LncRNA Gm4419 contributes to OGD/R injury of cerebral microglial cells via IκB phosphorylation and NF-κB activation

Wen, Yuanchao; Yu, Yunhu; Fu, Xiaohong

doi:10.1016/j.bbrc.2017.05.005

Cited by 67 publications

(45 citation statements)

References 22 publications

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“…Antisense non‐coding RNA in the cyclin‐dependent kinase inhibitor 4 locus (ANRIL), an antisense lncRNA co‐clustered with p15/CDKN2B‐p16/CDKN2A‐p14/ARF, is overexpressed in cerebral infarction rat models and plays a pro‐inflammatory role by activating NF‐κB pathway . Likewise, lncRNA Gm4419 could activate NF‐κB pathway and contributes to cell damage in oxygen‐glucose–deprived cerebral microglial cells . Another in vitro and in vivo study discloses that lncRNA SNHG14 elevates the expression of pro‐inflammatory factors (such as TNF‐α and nitric oxide), thereby aggravating neuron damage by regulating miR‐145‐5p/PLA2G4A .…”

Section: Discussionmentioning

confidence: 84%

“…24 Likewise, ln-cRNA Gm4419 could activate NF-κB pathway and contributes to cell damage in oxygen-glucose-deprived cerebral microglial cells. 25 Another in vitro and in vivo study discloses that lncRNA SNHG14 elevates the expression of pro-inflammatory factors (such as TNF-α and nitric oxide), thereby aggravating neuron damage by regulating miR-145-5p/PLA2G4A. 26 Therefore, these previous findings suggest that lncRNAs might be regulators in inflammation or biomarkers for disease progression in AIS.…”

Section: Discussionmentioning

confidence: 98%

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The correlation of long non‐coding RNA intersectin 1‐2 with disease risk, disease severity, inflammation, and prognosis of acute ischemic stroke

Zhang

Niu

2019

Clinical Laboratory Analysis

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Background This study aimed to evaluate the predictive value of long non‐coding RNA intersectin 1‐2 (lnc‐ITSN1‐2) for acute ischemic stroke (AIS) risk, and investigate its correlation with disease severity, inflammation, and recurrence‐free survival (RFS) in AIS patients. Methods Three hundred and twenty AIS patients were recruited, and plasma samples were collected within 24 hours after admission. lnc‐ITSN1‐2 expression form plasma was detected by reverse transcription‐quantitative polymerase chain reaction (RT‐qPCR). The National Institute of Health Stroke Scale (NIHSS) score was assessed, and RFS was calculated. Meanwhile, 320 controls were enrolled and plasma samples were collected on the enrollment, and lnc‐ITSN1‐2 expression was detected by RT‐qPCR. Results lnc‐ITSN1‐2 expression was increased in AIS patients compared to controls (P < .001), and receiver operating characteristic curve revealed its predictive value for AIS risk (area under the curve: 0.804, 95% confidence interval, 0.763‐0.845). In AIS patients, lnc‐ITSN1‐2 expression was positively correlated with NIHSS score (r = 0.464, P < .001). For inflammation, lnc‐ITSN1‐2 expression was positively correlated with CRP (r = 0.398, P < .001), TNF‐α (r = 0.502, P < .001), IL‐1β (r = 0.313, P < .001), IL‐6 (r = 0.207, P < .001), IL‐8 (r = 0.400, P < .001), IL‐17 (r = 0.272, P < .001), and IL‐22 (r = 0.222, P < .001). In terms of predicted target microRNAs, lnc‐ITSN1‐2 expression was negatively correlated with microRNA (miR)‐107 (r = −0.467, P < .001), miR‐125a (r = −0.494, P < .001), and miR‐146a (r = −0.126, P = .025). For prognosis, high lnc‐ITSN1‐2 expression was correlated with worse RFS in AIS patients. Conclusion lnc‐ITSN1‐2 exerts a good predictive value for AIS risk; meanwhile, its increased expression is correlated with enhanced disease severity, elevated inflammation, and worse RFS in AIS patients.

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Section: Discussionmentioning

confidence: 84%

Section: Discussionmentioning

confidence: 98%

The correlation of long non‐coding RNA intersectin 1‐2 with disease risk, disease severity, inflammation, and prognosis of acute ischemic stroke

Zhang

Niu

2019

Clinical Laboratory Analysis

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“…In addition to miRNAs, lncRNAs, which are a type of ncRNAs that exceed 200 nucleotides in length, have also emerged as potential factors contributing to the regulation of microglia activation states. lncRNAs H19, MALAT1, Gm4419, and SNHG14 have been reported to stimulate the activation of microglia toward the M1 phenotype and thereby promote neuroinflammation ( Qi et al, 2017 ; Wang et al, 2017 ; Wen et al, 2017 ; Han et al, 2018 ; Zhou H.J. et al, 2018 ), lncRNAs GAS5 has an inhibitory effect on M2 polarization of microglia and increases demyelination ( Sun et al, 2017 ).…”

Section: Microrna and Long Non-coding Rnamentioning

confidence: 99%

Epigenetics Control Microglia Plasticity

Cheray

Joseph

2018

Front. Cell. Neurosci.

116

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Microglia, resident immune cells of the central nervous system, fulfill multiple functions in the brain throughout life. These microglial functions range from participation in innate and adaptive immune responses, involvement in the development of the brain and its homeostasis maintenance, to contribution to degenerative, traumatic, and proliferative diseases; and take place in the developing, the aging, the healthy, or the diseased brain. Thus, an impressive level of cellular plasticity, appears as a requirement for the pleiotropic biological functions of microglia. Epigenetic changes, including histone modifications or DNA methylation as well as microRNA expression, are important modifiers of gene expression, and have been involved in cell phenotype regulation and reprogramming and are therefore part of the mechanisms regulating cellular plasticity. Here, we review and discuss the epigenetic mechanisms, which are emerging as contributors to this microglial cellular plasticity and thereby can constitute interesting targets to modulate microglia associated brain diseases, including developmental diseases, neurodegenerative diseases as well as cancer.

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“…In previous studies, OGD was shown to activate the nuclear factor (NF)-jB-dependent signaling pathway [26]. We analyzed the levels of p65, a major subunit of NF-jB, which was significantly increased in OGD cells compared with normal cultured cells and was inhibited in the lncRNA Oprm1 group ( Figure 6(D)).…”

Section: Gata3 Is a Target Of Mir-155mentioning

confidence: 92%

Overexpression of the long non-coding RNA Oprm1 alleviates apoptosis from cerebral ischemia-reperfusion injury through the Oprm1/miR-155/GATA3 axis

Jing

Liu

Jia

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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Numerous differentially expressed long non-coding RNAs (lncRNAs) have been identified in cerebral ischemia-reperfusion (I/R) injury using RNA-Seq analysis. However, little is known about whether and how lncRNAs are involved in cerebral I/R injury. In this study, we investigated the function of the lncRNA Oprm1 in cerebral I/R injury and explored the underlying mechanism. An oxygen-glucose deprivation model in N2a cells was utilized to mimic cerebral I/R injury in vitro. Trypan blue staining, terminal deoxytransferase-mediated dUTP-biotin nick end labelling and caspase-3 were measured to evaluate apoptosis. Middle cerebral artery occlusion was performed in mice to evaluate the function of lncRNA Oprm1 in vivo. Real-time PCR and western blotting were used to measure the expression levels of lncRNA Opmr1, caspase-3, miR-155, GATA binding protein 3 (GATA3) and nuclear factor (NF)-jB. lncRNA Oprm1 was mainly located in the cytoplasm. Overexpression of lncRNA Oprm1 alleviated the apoptosis induced by oxygen-glucose deprivation and significantly reduced cleaved caspase-3 levels. Infarct size was distinctly decreased in the lncRNA Oprm1-overexpression group. The neurological score was also improved. Our findings showed that the lncRNA Oprm1/miR-155/GATA3 axis plays an important role in cerebral I/R injury. lncRNA Oprm1 may attenuate cerebral injury through the NF-jB pathway. lncRNA Oprm1 may serve as a potential target for new therapeutic interventions in patients with ischemic stroke. ARTICLE HISTORY

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LncRNA Gm4419 contributes to OGD/R injury of cerebral microglial cells via IκB phosphorylation and NF-κB activation

Cited by 67 publications

References 22 publications

The correlation of long non‐coding RNA intersectin 1‐2 with disease risk, disease severity, inflammation, and prognosis of acute ischemic stroke

The correlation of long non‐coding RNA intersectin 1‐2 with disease risk, disease severity, inflammation, and prognosis of acute ischemic stroke

Epigenetics Control Microglia Plasticity

Overexpression of the long non-coding RNA Oprm1 alleviates apoptosis from cerebral ischemia-reperfusion injury through the Oprm1/miR-155/GATA3 axis

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