LncRNA expression profiles of EGFR exon 19 deletions in lung adenocarcinoma ascertained by using microarray analysis

Abstract

Studies showed that long chain non-coding RNAs (lncRNAs) involved in the development and progression of lung cancer. However, the mechanisms of EGFR exon 19 deletion in lung adenocarcinoma were unclear. Lung adenocarcinoma was divided into EGFR exon 19 deletion group and EGFR wild-type group. We studied the differential expression profiles of lncRNAs in EGFR exon 19 deletion in lung adenocarcinoma by high-throughput microarray. Using abundant and varied probes, we were able to assess 30,586 lncRNAs and 26,109 mRNAs in our microarray. Compared with the wild-type EGFR, we found that 1,533 lncRNAs and 1,406 mRNAs were differentially expressed (≥ twofold change) in EGFR exon 19 deletion in lung adenocarcinoma, indicating that many lncRNAs were significantly upregulated or downregulated in EGFR exon 19 deletion in lung adenocarcinoma. The 10 lncRNAs were aberrantly expressed in EGFR exon 19 deletion in lung adenocarcinoma compared with wild-type EGFR group validated by real-time RT-PCR. Among these, RP11-325I22.2 and LOC440905 were the most aberrantly expressed in 20 cases of EGFR exon 19 deletion in lung adenocarcinoma samples by real-time RT-PCR. Our study showed lncRNAs expression pattern in EGFR exon 19 deletion in lung adenocarcinoma by microarray. RP11-325I22.2 and LOC440905 might play an important role in the mechanism of EGFR exon 19 deletion in lung adenocarcinoma. The study may provide a new mechanism of EGFR exon 19 deletion in lung adenocarcinoma.

Long Noncoding RNAs in Lung Cancer

“…Similar to previous studies, BANCR was also suggested to influence cell migration in colorectal cancer, probably via modulation of EMT (Guo et al 2014). In papillary thyroid carcinoma, BANCR knockdown reduced cell proliferation and moderately induced apoptosis in a cell line (Wang et al 2014d). No effect on cellular metastatic potential was observed.…”

“…In contrast to NSCLC, BANCR was significantly upregulated in malignant melanoma, colorectal carcinoma, and papillary thyroid carcinoma tissue (Wang et al 2014d;Guo et al 2014;Flockhart et al 2012). In the original study on BANCR, an influence of the lncRNA on melanoma cell migration via regulation of CXCL11 chemokine expression levels was observed (Flockhart et al 2012).…”

“…No effect on cellular metastatic potential was observed. Furthermore, initial attempts to concretize BANCR function in papillary thyroid carcinoma hint at the regulation of autophagy-related processes (Wang et al 2014d). …”

Functions of lncRNA HOTAIR in lung cancer

“…lncRNAs have emerged as novel master regulators of initiation, progression, and response to therapy in a wide variety of solid tumors and hematological malignancies [ 6 ],[ 7 ]. Hundreds of IncRNAs have been associated with lung cancer through gene expression microarrays and massively parallel RNA sequencing of tumor tissues and paired adjacent non-tumor tissues in the lung [ 8 ]-[ 11 ]. As of September 2014, a PubMed search using lncRNA and lung cancer as key words yielded more than a dozen of lncRNAs that have been individually investigated in lung cancer (Table 1 ) [ 8 ],[ 12 ]-[ 38 ].…”

Intersecting transcriptomic profiling technologies and long non-coding RNA function in lung adenocarcinoma: discovery, mechanisms, and therapeutic applications

“…Illumina-developed arrays contained less information on lncRNAs due to their design emphasis on 3′UTR targeting, however several lncRNAs were included under the ‘LOC_” definition. Using this probe-based approach several studies were able to identify lncRNA expression profiles in lung cancer and perform preliminary analysis [ 136 – 139 ], the results of which have been conveniently collated by lnc2cancer [ 140 ]. However, the discernable drawbacks of such techniques include the lack of discovery and low-expression levels of lncRNAs, thwarting detection efforts.…”