LncRNA expression in the spinal cord modulated by minocycline in a mouse model of spared nerve injury

Liu, Zihao; Liang, Ying; Wang, Honghua; Lu, Zhenhe; Chen, Jinsheng; Huang, Qingyang; Sheng, Lei; Ma, Yanxia; Du, Huiying; Gong, Qingjuan

doi:10.2147/jpr.s147055

Cited by 29 publications

(25 citation statements)

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“…Most of the studies focus on whole spinal cord tissues and therefore do not distinguish between alterations occurring in neuronal and non-neuronal cells [183,184,[205][206][207][208]. Nevertheless, the identification of deregulated lncRNAs and circRNAs and the computational construction of interaction-networks between lncRNAs/circRNAs-miRNAs-mRNAs provide directions for future studies and potential therapeutic targets.…”

Section: Lncrnas Deregulated In the Spinal Cord And Brainmentioning

confidence: 99%

“…Nevertheless, the identification of deregulated lncRNAs and circRNAs and the computational construction of interaction-networks between lncRNAs/circRNAs-miRNAs-mRNAs provide directions for future studies and potential therapeutic targets. Interestingly, not only SNI-induced mechanical hyperalgesia but also the differential expression of lncRNAs is alleviated by the tetracycline antibiotic minocycline [205]. Few lncRNAs have been functionally investigated at the spinal cord level.…”

Section: Lncrnas Deregulated In the Spinal Cord And Brainmentioning

confidence: 99%

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Non-coding RNAs in neuropathic pain

2020

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Neuro-immune alterations in the peripheral and central nervous system play a role in the pathophysiology of chronic pain in general, and members of the non-coding RNA (ncRNA) family, specifically the short, 22 nucleotide microRNAs (miRNAs) and the long non-coding RNAs (lncRNAs) act as master switches orchestrating both immune as well as neuronal processes. Several chronic disorders reveal unique ncRNA expression signatures, which recently generated big hopes for new perspectives for the development of diagnostic applications. lncRNAs may offer perspectives as candidates indicative of neuropathic pain in liquid biopsies. Numerous studies have provided novel mechanistic insight into the role of miRNAs in the molecular sequelae involved in the pathogenesis of neuropathic pain along the entire pain pathway. Specific processes within neurons, immune cells, and glia as the cellular components of the neuropathic pain triad and the communication paths between them are controlled by specific miRNAs. Therefore, nucleotide sequences mimicking or antagonizing miRNA actions can provide novel therapeutic strategies for pain treatment, provided their human homologues serve the same or similar functions. Increasing evidence also sheds light on the function of lncRNAs, which converge so far mainly on purinergic signalling pathways both in neurons and glia, and possibly even other ncRNA species that have not been explored so far.

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Section: Lncrnas Deregulated In the Spinal Cord And Brainmentioning

confidence: 99%

Section: Lncrnas Deregulated In the Spinal Cord And Brainmentioning

confidence: 99%

Non-coding RNAs in neuropathic pain

2020

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“…SNI surgeries were performed to mice according to our previous work [24]. In brief, mice were deeply anesthetized after the behavioral tests.…”

Section: Surgical Procedures and Drug Infusionmentioning

confidence: 99%

“…Total RNA was extracted using the Trizol method [24,27]. The quality and quantity of RNA were measured using a Nanodrop Spectrophotometer (Thermo Scienti c) and samples with an absorbance ratio at 260/280 between 1.8-2.2 were considered acceptable.…”

Section: Rna Extraction and Quantitative Real Time Pcr (Qpcr)mentioning

confidence: 99%

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MiR-106b-5p represses neuropathic pain by regulating P2X4 receptor in the spinal cord in mice

Tan

Wei

et al. 2020

Preprint

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Background: P2X4 receptor (P2X4R)-mediated spinal microglial activation makes a critical contribution to pathologically enhanced pain processing in the dorsal horn. It can be upregulated under conditions of neuropathic pain. However, the specific mechanism of pathogenesis and potential molecular targets has not yet been made explicit. MicroRNAs (miRNAs) are commonly recognized as indicators in neuropathic pain pathophysiology.Methods: We established the pain model of spared nerve injury (SNI), and the 50% paw withdrawal thresholds (PWMTs) were used to assess behavior of mouse. MiRNA expression profiling was performed to detect differential expressed miRNA. The western-bolt and quantitative real time PCR to examine P2X4R and miRNA expression in the mouse. Dual-luciferase reporter assays confirmed the correlation between P2X4R and miRNA. Fluorescence in situ hybridization was used to show location between P2X4R and miRNA. Results: In the present study, we found that P2X4R was up-regulated in the spinal dorsal horn of mice following spared nerve injury (SNI), and we identified 69 miRNAs (46 up-regulated and 23 down-regulated miRNAs) were differently expressed (fold change > 2, P < 0.05). P2X4R was a major target of miR-106b-5p (one of down-regulated miRNAs in SNI) with bioinformatics technology and quantitative real time PCR analysis validated the expressed change of miR-106b-5p, and dual-luciferase reporter assays confirmed the correlation between them. Fluorescence in situ hybridization showed that miR-106b-5p was co-localized with P2X4R in the spinal cord. Transfection with miR-106b-5p mimic on BV2 cells reversed the up-regulation of P2X4R induced by LPS. Moreover, miR-106b-5p overexpression significantly attenuated neuropathic pain induced by SNI, with decreased expression of P2X4R mRNA and protein in the spinal cord.Conclusion: Taken together, our results suggest that miR-106b-5p can serve as an important regulator of neuropathic pain development by targeting P2X4R.

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Identification and coregulation pattern analysis of long noncoding RNAs following subacute spinal cord injury

Wang

et al. 2021

Journal Orthopaedic Research

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Long noncoding RNAs (lncRNAs) have been demonstrated to play critical regulatory roles in posttranscriptional and transcriptional regulation in eukaryotic cells.However, the characteristics of many lncRNAs, particularly their expression patterns in the lesion epicenter of spinal tissues following subacute spinal cord injury (SCI), remain unclear. In this study, we determined the expression profiles of lncRNAs in the lesion epicenter of spinal tissues after traumatic SCI and predicted latent regulatory networks. Standard Allen's drop surgery was conducted on mice, and hematoxylin and eosin staining was used to observe the damaged area. Highthroughput sequencing was performed to identify the differential expression profiles of lncRNAs. Quantitative real-time polymerase chain reaction was conducted to evaluate the quality of the sequencing results. Bioinformatics analyses, including Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analysis, coexpression analysis, and protein-protein interaction analysis, were performed.Targeted binding of lncRNA-miRNA-mRNA was predicted by TargetScan and miRanda. A total of 230 differentially expressed lncRNAs were identified and preliminarily verified, and some potential regulatory networks were constructed. These findings improve our understanding of the mechanisms underlying subacute SCI; differentially expressed lncRNAs are closely involved in pathophysiological processes by regulating multiple pathways. Further studies are essential for revealingThis is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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LncRNA expression in the spinal cord modulated by minocycline in a mouse model of spared nerve injury

Cited by 29 publications

References 50 publications

Non-coding RNAs in neuropathic pain

Non-coding RNAs in neuropathic pain

MiR-106b-5p represses neuropathic pain by regulating P2X4 receptor in the spinal cord in mice

Identification and coregulation pattern analysis of long noncoding RNAs following subacute spinal cord injury

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