LncRNA HIF1A-AS2 promotes osteosarcoma progression by acting as a sponge of miR-129-5p

Abstract

Increasing studies have demonstrated that long noncoding RNAs (lncRNAs) play vital roles in tumor development and progression. However, the relationship between osteosarcoma and HIF1AAS2 remains unknown. The expression of HIF1AAS2 and miR-129-5p was detected in osteosarcoma cell lines and samples via qRT-PCR. Cell Counting Kit-8 (CCK-8) and invasion assays were performed to determine cell proliferation and invasion ability, and a dual luciferase reporter assay was performed to determine the interaction between HIF1AAS2 and miR-129-5p. We showed that the expression of HIF1A-AS2 was upregulated in the osteosarcoma samples compared with the expression in noncancerous samples. Moreover, patients with high HIF1A-AS2 expression had a shorter overall survival. Ectopic expression of HIF1A-AS2 enhanced osteosarcoma cell proliferation, cell cycle progression and invasion. We found that overexpression of miR-129-5p decreased the luciferase activity of wild-type (WT) HIF1A-AS2 but not mutant HIF1A-AS2. Ectopic expression of HIF1A-AS2 suppressed miR-129-5p expression in MG-63 cells. We demonstrated that miR-129-5p was downregulated in osteosarcoma and was negatively associated with HIF1A-AS2 expression. Furthermore, ectopic expression of miR-129-5p suppressed osteosarcoma cell proliferation, cell cycle progression and invasion. In addition, overexpression of HIF1A-AS2 promoted cell proliferation, cell cycle progression and invasion of osteosarcoma cells through the modulation of miR-129-5p. These results indicated that HIF1A-AS2 might be a potential therapeutic target for osteosarcoma.

<p>Long Noncoding RNA HIF1A-AS2 Promotes Non-Small Cell Lung Cancer Progression by the miR-153-5p/S100A14 Axis</p>

“…21 HIF1A-AS2 was also found to sponge several miRNAs, such as miR-129-5p, miR-548c-3p and miR-665. 12,22,23 In our study, we demonstrated that HIF1A-AS2 interacted with miR-153-5p. We showed that miR-153-5p expression was upregulated after HIF1A-AS2 knockdown, and HIF1A-AS2 level was negatively correlated with miR-153-5p expression in NSCLC tissues.…”

“…19 HIF1A-AS2 has been reported to participate in several cancers, such as osteosarcoma, bladder cancer, breast cancer and colorectal cancer. [12][13][14][15] Its function in NSCLC has yet to be described. Loss-of-function assays indicated that HIF1A-AS2 knockdown suppressed proliferation, migration and invasion while inducing apoptosis.…”

“…11 HIF1A-AS2 expression is positively correlated with the development of osteosarcoma, bladder cancer, breast cancer and colorectal cancer. [12][13][14][15] The roles of HIF1A-AS2 in NSCLC have not been discovered. Our current study has shown that HIF1A-AS2 expression was upregulated in NSCLC tissues and its downregulation attenuated proliferation, migration and invasion while promoting apoptosis.…”

MAFG-driven osteosarcoma cell progression is inhibited by a novel miRNA miR-4660

“… 18 , 19 , 20 miRNAs bind complementary target mRNAs, resulting in mRNA translational inhibition and/or degradation. 21 miRNA dysregulation is detected in human OS, 22 , 23 , 24 associated with OS tumorigenesis, progression, and therapy resistance. 25 , 26 Here we identify a MAFG-targeting miRNA: microRNA-4660 (miR-4660).…”

The therapeutic value of the SphK1-targeting microRNA-3677 in human osteosarcoma cells

“…MiRNAs directly bind to the 3' untranslated region (3'-UTR) of the complementary mRNAs, thereby inhibiting mRNA translation and/or inducing degradation of targeted mRNAs [8,9]. Dysregulation of miRNAs is commonly detected in human OS [10][11][12], associated with OS tumorigenesis, pathogenesis and progression [8,9].…”