LncRNA FENDRR in Carcinoma-Associated Fibroblasts Regulates the Angiogenesis of Oral Squamous Cell Carcinoma Through the PI3K/AKT Pathway

Xu, Yuming; Jiang, Erhui; Shao, Zhang; Shang, Zhengjun

doi:10.3389/fonc.2021.616576

Cited by 4 publications

(2 citation statements)

References 31 publications

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“…In this work, we observed that COL5A1 knockdown suppresses proliferation, migration, invasiveness, and apoptosis resistance of OSCC cells. Moreover, the COL5A1 downregulation also suppressed the angiogenesis activity, which represents a critical factor linking to tumor growth, metastasis, and poor prognosis of patients 21 . In addition, we found that the COL5A1 knockdown limited the growth and metastasis of xenograft tumors formed by OSCC cells.…”

Section: Discussionmentioning

confidence: 62%

“…Moreover, the COL5A1 downregulation also suppressed the angiogenesis activity, which represents a critical factor linking to tumor growth, metastasis, and poor prognosis of patients. 21 In addition, we found that the COL5A1 knockdown limited the growth and metastasis of xenograft tumors formed by OSCC cells. These results reveal the oncogenic role of COL5A1 in OSCC.…”

Section: Discussionmentioning

confidence: 78%

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LINC00173 blocks GATA6‐mediated transcription of COL5A1 to affect malignant development of oral squamous cell carcinoma

Chen

Zhao

Yang

et al. 2023

J Oral Pathology Medicine

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Background Aberrant expression of collagen type V alpha 1 chain (COL5A1) has been linked to several forms of human cancers. In this work, we focused on the interaction of the LINC00173/GATA binding protein 6 (GATA6)/COL5A1 axis in the malignant property of oral squamous cell carcinoma (OSCC) cells. Methods We analyzed six publicly accessible datasets GSE160042, GSE74530, GSE138206, GSE23558, GSE31853 and GSE146483 to identify aberrantly expressed genes in OSCC. The expression of COL5A1 in OSCC tissues and cell lines was examined by reverse transcription‐quantitative polymerase chain reaction and/or immunohistochemistry. The regulatory mechanism responsible for COL5A1 transcription was predicted via bioinformatics systems, and the interactions of LINC00173, GATA6, and COL5A1 were identified by immunoprecipitation and luciferase assays. Overexpression or downregulation of COL5A1, GATA6, and LINC00173 were induced in OSCC cell lines to determine their roles in the malignant phenotype of the OSCC cells in vitro and in vivo. Results COL5A1 showed elevated expression in OSCC tissues and cells. The COLA51 knockdown suppressed proliferation, migration and invasiveness, apoptosis resistance, and pro‐angiogenic ability of OSCC cells, and it suppressed the growth and dissemination of xenograft tumors in vivo. GATA6 bound to COL5A1 promoter to activate its transcription, whereas LINC00173 bound to GATA6 to block this transcriptional activation. Overexpression of GATA6 or COL5A1 promoted the malignant phenotype of the OSCC cells, which were blocked upon LINC00173 upregulation. Conclusion This work demonstrates that LINC00173 blocks GATA6‐mediated transcription of COL5A1 to affect malignant development of OSCC.

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Section: Discussionmentioning

confidence: 62%

Section: Discussionmentioning

confidence: 78%

LINC00173 blocks GATA6‐mediated transcription of COL5A1 to affect malignant development of oral squamous cell carcinoma

Chen

Zhao

Yang

et al. 2023

J Oral Pathology Medicine

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Cancer-associated fibroblast-derived exosome microRNA-21 promotes angiogenesis in multiple myeloma

Miaomiao,

Xiaoqian,

Yuwei

et al. 2023

Sci Rep

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Multiple myeloma (MM) is the second most common hematological malignancy, and angiogenesis determines its progression. In the tumor microenvironment, normal fibroblasts (NFs) are transformed into cancer-associated fibroblasts (CAFs), which can promote angiogenesis. Microribonucleic acid-21 (miR-21) is highly expressed in various tumors. However, research on the relationship between tumor angiogenesis and miR-21 is rare. We analyzed the relationship between miR-21, CAFs, and angiogenesis in MM. NFs and CAFs were isolated from the bone marrow fluids of patients with dystrophic anemia and newly-diagnosed MM. Co-culturing of CAF exosomes with multiple myeloma endothelial cells (MMECs) showed that CAF exosomes were able to enter MMECs in a time-dependent manner and initiate angiogenesis by promoting proliferation, migration, and tubulogenesis. We found that miR-21 was abundant in CAF exosomes, entering MMECs and regulating angiogenesis in MM. By transfecting NFs with mimic NC, miR-21 mimic, inhibitor NC, and miR-21 inhibitor, we found that miR-21 significantly increased the expression of alpha-smooth muscle actin and fibroblast activation protein in NFs. Our results showed that miR-21 can transform NFs into CAFs, and that CAF exosomes promote angiogenesis by carrying miR-21 into MMECs. Therefore, CAF-derived exosomal miR-21 may serve as a novel diagnostic biomarker and therapeutic target for MM.

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Non-Coding RNAs in Oral Cancer: Emerging Roles and Clinical Applications

Dey,

Biswas,

Manoj Appadan

et al. 2023

Cancers

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Oral cancer (OC) is among the most prevalent cancers in the world. Certain geographical areas are disproportionately affected by OC cases due to the regional differences in dietary habits, tobacco and alcohol consumption. However, conventional therapeutic methods do not yield satisfying treatment outcomes. Thus, there is an urgent need to understand the disease process and to develop diagnostic and therapeutic strategies for OC. In this review, we discuss the role of various types of ncRNAs in OC, and their promising clinical implications as prognostic or diagnostic markers and therapeutic targets. MicroRNA (miRNA), long ncRNA (lncRNA), circular RNA (circRNA), PIWI-interacting RNA (piRNA), and small nucleolar RNA (snoRNA) are the major ncRNA types whose involvement in OC are emerging. Dysregulated expression of ncRNAs, particularly miRNAs, lncRNAs, and circRNAs, are linked with the initiation, progression, as well as therapy resistance of OC via modulation in a series of cellular pathways through epigenetic, transcriptional, post-transcriptional, and translational modifications. Differential expressions of miRNAs and lncRNAs in blood, saliva or extracellular vesicles have indicated potential diagnostic and prognostic importance. In this review, we have summarized all the promising aspects of ncRNAs in the management of OC.

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LncRNA FENDRR in Carcinoma-Associated Fibroblasts Regulates the Angiogenesis of Oral Squamous Cell Carcinoma Through the PI3K/AKT Pathway

Cited by 4 publications

References 31 publications

LINC00173 blocks GATA6‐mediated transcription of COL5A1 to affect malignant development of oral squamous cell carcinoma

LINC00173 blocks GATA6‐mediated transcription of COL5A1 to affect malignant development of oral squamous cell carcinoma

Cancer-associated fibroblast-derived exosome microRNA-21 promotes angiogenesis in multiple myeloma

Non-Coding RNAs in Oral Cancer: Emerging Roles and Clinical Applications

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