LncRNA DLEU1 contributes to colorectal cancer progression via activation of KPNA3

Liu, Tianyou; Han, Zhiyang; Li, Huanyu; Zhu, Yuekun; Sun, Ziquan; Zhu, Aiguo

doi:10.1186/s12943-018-0873-2

Cited by 121 publications

(107 citation statements)

References 42 publications

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“…14 For example, lncRNA DLEU1 promoted CRC cell progression through regulating KPNA3. 15 Moreover, lncRNA CRNDE induced cell proliferation and chemoresistance in CRC. 16 lncRNA BANCR has been determined to relate to CRC poor prognosis.…”

Section: Introductionmentioning

confidence: 99%

<p>HNF1A-AS1 Regulates Cell Migration, Invasion and Glycolysis via Modulating miR-124/MYO6 in Colorectal Cancer Cells</p>

Guo¹,

Zhang²,

Liu³

et al. 2020

OTT

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Background: Accumulating evidence determined that lncRNAs play multiple roles in cell progression in colorectal cancer (CRC). Long noncoding RNA (lncRNA) hepatocyte nuclear factor 1 homeobox A (HNF1A)-antisense RNA 1 (AS1) has been identified to affect cell growth and disease diagnosis in various cancers, including CRC. However, the underlying regulatory mechanism of HNF1A-AS1 in cell progression and glycolysis has not been fully explored in CRC. Materials and Methods: The expression of HNF1A-AS1, microRNA-124 (miR-124) and Myosins of class VI (MYO6) was detected using reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The analysis of glucose consumption, lactate production and hexokinase 2 (HK2) protein level was used to assess glycolysis in cells. The protein level of HK2 and MYO6 was measured with Western blot. Cell migration and invasion were evaluated using the transwell assay. The relationship among HNF1A-AS1, miR-124 and MYO6 was determined via luciferase reporter and RNA immunoprecipitation (RIP) assay. Results: In this study, we found that HNF1A-AS1 was upregulated in CRC tissues and cell lines. Functional experiments determined that reduction of HNF1A-AS1 or promotion of miR-124 inhibited cell migration and invasion as well as glycolysis in CRC cells. What' more, luciferase reporter assay manifested that miR-124 was a target of HNF1A-AS1 and MYO6 was a target mRNA of miR-124 in CRC cells. Additionally, reverse experiments showed that the effects of si-HNF1A-AS1 on colorectal cancer cells were impaired by anti-miR-124 and the effects of high miR-124 expression on CRC cells were rescued by upregulating MYO6. HNF1A-AS1 regulated MYO6 expression via targeting miR-124 in CRC cells. Conclusion: In this study, we first found that HNF1A-AS1 regulated cell migration, invasion and glycolysis via modulating miR-124/MYO6 in CRC cells.

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Section: Introductionmentioning

confidence: 99%

<p>HNF1A-AS1 Regulates Cell Migration, Invasion and Glycolysis via Modulating miR-124/MYO6 in Colorectal Cancer Cells</p>

Guo¹,

Zhang²,

Liu³

et al. 2020

OTT

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“…Increasingly, many lncRNAs have been reported to play important roles in multiply biological processes of osteosarcoma including tumour cell proliferation, invasion, migration, and chemoresistance [3,4]. LncRNA DLEU1 is overexpressed and exerts an oncogene function by regulating cell proliferation, migration and invasion in several types of cancers including bladder cancer [6], cervical cancer [7], oral squamous cell carcinoma [8], non-small cell lung cancer [9], and colorectal cancer [10]. However, the role of DLEU1 in osteosarcoma remains unclear.…”

Section: Introductionmentioning

confidence: 99%

Long noncoding RNA DLEU1 aggravates osteosarcoma carcinogenesis via regulating the miR-671-5p/DDX5 axis

Chen

Zhang

Wang

2019

Artificial Cells, Nanomedicine, and Biotechnology

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Long non-coding RNAs (LncRNAs) have been proven to be involved in the progression of osteosarcoma. LncRNA DLEU1 was found to act as an oncogene in various types of cancer. In this study, we aimed to explore the biological functions of DLEU1 in osteosarcoma and the specific mechanism. Our results showed that DLEU1 was highly expressed in osteosarcoma tissue specimens and cells. Downregulation of DLEU1 in osteosarcoma cells inhibited the cell proliferation, migration and invasion. Further mechanistic analysis and functional assays demonstrated that DLEU1 exerted its role by sponging microRNA (miR)-671-5p in osteosarcoma cells. Moreover, DEAD-box helicase 5 (DDX5) was confirmed as the target of miR-671-5p. Furthermore, rescue assays indicated that miR-671-5p inhibitor significantly reversed the effects of DLEU1 knockdown on osteosarcoma cell proliferation and invasion while restoration of DDX5 rescued the proliferation and invasion of osteosarcoma cells transfected with miR-671-5p mimics. In conclusion, DLEU1 acted as an oncogene in osteosarcoma by directly sponging miR-671-5p and regulating the expression of DDX5. These findings suggested that DLEU1 might be a novel therapeutic target in the treatment of osteosarcoma. ARTICLE HISTORY

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“…To further confirm the possible permeability of these peptide oligomer functions in gliomas, we chose DLEU1 for subsequent simulations. DLEU1 activates KPNA3, which is related to the increased proliferation and migration of cancer cells 53 . Some reports have indicated that DLEU1 can increase cell proliferation; our results showed that ORF1 and ORF8 in DLEU1 could encode small TM peptides.…”

Section: Discussionmentioning

confidence: 99%

Prediction of LncRNA Encoded Small Peptides in Glioma and The Oligomer Channel Functional Analysis Using in Silico Approaches

Cao

Lee

Zhang

et al. 2020

Preprint

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Glioma is lethal malignant brain cancers, many reports have shown that abnormalities in the behavior of water and ion channels play an important role in regulating glioma proliferation, migration, apoptosis and differentiation. Recently, new studies have suggested that some long noncoding RNAs (lncRNAs) containing small open reading frames (smORFs), can encode small peptides and form oligomers for water or ion regulation. However, because these peptides are difficult to identify, their functional mechanisms are far from being clearly understood. In this study, we used bioinformatic methods and softwares to identify and evaluate lncRNAs in gliomas that may encode small transmembrane peptides. Combining ab initio homology modeling, molecular dynamics simulations and energetic calculations, we constructed a predictive model and predicted the oligomer channel activity of peptides by identifying the lncRNA ORFs.We found that one key hub lncRNA, DLEU1, which contains two smORFs (ORF1 and ORF8) could encode small peptides that form pentameric channels. The mechanics of water and ion (Na + and Cl-) transport through this pentameric channel were simulated. The potential of mean force (PMF) of the H 2 O molecules along the two ORF-encoded peptide channels indicated that the energy barrier was different between ORF1 and ORF8. The ORF1-encoded peptide pentamer acted as a self-assembled water channel but not as an ion channel, and the ORF8 neither permeating ions nor water.This work provides new methods and theoretical support for further elucidation of the function of lncRNA-encoded small peptides and their role in cancer. Additionally, it provides a theoretical basis for drug development.

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LncRNA DLEU1 contributes to colorectal cancer progression via activation of KPNA3

Cited by 121 publications

References 42 publications

<p>HNF1A-AS1 Regulates Cell Migration, Invasion and Glycolysis via Modulating miR-124/MYO6 in Colorectal Cancer Cells</p>

<p>HNF1A-AS1 Regulates Cell Migration, Invasion and Glycolysis via Modulating miR-124/MYO6 in Colorectal Cancer Cells</p>

Long noncoding RNA DLEU1 aggravates osteosarcoma carcinogenesis via regulating the miR-671-5p/DDX5 axis

Prediction of LncRNA Encoded Small Peptides in Glioma and The Oligomer Channel Functional Analysis Using in Silico Approaches

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