LncRNA, CRNDE promotes osteosarcoma cell proliferation, invasion and migration by regulating Notch1 signaling and epithelial-mesenchymal transition

Li, Zheng; Tang, Yonghua; Xing, Wujun; Wang, Dong; Wang, Zhichou

doi:10.1016/j.yexmp.2017.12.002

Cited by 64 publications

(39 citation statements)

References 31 publications

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“…Total RNA was extracted using the TRIzol reagent, and cDNA was synthesized using SuperScript II Reverse Transcriptase (Invitrogen, Carlsbad, CA). Real‐time polymerase chain reaction (PCR) was then performed with the commercially available human EMT RT 2 Profiler PCR Array (Qiagen, Dusseldolf, Germany) according to the manufacturer's instructions . The data were normalized to glyceraldehyde 3‐phosphate dehydrogenase via the comparative C t method.…”

Section: Methodsmentioning

confidence: 99%

LncRNA NBR2 inhibits epithelial‐mesenchymal transition by regulating Notch1 signaling in osteosarcoma cells

Cai

et al. 2018

J of Cellular Biochemistry

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Long noncoding RNAs (lncRNAs) have been identified to have increasingly important roles in tumorigenesis, and they may serve as novel biomarkers for cancer therapy. Recent studies have demonstrated that lncRNA NBR2 (neighbor of BRCA1 gene 2), a novel identified lncRNA, is decreased in several cancers; however, the role of NBR2 in the development of osteosarcoma has not been elucidated. In our study, we found that NBR2 expression was downregulated in osteosarcoma tissues, and osteosarcoma cases with lower NBR2 expression exhibited a shorter overall survival time compared with those with higher NBR2 expression. NBR2 overexpression inhibited osteosarcoma cell proliferation, invasion, and migration but did not increase apoptosis. Furthermore, RNA-binding protein immunoprecipitation assays confirmed that NBR2 directly binds to Notch1 protein. Furthermore, overexpression of Notch1 in NBR2-overexpressing osteosarcoma cells reversed the effects of NBR2 on cell proliferation, invasion, migration, and epithelial-mesenchymal transition. The in vivo results showed that NBR2 overexpression inhibited tumor growth in nude mice that were inoculated with osteosarcoma cells. NBR2 overexpression also suppressed the messenger RNA (mRNA) expression of Notch1, N-cadherin, and vimentin and increased the mRNA expression of E-cadherin in the tumor tissues. These data indicated that NBR2 served as a tumor suppressor gene in osteosarcoma and inhibited osteosarcoma cell proliferation, invasion, and migration. The current study provides a novel insight and treatment strategy for osteosarcoma.

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Section: Methodsmentioning

confidence: 99%

LncRNA NBR2 inhibits epithelial‐mesenchymal transition by regulating Notch1 signaling in osteosarcoma cells

Cai

et al. 2018

J of Cellular Biochemistry

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“…14,15,27 Some previous studies have suggested that lncRNA CRNDE is associated with tumorigenesis and metastasis. 28,29 In the present study, serum levels of lncRNA CRNDE and lncRNA SNHG7 in patients underwent recurrence was significantly higher than that of patients with recurrencefree. Furthermore, serum levels of lncRNA CRNDE and lncRNA SNHG7 in patients who underwent death was also significantly higher than that of surviving patients.…”

Section: Discussionmentioning

confidence: 40%

<p>LncRNA CRNDE and lncRNA SNHG7 are Promising Biomarkers for Prognosis in Synchronous Colorectal Liver Metastasis Following Hepatectomy</p>

Zhang

Shi

Song

et al. 2020

CMAR

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Synchronous colorectal liver metastasis (SCLM) had limited availability of tools to predict survival and tumor recurrence. LncRNA CRNDE and lncRNA SNHG7 have been proven to be closely related to cancer progression. However, the predictive value of lncRNA CRNDE and lncRNA SNHG7 in cancer prognosis is still unclear. The purpose of this study was to investigate whether lncRNA CRNDE and lncRNA SNHG7 could be used as promising biomarkers for prognosis prediction of SCLM patients who underwent hepatectomy. Methods: The expression profile of lncRNA CRNDE and lncRNA SNHG7 in serum of SCLM patients was examined by qRT-PCR. The relationship between lncRNA expression and clinicopathological characteristics was analyzed. The Cox proportional-hazards regression model and Kaplan-Meier analysis were performed to analyze the association between lncRNA expression and overall survival (OS) and tumor recurrence of SCLM patients. Results: Levels of lncRNA CRNDE and lncRNA SNHG7 in patients who underwent recurrence or death were significantly higher than that of patients with recurrence-free or survival (P<0.01). Both lncRNA CRNDE high level and lncRNA SNHG7 high level showed a significant correlation with differentiation of primary tumor, invasion depth of primary focus, lymph node metastases, number of liver metastases, and liver metastasis grade. High levels of lncRNA CRNDE or lncRNA SNHG7 predicted shorter recurrence time, shorter OS time, higher recurrence rate and lower OS rate. Furthermore, lncRNA CRNDE and lncRNA SNHG7 were independent risk factors for high recurrence and poor OS in SCLM underwent hepatectomy. Conclusion: Taken together, lncRNA CRNDE and lncRNA SNHG7 could be promising biomarkers for prediction of OS and tumor recurrence in SCLM underwent hepatectomy.

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“…7 In renal cell carcinoma, Shao et al performed loss-of-function and gain-of-function assays and found that CRNDE enhanced the cell proliferation and growth both in vitro and in vivo through regulating Wnt/β-catenin signaling. 26,33 To investigate the molecular mechanism of CRNDE in regulating ccRCC cell motility, we found that CRNDE positively regulated the protein expression of Vimentin, ZEB1 and ZEB2, and negatively modulated E-cadherin protein expression. In our study, loss-offunction and gain-of-function approaches were performed to estimate the effect of CRNDE on ccRCC cell migration and invasion.…”

Section: Discussionmentioning

confidence: 99%

“…EMT has been suggested to be involved in CRNDEmediated tumor cell migration and invasion. 26,33 To investigate the molecular mechanism of CRNDE in regulating ccRCC cell motility, we found that CRNDE positively regulated the protein expression of Vimentin, ZEB1 and ZEB2, and negatively modulated E-cadherin protein expression. Thus, CRNDE regulates ccRCC cell migration and invasion through modulating EMT-associated genes.…”

Section: Discussionmentioning

confidence: 99%

LncRNA CRNDE is a biomarker for clinical progression and poor prognosis in clear cell renal cell carcinoma

Ding

Han

Xiang

et al. 2018

J of Cellular Biochemistry

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Colorectal neoplasia differentially expressed (CRNDE) served as an oncogenic long noncoding RNA (lncRNA) to be involved in the initialization and development of human cancers. However, the clinical significance and biological function of CRNDE in clear cell renal cell carcinoma (ccRCC) was not fully understood. In our study, we found CRNDE levels were increased in ccRCC tissue specimens and cell lines, and corrected with advanced clinical stage, large tumor size, lymph node metastasis, distant metastasis, and poor pathological grade in patients with ccRCC. Furthermore, levels of CRNDE were negatively correlated with overall survival of patients with ccRCC, and high-expression of CRNDE was an independent poor prognostic factor for patients with ccRCC. Moreover, loss-of-function and gain-of-function approaches showed CRNDE-enhanced ccRCC cell migration and invasion through modulating EMT-associated genes. In conclusion, CRNDE acts as an oncogenic lncRNA in ccRCC.

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LncRNA, CRNDE promotes osteosarcoma cell proliferation, invasion and migration by regulating Notch1 signaling and epithelial-mesenchymal transition

Cited by 64 publications

References 31 publications

LncRNA NBR2 inhibits epithelial‐mesenchymal transition by regulating Notch1 signaling in osteosarcoma cells

LncRNA NBR2 inhibits epithelial‐mesenchymal transition by regulating Notch1 signaling in osteosarcoma cells

<p>LncRNA CRNDE and lncRNA SNHG7 are Promising Biomarkers for Prognosis in Synchronous Colorectal Liver Metastasis Following Hepatectomy</p>

LncRNA CRNDE is a biomarker for clinical progression and poor prognosis in clear cell renal cell carcinoma

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