LncRNA DNAJC3‐AS1 promotes the biological functions of papillary thyroid carcinoma via regulating the microRNA‐27a‐3p/CCBE1 axis

Abstract

Long noncoding RNA DNAJC3-AS1 (lncRNA DNAJC3-AS1) has been probed in many studies, while the regulatory mechanism of DNAJC3-AS1 on papillary thyroid carcinoma (PTC) via regulating microRNA (miR)-27a-3p remains inadequate. This research aims to depict the role of DNAJC3-AS1, miR-27a-3p, collagen, and calcium-binding EGF domain-containing protein 1 (CCBE1) on PTC development. DNAJC3-AS1, miR-27a-3p, and CCBE1 expression levels in PTC tissues and adjacent normal tissues were tested. The relation of DNAJC3-AS1, miR-27a-3p, and CCBE1 was analyzed. DNAJC3-AS1 and miR-27a-3p and CCBE1-related oligonucleotides were transfected into IHH-4 cells to investigate their role in PTC development. Cell tumorigenicity was detected by in vivo assay. DNAJC3-AS1 and CCBE1 expressed highly and miR-27a-3p expressed lowly in PTC. Downregulation of DNAJC3-AS1, upregulating miR-27a-3p or downregulating CCBE1 impaired the malignant behaviors of IHH-4 cells. Depletion of miR-27a-3p reversed the DNAJC3-AS1 suppression-induced phenotypic inhibition of IHH-4 cells. DNAJC3-AS1 bound to miR-27a-3p and CCBE1 as a target of miR-27a-3p. Our study highlights that DNAJC3-AS1 inhibits miR-27a-3p to promote CCBE1 expression, thereby facilitating PTC development. This study affords distinguished therapeutic strategies and novel research directions for PTC treatment.

Reveal the potential molecular mechanism of circRNA regulating immune-related mRNA through sponge miRNA in the occurrence and immune regulation of papillary thyroid cancer

“…CircRNA cRAPGEF5 is involved in inhibiting the growth and metastasis of renal cell carcinoma through sponge hsa-miR-27a-3p [ 29 ]. Hsa-miR-27a-3p has been verified to be involved in the development of PTC [ 30 ]. LINC01089 can block the malignant progression of TC by binding hsa-miR-27b-3p to increase the level of FBLN5 protein [ 31 ].…”

The diagnostic performance of microRNAs expression levels in cellular aspirate of solitary thyroid nodule overrides that of cytology

“…4 The role of multiple microRNAs in pathogenesis of TC was recently declared; lower expression levels of miR-30b was found to promote TC via induction of high expression levels of ubiquitin-specific protease-22 in thyroid cells and downregulation of miR-27a could facilitate the development of PTC through promoting the expression of collagen, and calcium-binding EGF domain-containing protein-1 in thyroid tissue. 5,6 On contrary, upregulated Mir-449a suppresses PTC development through binding to the 3' un-translated region of Metadherin and downregulating its expression. 7 The widespread use of diagnostic imaging and more sensitive diagnostic tools resulted in great increase in incidence of small and localized thyroid tumors; however, this may result in over-diagnosis of STN, and needs to be refined to guard against faulty surgicaldecision making.…”