lncRNA FAM83H‑AS1 is associated with the prognosis of colorectal carcinoma and promotes cell proliferation by targeting the Notch signaling pathway

Song, Lingyun; Dong, Wei; Zhao, Pei; Liu, Zhenjun

doi:10.3892/ol.2017.7520

Cited by 37 publications

(58 citation statements)

References 34 publications

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“…FAM83H-AS1 was consistently over expressed in breast tumor samples and overall survival analysis of TCGA data sets showed poor prognosis of the up regulated group which are in agreement with other studies in breast, colorectal and lung cancer (Lu et al, 2018;Yang et al, 2016a;Yang et al, 2016c;Zhang et al, 2017). Functional studies have demonstrated that knock-down of FAM83H-AS1 proliferative potential through MET/EGFR signaling in lung adenocarcinoma and NOTCH1 signaling pathway in colorectal cancer.…”

Section: Discussionsupporting

confidence: 89%

Identification of lncRNAs associated with early stage breast cancer and their prognostic implications

Kuha

Patel

et al. 2019

Preprint

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KeywordsLong non-coding RNAs; Breast cancer; ADAMTS9-AS2; FAM83H-AS1; RNA sequencing; ncRNAs Abbreviations DCIS -ductal carcinoma in situ IDC -invasive ductal carcinoma NGS -Next generation sequencing lncRNA -long non-coding RNAs lincRNA -long intergenic non-coding RNA TNBC -Triple negative breast cancers BH -Bonferroni and Benjamini-Hochberg PCA -Principal component analysis PCC -Pearson's correlation coefficient PCG-Protein coding genes AbstractBreast cancer is a common malignancy among women with the highest incidence rate worldwide. Dysregulation of long non-coding RNAs occurring in the preliminary stages of breast carcinogenesis is poorly understood. In this study, RNA sequencing was done to identify long non-coding RNA expression profiles associated with early-stage breast cancer.RNA sequencing was done in 6 invasive ductal carcinoma (IDC) tissues along with paired normal tissue samples, 7 ductal carcinoma in situ (DCIS) tissues and 5 apparently normal breast tissues. We identified 375 differentially expressed lncRNAs (DElncRNAs) in IDC tissues compared to paired normal tissues. Antisense transcripts (~58%) were the largest subtype among DElncRNAs. About 20% of the 375 DElncRNAs were supported by typical split readings leveraging their detection confidence. Validation was done in n=52 IDC and paired normal tissue by qRT-PCR for the identified targets (ADAMTS9-AS2, EPB41L4A-AS1, WDFY3-AS2, RP11-295M3.4, RP11-161M6.2, RP11-490M8.1, CTB-92J24.3 and FAM83H-AS)1. We evaluated the prognostic significance of DElncRNAs based on TCGA datasets and overexpression of FAM83H-AS1 was associated with patient poor survival. We confirmed that the down-regulation of ADAMTS9-AS2 in breast cancer was due to promoter hypermethylation through in-vitro silencing experiments and pyrosequencing.

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Section: Discussionsupporting

confidence: 89%

Identification of lncRNAs associated with early stage breast cancer and their prognostic implications

Kuha

Patel

et al. 2019

Preprint

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“…LncRNA H19 has been reported to promote the apoptosis of hippocampal neurons by inhibiting let‐7b . LncRNA UCA1 inhibits seizures and brain damage caused by epilepsy by modulating the miR‐495/Nrf2‐ARE signaling pathway …”

Section: Discussionmentioning

confidence: 99%

“…24 LncRNA UCA1 inhibits seizures and brain damage caused by epilepsy by modulating the miR-495/Nrf2-ARE signaling pathway. 25 LncRNA GAS5 plays an important regulatory role in cancer progression, and it can inhibit oncogenes by inhibiting tumor cell proliferation, promoting apoptosis, and inhibiting cell migration and invasion. 26 Thus, we set out to establish a rat epilepsy model as well as a cell epilepsy model in order to detect the expression levels of IncRNA GAS5.…”

Section: Discussionmentioning

confidence: 99%

Long noncoding RNA GAS5 silencing inhibits the expression of KCNQ3 by sponging miR‐135a‐5p to prevent the progression of epilepsy

Zheng

et al. 2019

The Kaohsiung J of Med Scie

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Epilepsy is one of the most common neurological disorders in humans. Recently, long noncoding RNAs (lncRNAs) have been reported to be important players in neurological diseases. Herein, this study aimed to examine the effect of lncRNA GAS5 on the occurrence of epilepsy in rat and cell models of epileptic seizure. The expression of lncRNA GAS5 was measured in the established rat and cell models. The binding sites between lncRNA GAS5 and miR‐135a‐5p, as well as those between miR‐135a‐5p and 3′ untranslated region of KCNQ3 were predicted by miRDB and Targetscan, separately, followed by verification using dual‐luciferase reporter gene assay. The expression of miR‐135a‐5p was measured in response to the overexpression of lncRNA GAS5. The mRNA and protein levels of KCNQ3 were examined in response to overexpression of miR‐135a‐5p. Next, the latency of epilepsy and frequency of epileptic seizures were assessed in rats injected with Lv‐shGAS5 and Lv‐miR‐135a‐5p in epileptic seizure model. In the rat and cell models, lncRNA GAS5 was highly expressed when epileptic seizure was induced. The expression of miR‐135a‐5p was decreased by overexpression of lncRNA GAS5. Meanwhile, the mRNA and protein levels of KCNQ3 were decreased in response to knockdown of miR‐135a‐5p. After the treatment of Lv‐shGAS5 and Lv‐miR‐135a‐5p, the average latent period of epilepsy was prolonged and the frequency of seizures was decreased. The key findings of the present study provide evidence emphasizing that lncRNA GAS5 functions as a competitive endogenous RNA of miR‐135a‐5p to increase expression of KCNQ3, and lncRNA GAS5 silencing inhibited the occurrence and progression of epilepsy.

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“…In recent years, more and more studies focused on implicating the role of lncRNAs in diseases including cancers [25][26][27]. Some studies had reproted that lncRNA FAM83H-AS1 was associated with many types of cancers such as breast cancer [28], colorectal cancer [29] and colon cancer [30]. However, the function and mechanism of FAM83H-AS1 in OC has not been systemically studied.…”

Section: Discussionmentioning

confidence: 99%

Long non-coding RNA FAM83H-AS1 acts as an oncogenic driver in human ovarian cancer

Yuan

Huang

Guo

et al. 2020

Preprint

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Background Ovarian cancer (OC) is one of the most aggressive women cancers with increasing incidence and mortality rates worldwide. Long non-coding RNAs (lncRNAs) could as major players in OC process. Although FAM83H antisense RNA1 (FAM83H-AS1) is demonstrated play an important roles in a many cancers, the detailed function and mechanism has not been reported in OC. Methods We integrated multiple kinds of bioinformatics approaches and experiments validated method to evaluate functions of FAM83H-AS1 in OC. Results Some differential expressed lncRNAs were identified between OC and normal control tissues. FAM83H-AS1 was one of most differentially expressed lncRNAs and up-regulated in multiple cancer types. Specially, expression of FAM83H-AS1 was higher in OC and showed difference in diverse stages. High FAM83H-AS1 expression is associated with worse pan-cancer and OC outcomes. FAM83H-AS1-centric network including lncRNA-miRNA, lncRNA-protein and lncRNA-mRNA ceRNA network were constructed to infer the function and mechanism of FAM83H-AS1. There were two methylation sites including cg01399317 and cg20519035 located at FAM83H-AS1. The methylation level of cg01399317 was correlated with gene expression of FAM83H-AS1. The expression level of FAM83H-AS1 was correlated with infiltration level of immune cell including macrophage, neutrphil and dendritic cell in OC patients. Lastly, qRT-PCR showed that the expression of FAM83H-AS1 was higher in OC tissues than normal control tissues. Conclusions Collectively, these results indicated that FAM83H-AS1 may act as an oncogenic driver and it may be a potential therapy target in OC.

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lncRNA FAM83H‑AS1 is associated with the prognosis of colorectal carcinoma and promotes cell proliferation by targeting the Notch signaling pathway

Cited by 37 publications

References 34 publications

Identification of lncRNAs associated with early stage breast cancer and their prognostic implications

Identification of lncRNAs associated with early stage breast cancer and their prognostic implications

Long noncoding RNA GAS5 silencing inhibits the expression of KCNQ3 by sponging miR‐135a‐5p to prevent the progression of epilepsy

Long non-coding RNA FAM83H-AS1 acts as an oncogenic driver in human ovarian cancer

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