lncRNA GPRC5D-AS1 as a ceRNA inhibits skeletal muscle aging by regulating miR-520d-5p

Abstract

Sarcopenia induced by muscle aging is associated with negative outcomes in a variety of diseases. Long noncoding RNAs are a class of RNAs longer than 200 nucleotides with lower protein coding potential. An increasing number of studies have shown that lncRNAs play a vital role in skeletal muscle development. According to our previous research, lncRNA GPRC5D-AS1 is selected in the present study as the target gene to further study its effect on skeletal muscle aging in a dexamethasone-induced human muscle atrophy cell model. As a result, GPRC5D-AS1 functions as a ceRNA of miR-520d-5p to repress cell apoptosis and regulate the expression of muscle regulatory factors, including MyoD, MyoG, Mef2c and Myf5, thus accelerating myoblast proliferation and differentiation, facilitating development of skeletal muscle. In conclusion, lncRNA GPRC5D-AS1 could be a novel therapeutic target for treating sarcopenia.

Studying relative RNA localization From nucleus to the cytosol

“…While most of the strong cytosolic transcripts are protein coding (270, 78%), we also found 14 lncRNAs, and 23 transcripts annotated as “nonsense mediated decay”. Interestingly, many of those 14 lncRNAs were found in the literature to be associated with cytosolic localization and microRNA regulation (Han et al 2019; Tang et al 2020; YX Chen et al 2021; Zheng et al 2021; Rosano et al 2022; Yu et al 2023). Moreover, while most of the strongly nuclear transcripts are retained intron annotated transcripts (64 retained intron annotated, 2 lncRNA, 3 nonsense mediated decay), we found 10 protein coding transcripts that are strongly nuclear.…”

Role of MicroRNAs and Retroelements in the Pathogenesis of Atherosclerosis

“…Определена роль снижения экспрессии miR-493 при старении миокарда [76] Повышенная экспрессия miR-495 способствует апоптозу клеток и старению мезенхимальных стволовых клеток посредством воздействия на ген BM1 (кодирует протоонкоген BMI1) [77]. Было выявлено, что miR-520d снижает экспрессию длинной нкРНК GPRC5D-AS1, которая подавляет апоптоз клеток и активирует регуляторные факторы мышц Mef2c, Myf5, MyoD, Myo G. MiR-520d способствует старению скелетной мускулатуры [78]. Одним из признаков старения кожи является нарушение градиента кальция.…”