LncRNA CRNDE promotes the epithelial‐mesenchymal transition of hepatocellular carcinoma cells via enhancing the Wnt/β‐catenin signaling pathway

Abstract

Colorectal neoplasia differentially expressed (CRNDE) is a significantly upregulated long noncoding RNA in hepatocellular carcinoma (HCC). CRNDE could promote cell proliferation, migration, and invasion, while its molecular mechanisms were still largely unclear. In this study, we investigated the expression and function of CRNDE. CRNDE was significantly upregulated in tumor tissues compared with adjacent normal tissues. In vitro, we revealed that knockdown of CRNDE inhibited cell proliferation, migration, and cell invasion capacities in HCC. Animal studies indicated that CRNDE knockdown represses both growth and metastasis of HCC tumors in vivo. Moreover, knockdown of CRNDE suppressed the cell epithelial‐mesenchymal transition (EMT) process by increasing the expression of E‐cadherin and ZO‐1, whereas, decreasing the expression of N‐cadherin, slug, twist, and vimentin in HCC cells. We also revealed that knockdown of CRNDE suppressed the Wnt/β‐catenin signaling in HCC. Thus, CRNDE could modulate EMT of HCC cells and knockdown of CRNDE impaired the mesenchymal properties. CRNDE increased invasion of HCC cells might be through activating the Wnt/β‐catenin signaling pathway.

Long non‑coding RNA CCAT2 promotes prostate cancer cell proliferation and invasion by regulating the Wnt/β‑catenin signaling pathway

“…For example, lncRNA HOXA distal transcript antisense RNA regulates osteosarcoma by activating the Wnt/β-catenin signaling pathway ( 51 ). Furthermore, lncRNA small nucleolar RNA host gene 20 promotes glioblastoma and tumor progression by activating the PI3K/Akt/mTOR signaling pathway ( 52 ) and lncRNA colorectal neoplasia differentially expressed promotes liver cancer by enhancing the Wnt/β-catenin signaling pathway ( 53 ). Several studies have demonstrated that the typical Wnt/β-catenin signaling pathways are involved in a variety of cellular processes, including cell proliferation, autophagy, cell cycle, cell differentiation and tumorigenesis ( 54 – 57 ).…”

The competitive endogenous RNA regulatory network reveals potential prognostic biomarkers for overall survival in hepatocellular carcinoma

“…The lncRNA MCM3AP‐AS1 can upregulate the expression of FOXA1 by competitive combination with miR‐194‐5p and promotes HCC cell proliferation . The lncRNA CRNDE can promote the invasive ability of HCC cell through the Wnt/β‐catenin pathway . The high expression of PVT1 can promote the occurrence of HCC through effecting expression level of miR‐150 and HIG2 .…”

Knockdown of lncRNA CCEPR suppresses colorectal cancer progression

“…An increasing number of lncRNAs have been reported to serve as important mediators in human cancers (27,28). Specific lncRNAs are significantly deregulated in colorectal cancer and have been demonstrated to promote tumour growth and metastasis.…”