LncRNA FLVCR1-AS1 mediates miR-513/YAP1 signaling to promote cell progression, migration, invasion and EMT process in ovarian cancer

Yan, Huan; Li, Hong; Silva, Maria A.; Guan, Yichun; Li, Yang; Zhu, Linlin; Zhang, Zhan; Li, Genxia; Ren, Chen

doi:10.1186/s13046-019-1356-z

Cited by 71 publications

(54 citation statements)

References 23 publications

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“…LncRNA NR_038323 suppresses the activity of miR‐324‐3p to up‐regulate DUSP1 with respect to suppressing renal fibrosis in diabetic nephropathy . LncRNA FLVCR1‐AS1 attenuates the suppression of miR‐513 on YAP1 activity in ovarian cancer . It is worth noting that the central role of lncRNAs or miRNAs has not been well investigated both functionally and mechanically, despite the strong indication of their importance in human diseases.…”

Section: Introductionmentioning

confidence: 99%

miR‐142‐5p protects against osteoarthritis through competing with lncRNA XIST

Sun

et al. 2020

The Journal of Gene Medicine

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Background The relevance between abnormal microRNA expression and osteoarthritis (OA) has been elaborated in recent studies. Hence, the present study aimed to assess the impact of miR‐142‐5p on chondrocyte growth and apoptosis. Methods To mimic OA‐like chondrocyte damage, interleukin (IL)‐1β was used for chondrocyte treatment. The expression of miR‐142‐5p, SGTB, long non‐coding RNA (lncRNA) X inactive specific transcript (XIST) and involved molecules such as Col2A1, Bcl‐2, MMP13 and Bax was determined via a quantitative reverse transcriptase‐polymerase chain reaction and western blot analyses. Functional roles of miR‐142‐5p, SGTB and XIST were monitored in 5‐ethynyl‐2'‐deoxyuridine, CCK‐8 and TUNEL experiments. Rescue analyses were conducted to consolidate the effect of the XIST/miR‐142‐5p/SGTB axis on chondrocytes in OA. Results miR‐142‐5p was down‐regulated in IL‐1β‐treated chondrocytes, whereas SGTB and XIST levels were increased. Overexpression of miR‐142‐5p stimulated proliferation and retarded apoptosis in IL‐1β‐treated chondrocytes. Meanwhile, miR‐142‐5p elevation was correlated with an elevation of Col2A1 and Bcl‐2, as well as a decline of MMP13 and Bax. A mechanistic study showed that miR‐142‐5p negatively regulated SGTB expression. Moreover, we found that lncRNA XIST could relieve the inhibition of miR‐142‐5p on SGTB expression. Augmentation of SGTB or suppression of miR‐142‐5p reversed the influence of XIST depletion on chondrocyte growth and apoptosis. Conclusions The present study has explored the fundamental role of miR‐142‐5p in IL‐1β‐treated chondrocytes, as well as the novel molecular mechanism constituted by miR‐142‐5p/SGTB/XIST in OA. Potentially, the results obtained may add new insight into OA pathogenesis.

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Section: Introductionmentioning

confidence: 99%

miR‐142‐5p protects against osteoarthritis through competing with lncRNA XIST

Sun

et al. 2020

The Journal of Gene Medicine

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“…MicroRNAs (miRNAs) are small RNAs (< 200 nt) that are known to repress the expression of their target genes by interacting with their binding sites on the target gene mRNAs and suppressing protein translation [6], and lncRNAs could regulate the expression of miRNA target genes by competitively binding to these miRNAs [7] . LncRNAs including FLVCR1-AS1, HOXD-AS1, MLK7-AS1 and HOTAIR [8][9][10][11] have been reported to promote ovarian cancer development through such mechanisms, while some other lncRNAs, such as MAGI2-AS3 [12], showed a cancer-suppressive role in ovarian cancer development.…”

Section: Introductionmentioning

confidence: 99%

LBX2-AS1 Promotes Ovarian Cancer Progression by Facilitating E2F2 Gene Expression via MIR-455-5P and MIR-491-5P Sponging

Cao

Wang

Tang

et al. 2020

Preprint

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Background:LBX2-AS1 is a long noncoding RNA that facilitates the development of gastrointestinal cancers and lung cancer, but its participation in ovarian cancer development remained uninvestigated.Methods: Clinical data retrieved from TCGA ovarian cancer database and the clinography of 60 ovarian cancer patients who received anti-cancer treatment in our facility were analyzed. The overall cell growth, colony formation, migration, invasion, apoptosis and tumor formation on nude mice of ovarian cancer cells were evaluated before and after lentiviral-based LBX2-AS1 knockdown. ENCORI platform was used to explore LBX2-AS1-interacting microRNAs and target genes of the candidate microRNAs. Luciferase reporter gene assay and RNA-pulldown assay were used to verify the putative miRNA-RNA interactions.Results: Ovarian cancer tissue specimens showed significant higher LBX2-AS1 expression levels that non-cancerous counterparts. High expression level of LBX2-AS1 significantly associated with patients reduced overall survival. LBX2-AS1 knockdown significantly downregulated the cell growth, colony formation, migration, invasion and tumor formation capacity of ovarian cancer cells and increased their apoptosis in vitro. LBX2-AS1 interacts with and thus inhibits the function of miR-455-5p and miR-491-5p, both of which restrained the expression of E2F2 gene in ovarian cancer cells via mRNA targeting. Transfection of miRNA inhibitors of these two miRNAs or forced expression of E2F2 counteracted the effect of LBX2-AS1 knockdown on ovarian cancer cells.ConclusionsLBX2-AS1 was a novel cancer-promoting lncRNA in ovarian cancer. This lncRNA increased the cell growth, survival, migration, invasion and tumor formation of ovarian cancer cells by inhibiting miR-455-5p and miR-491-5p, thus liberating the expression of E2F2 cancer-promoting gene.

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“…In total, 75 potentially relevant records were obtained. After excluding the duplicated and unqualified papers, 13 studies involving 914 patients with 8 different types of cancers were enrolled in this meta-analysis ultimately [5,10,13,14,[17][18][19][20][21][22][23][24][25]. These included studies comprised renal cell carcinoma [10], glioma [14,18], hepatocellular carcinoma [17], colorectal cancer [13,20,21,24], ovarian clear cell carcinoma [19], gastric cancer [11], esophageal squamous cell carcinoma [11] and osteosarcoma [23,25].…”

Section: Study Selection and Characteristicsmentioning

confidence: 99%

Clinicopathological and Prognostic Value of SNHG6 in Cancers: a Systematic Review and a Meta-analysis

Zhang

Qiu

Xie

et al. 2020

Preprint

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Background The long non-coding RNA small nucleolar RNA host gene 16 (lncRNA SNHG6) is dysregulated in various malignant tumor. However, a definite conclusion on the clinical value of lncRNA SNHG6 expression in human cancers has not been determined. The purpose of the present meta-analysis was to comprehensively elucidate the association between SNHG6 expression and clinical outcomes in cancers. Methods A systematic search was performed through the PubMed, Web of Science, Chinese National Knowledge Infrastructure (CNKI), and Wangfang databases for relevant studies. The pooled hazard ratios (HRs) with 95% confidence intervals (CIs) were collected to estimate the prognostic value, and the odds ratios (ORs) with 95% CIs were used to evaluate the relationship between lncRNA SNHG6 expression and clinicopathological features, including tumor invasion depth, lymph node metastasis (LNM), distance metastasis (DM), and TNM stage. Results A total of 914 patients from 13 studies were included in this meta-analysis. The pooled results suggested that evaluated SNHG6 expression could predict an unfavorable overall survival (OS) (HR = 2.04, 95% CI:1.56~2.52) with no heterogeneity ( I 2 = 0.0%, p = 0.996). Subgroup analysis indicated a significant association between high SNHG6 expression and shorter OS in studies with digestive system cancers (HR = 2.05, 95%CI: 1.47-2.62), sample size < 70 (HR = 2.70, 95%CI: 1.29-4.11), and univariate and multivariate analysis (HR = 2.04, 95%CI: 1.44-2.64). Moreover, high SNHG6 expression was positively correlated with tumor invasion depth (OR = 1.76, 95%CI: 1.18-2.63), LNM (OR = 1.60, 95%CI: 1.18-2.17), DM (OR = 1.90, 95%CI: 1.37-2.64) and advanced TNM stage (OR = 1.88, 95%CI: 1.36-2.60) in patients with cancers. Conclusions High lncRNA SNHG6 expression was correlated with tumor invasion depth, LNM, DM, and advanced TNM stage, suggesting that SNHG6 may serve as a promising prognostic biomarker of human cancers.

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LncRNA FLVCR1-AS1 mediates miR-513/YAP1 signaling to promote cell progression, migration, invasion and EMT process in ovarian cancer

Cited by 71 publications

References 23 publications

miR‐142‐5p protects against osteoarthritis through competing with lncRNA XIST

miR‐142‐5p protects against osteoarthritis through competing with lncRNA XIST

LBX2-AS1 Promotes Ovarian Cancer Progression by Facilitating E2F2 Gene Expression via MIR-455-5P and MIR-491-5P Sponging

Clinicopathological and Prognostic Value of SNHG6 in Cancers: a Systematic Review and a Meta-analysis

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