LncRNA EWSAT1 upregulates CPEB4 via miR-330-5p to promote cervical cancer development

Zhou, Qing-Yan; Xie, Yuan; Wang, Li; Xu, Tao; Gao, Yongbin

doi:10.1007/s11010-020-03778-8

Cited by 13 publications

(18 citation statements)

References 38 publications

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“… 39 In addition, CPEB4 has been reported to be upregulated in cervical cancer tissues, and EWSAT1 upregulated CPEB4 to promote cervical cancer development. 19 Consistently, we also observed that CPEB4 was upregulated in cervical cancer tissues and cells. Moreover, we also identified that overexpression of CPEB4 could attenuate the anti-cancer role of miR-758-3p cervical cancer cells.…”

Section: Discussionsupporting

confidence: 81%

“… 18 As a member of cytoplasmic polyadenylation element-binding protein (CPEB) family, CPEB4 has been revealed to be overexpressed in cervical cancer samples and involved in cervical cancer development. 19 Based on the analysis of bioinformatics software, circ_0003221 and CPEB4 were found to have complementary binding sites with miR-758-3p, which stimulated us to assume the ceRNA regulatory network of circ_0003221/miR-758-3p/CPEB4 in cervical cancer.…”

Section: Introductionmentioning

confidence: 99%

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Circular RNA Circ_0003221 Promotes Cervical Cancer Progression by Regulating miR-758-3p/CPEB4 Axis

Xie¹,

Wang²,

Wang³

2021

CMAR

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Background: Circular RNAs (circRNAs) play crucial roles in the development and progression of various cancers, including cervical cancer. However, the role and regulatory mechanism of circ_0003221 in cervical cancer are still unclear. Methods: The expression of circ_0003221, microRNA-758-3p (miR-758-3p), cytoplasmic polyadenylation element-binding protein 4 (CPEB4) was detected by quantitative real-time PCR (qRT-PCR). Cell Counting Kit-8 (CCK-8), colony formation, and 5-Ethynyl-2ʹdeoxyuridine (Edu) assays were utilized to determine cell proliferation. Cell cycle distribution was analyzed by flow cytometry. Cell migration and invasion were detected by transwell assay. All protein levels were detected by Western blot assay. The interaction between miR-758-3p and circ_0003221 or CPEB4 was confirmed by dual-luciferase reporter, RNA immunoprecipitation (RIP) and RNA pull-down assays. Mice xenograft model of cervical cancer was established to verify the function of circ_0003221 in vivo. Results: Circ_0003221 was upregulated in cervical cancer tissues and cells. Knockdown of circ_0003221 suppressed cell proliferation, migration, invasion, and EMT and induced cell cycle arrest in cervical cancer cells. MiR-758-3p was a direct target of circ_0003221, and miR-758-3p inhibition reversed the effects of circ_0003221 knockdown in cervical cancer cells. Moreover, CPEB4 was identified as a direct target of miR-758-3p, and miR-758-3p exerted its anti-cancer role by targeting CPEB4. Furthermore, circ_0003221 acted as a sponge of miR-758-3p to upregulate CPEB4 expression. In addition, circ_0003221 silence also suppressed tumor growth and EMT in vivo. Conclusion: Circ_0003221 knockdown inhibited cervical cancer progression via modulating miR-758-3p/CPEB4 axis, which might suggest a new insight into the pathogenesis of cervical cancer.

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Section: Discussionsupporting

confidence: 81%

Section: Introductionmentioning

confidence: 99%

Circular RNA Circ_0003221 Promotes Cervical Cancer Progression by Regulating miR-758-3p/CPEB4 Axis

Xie¹,

Wang²,

Wang³

2021

CMAR

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“…LINC00271 low expression was associated with poor prognosis in papillary thyroid cancer (25) and in adrenocortical tumors (26). EWSAT1 was associated with progression in several cancer types, such as ovarian (27), cervical (28) and colorectal (29).…”

Section: Discussionmentioning

confidence: 99%

Unraveling Immune-Related lncRNAs in Breast Cancer Molecular Subtypes

et al. 2021

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Breast cancer (BRCA) is the most leading cause of cancer worldwide. It is a heterogeneous disease with at least five molecular subtypes including luminal A, luminal B, basal-like, HER2-enriched, and normal-like. These five molecular subtypes are usually stratified according to their mRNA profile patterns; however, ncRNAs are increasingly being used for this purpose. Among the ncRNAs class, the long non-coding RNAs (lncRNAs) are molecules with more than 200 nucleotides with versatile regulatory roles; and high tissue-specific expression profiles. The heterogeneity of BRCA can also be reflected regarding tumor microenvironment immune cells composition, which can directly impact a patient’s prognosis and therapy response. Using BRCA immunogenomics data from a previous study, we propose here a bioinformatics approach to include lncRNAs complexity in BRCA molecular and immune subtype. RNA-seq data from The Cancer Genome Atlas (TCGA) BRCA cohort was analyzed, and signal-to-noise ratio metrics were applied to create these subtype-specific signatures. Five immune-related signatures were generated with approximately ten specific lncRNAs, which were then functionally analyzed using GSEA enrichment and survival analysis. We highlighted here some lncRNAs in each subtype. LINC01871 is related to immune response activation and favorable overall survival in basal-like samples; EBLN3P is related to immune response suppression and progression in luminal B, MEG3, XXYLT1-AS2, and LINC02613 were related with immune response activation in luminal A, HER2-enriched and normal-like subtypes, respectively. In this way, we emphasize the need to know better the role of lncRNAs as regulators of immune response to provide new perspectives regarding diagnosis, prognosis and therapeutical targets in BRCA molecular subtypes.

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“…For example, lncRNA PART1 inhibited miR-150-5p thereby upregulating LRG1 to promote colorectal cancer development. 8 SBF2-AS1 driven colorectal cancer via regulating miR-619-5p/HDAC3 axis. 15 Depletion of SNHG14 inhibited CRC cell proliferation in vitro by modulating miR-32-5p/SKIL axis.…”

Section: Introductionmentioning

confidence: 98%

“…EWSAT1 was first discovered to play roles in Ewing sarcoma development. 6 Thereafter, emerging evidence revealed that EWSAT1 was highly expressed and exerted oncogenic roles in a series of cancer types, such as nasopharyngeal carcinoma, 7 cervical cancer, 8 osteosarcoma, 9 and ovarian cancer. 10 However, the role of EWSAT1 in colorectal cancer was not elaborated.…”

Section: Introductionmentioning

confidence: 99%

LncRNA EWSAT1 Promotes Colorectal Cancer Progression Through Sponging miR-326 to Modulate FBXL20 Expression

Liu¹,

Huang²,

Liao³

et al. 2021

OTT

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Background: Ewing sarcoma-associated transcript 1 (EWSAT1) has been reported to be a pivotal modulator in a series of cancers. However, the function of EWSAT1 in colorectal cancer (CRC) has not been elaborated. This study aimed to explore the role of EWSAT1 in CRC progression and the underlying mechanisms. Methods: The expression patterns of EWSAT1, miR-326 and FBXL20 were examined by qCRCR. Si-EWSAT1 was transfected to study the effects of EWSAT1 on cell proliferation and metastasis. Rescue experiments were performed to investigate the underlying mechanisms in vitro. Xenograft models were used to evaluate the role of EWSAT1 in vivo. Results: We found that EWSAT1 was highly expressed in CRC tissues and cell lines and associated with poor overall survival. In vitro, knockdown of EWSAT1 suppressed the cell proliferation, migration and invasion. Moreover, miR-326 was found to be a target of EWSAT1, and miR-326 inhibitor could partially reverse the effects on CRC cell progression induced by si-EWSAT1. Subsequently, we validated FBXL20 as a vital downstream target for miR-326, and EWSAT1 positively regulated FBXL20 via miR-326 in vitro. In addition, these findings were confirmed by in vivo experiments. Conclusion:Taken together, the data showed that EWSAT1 promoted CRC progression via targeting miR-326/FBXL20 pathway, which might provide a novel therapeutic target for CRC treatment.

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LncRNA EWSAT1 upregulates CPEB4 via miR-330-5p to promote cervical cancer development

Cited by 13 publications

References 38 publications

Circular RNA Circ_0003221 Promotes Cervical Cancer Progression by Regulating miR-758-3p/CPEB4 Axis

Circular RNA Circ_0003221 Promotes Cervical Cancer Progression by Regulating miR-758-3p/CPEB4 Axis

Unraveling Immune-Related lncRNAs in Breast Cancer Molecular Subtypes

LncRNA EWSAT1 Promotes Colorectal Cancer Progression Through Sponging miR-326 to Modulate FBXL20 Expression

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