LncRNA‐ENST00000501520 promotes the proliferation of malignant‐transformed BEAS‐2B cells induced with coal tar pitch mediated by target genes

Abstract

As a human carcinogen, coal tar pitch (CTP) can significantly increase the risk of lung cancer. However, the mechanism underlying CTP‐induced lung carcinogenesis has not been well understood. This study aims to explore the role of the LncRNA‐ENST00000501520 in the proliferation of malignant‐transformed human bronchial epithelial cells (BAES‐2B) induced by CTP extract for the first time. BEAS‐2B cells were stimulated with 2.4 μg/mL CTP extract, and then passaged for three times, which were named passage 1 and then passaged until passage 30 (named as CTP group). The ENST000001520 of cells in CTP group was interfered using siRNA. The results showed that ENST000001520 located in cell nucleus (>80%) had no or weak ability of protein encoding. After interference of ENST000001520, the migration and proliferation of cells in CTP group were inhibited, and the cell cycle was arrested in the G0/G1 phase; however, the apoptosis of cells in CTP group was promoted. The target genes (SKB1, CLTB, TAP2, PIPK2, and SOCS3) of ENST000001520 were screened out, and the mRNA and protein expression of SBK1 and SOCS3 was significantly decreased after ENST000001520 interference. SBK1 and SOCS3 may play a promoting role in occurrence and development of cancers. The study suggests that LncRNA‐ENST00000501520 could promote the proliferation in malignant‐transformed BEAS‐2B cells induced with CTP extract which may be mediated by target genes. This study may provide a new target for prevention and treatment of lung cancer.

SH3 domain‐binding kinase 1 promotes proliferation and inhibits apoptosis of cervical cancer via activating the Wnt/β‐catenin and Raf/ERK1/2 signaling pathways

“…Consistent with our results, the expression of SBK1 was facilitated by LncRNA ELFN1‐AS1 to promote proliferation and invasion in retinoblastoma 15 . SBK1 was also activated in the malignant transformation of pulmonary epithelial cells 16 . Deleterious mutations in SBK1 were pinpointed in the metastasis of acral melanoma by WES 17 .…”

“…15 SBK1 was also activated in the malignant transformation of pulmonary epithelial cells. 16 Deleterious mutations in SBK1 were pinpointed in the metastasis of acral melanoma by WES. 17 Li et al 18 identified that SBK1 was upregulated in ovarian cancer cells and was associated with poor prognosis.…”

A novel risk model of three gefitinib-related genes FBP1, SBK1 and AURKA is related to the immune microenvironment and is predicting prognosis of lung adenocarcinoma patients

“…In PC9 cells, Gefitinib could promote FBP1 expression and inhibit SBK1 and AURKA levels. An increasing body of evidence suggests that FBP1, SBK1 and AURKA are associated with lung cancer progression [10][11][12][13][14][15]. For instance, FBP1 was found to be downregulated in lung cancer tissues and cells.…”

“…Upon inhibition of long non-coding RNA ENST000001520 in BEAS-2B cells, a notable decrease is observed in cell migration and proliferation abilities, accompanied by an increase in apoptosis capabilities. These changes can be attributed to the downregulation of SBK1 and SOCS3 mRNA and protein expression [ 14 ]. This finding indicated that Gefitinib could affect FBP1, SBK1 and AURKA expression to inhibit LUAD progression.…”

Identification of a ZC3H12D-regulated competing endogenous RNA network for prognosis of lung adenocarcinoma at single-cell level

“…However, there was no significant difference in the expression of GNAO1 and KSR2 between tumor and paracancerous tissue samples. Meanwhile, both SBK1 and SLIT3 were found to play a vital role in lung cancer by miRNA or lncRNA [49][50][51]. Thereinto, we focused on ZC3H12D to construct a ceRNA regulatory network, and screened for potentially functional miRNAs and lncRNAs (Fig.…”