63 cells. In vivo, the tumor volume was significantly decreased by miR‐194 mimic treatment compared with the control group. Furthermore, the results of the luciferase assay indicated that FoxM1 is a direct target of miR‐216b. These findings may provide novel insights into the molecular mechanism of miR‐216b and FoxM1 in the progression of OS, and suggested that miR‐216b may serve as a potential tumor inhibitor of OS by targeting FoxM1.

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“…Among them, long non-coding RNAs (lncRNAs), which exceed 200 nucleotides in length and do not encode proteins [18][19][20], participate in multiple biological processes, including cell proliferation and invasion [21,22]. They are key regulators of cancer progression and modulate the transcription of cancer-related genes through chromatin modification and transcriptional processes [23][24][25].…”

Section: Introductionmentioning

confidence: 99%

Transcriptome analysis reveals the link between lncRNA-mRNA co-expression network and tumor immune microenvironment and overall survival in head and neck squamous cell carcinoma

et al. 2020

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Background: As the sixth most common cancer worldwide, head and neck squamous cell carcinoma (HNSCC) develops visceral metastases during the advanced stage of the disease and exhibits a low five-year survival rate. The importance of tumor microenvironment (TME) in tumor initiation and metastasis is widely recognized. In addition, accumulating evidence indicates that long non-coding RNA (lncRNA) is involved in crosstalk between TME and tumor cells. However, the lncRNA-associated regulators modulating the HNSCC microenvironment and progression remain largely unknown. Methods: The publicly available transcriptome data and matched clinical HNSCC data were collected from The Cancer Genome Atlas (TCGA). Immune scores (ISs) and stromal scores (SSs) of HNSCC TME were calculated using ESTIMATE algorithm. Weighted gene co-expression network analysis (WGCNA) was conducted to determine the coexpressed lncRNAs and protein-coding mRNAs.

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LncRNA CCAT2 promoted osteosarcoma cell proliferation and invasion

Cited by 46 publications

References 40 publications

LncRNA HIF2PUT inhibited osteosarcoma stem cells proliferation, migration and invasion by regulating HIF2 expression

LncRNA HIF2PUT inhibited osteosarcoma stem cells proliferation, migration and invasion by regulating HIF2 expression

MiR‐216b inhibits osteosarcoma cell proliferation, migration, and invasion by targeting Forkhead Box M1

Transcriptome analysis reveals the link between lncRNA-mRNA co-expression network and tumor immune microenvironment and overall survival in head and neck squamous cell carcinoma

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