LncRNA GATA6-AS inhibits cancer cell proliferation and promotes cancer cell apoptosis in cervical cancer by down-regulating miR-205

Abstract

Background Dysregulated endothelial cell growth is involved in many types of human cancer, including cervical cancer. LncRNA GATA6-AS was reported to regulate endothelial cell growth, suggesting it might involve in cervical cancer. Our study was carried out to explore the involvement of GATA6-AS in cervical squamous cell carcinoma (CSCC), a subtype of cervical cancer. Methods To explore the expression of GATA6-AS, RT-qPCR was performed to detect GATA6-AS in plasma of 65 CSCC patients and 58 healthy females. To detect the expression of GATA6-AS, total RNAs were extracted. Results We found that plasma GATA6-AS expression was down-regulated in CSCC patients than that in healthy females, and HPV infection did not significantly affect the plasma expression of GATA6-AS. Moreover, we found that plasma GATA6-AS showed diagnostic values for CSCC by performing ROC curve analysis. The expression of miR-205 in plasma was also found to be up-regulated in CSCC patients than that in healthy females and inversely correlated with the expression of GATA6-AS in CSCC patients. Furthermore, over-expression of miR-205 did not significantly affect the expression of GATA6-AS in CSCC cells, while over-expression of GATA6-AS down-regulated miR-205 expression. In addition, GATA6-AS over-expression inhibited CSCC cell proliferation and promoted CSCC cell apoptosis, while miR-205 over-expression played opposite roles and attenuated the effects of GATA6-AS over-expression on CSCC cells. Conclusion Taken together, these results suggest that GATA6-AS may inhibit cell proliferation and promote cell apoptosis in CSCC by down-regulating miR-205.

LncRNA LAMTOR5‐AS1 sponges miR‐210‐3p and regulates cervical cancer progression

“…(5) Patients were monitored for five consecutive years through regular review, telephone interviews, family follow-up, and other measures; (6) The obtained cervical cancer tissue samples have been verified by pathologists.…”

“…4 With the promotion of cervical cancer screening and HPV vaccine, the incidence of cervical cancer has gradually decreased. 5,6 It is worth noting that today's young people have risky behaviors such as early sexual life, increased and uncertain sexual partners, and insecure sexual life. Global data show that the average age of onset of new cervical cancer patients is decreasing and the patients are getting younger.…”

“…It is known that persistent infection of high‐risk HPV is a major risk factor for cervical cancer 4 . With the promotion of cervical cancer screening and HPV vaccine, the incidence of cervical cancer has gradually decreased 5,6 . It is worth noting that today's young people have risky behaviors such as early sexual life, increased and uncertain sexual partners, and insecure sexual life.…”

A Novel Identified Long Non-coding RNA, lncRNA MEF2C-AS1, Inhibits Cervical Cancer via Regulation of miR-592/RSPO1

“…For example, Fan et al ( Fan et al, 2020 ) proved that LncRNA PTENP1 inhibited CC development by regulating miR-106b. Zhao et al ( Zhao et al, 2020 ) depicted that LncRNA GATA6-AS inhibited CC cells proliferation and promoted apoptosis by regulating miR-205. LncRNA MEF2C-AS1 is a novel identified long non-coding RNA.…”