LncRNA <i>NEAT1</i> Recruits SFPQ to Regulate MITF Splicing and Control RPE Cell Proliferation

Hu, Xinming; Li, Fang; He, Junhao; Yang, Juan; Jiang, Ye; Jiang, Mingyuan; Wei, Dandan; Chang, Lifu; Hejtmancik, J. Fielding; Hou, Ling; Ma, Xiaoyin

doi:10.1167/iovs.62.14.18

Cited by 14 publications

(8 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As a splicing factor in the network that we constructed, SFPQ can be recruited by the lncRNA NEAT1 and then bind directly with the mRNA of MITF to regulate its AS. SFPQ overexpression in ARPE-19 cells enhances the binding affinity of SFPQ to MITF and the splicing efficiency of ( +) MITF 17 . The results of the present study indicated that SFPQ can regulate PDR progression by influencing the AS of CCAR1.…”

Section: Discussionmentioning

confidence: 98%

“…CUGBP Elav-like family member 1 mediates fiber cell morphology by regulating the mRNA expression of the F-actin crosslinking factor Actn2 and the AS of the membrane-organization factor beta-spectrin, suggesting that post-transcriptional regulatory RBPs have evolved conserved functions to influence vertebrate oculogenesis 16 . Splicing factor proline/glutamine rich (SFPQ) binds to the mRNA of microphthalmia-associated transcription factor (MITF) and regulates retinal pigment epithelium cell proliferation by modulating the AS of MITF 17 . The RBP embryonic lethal abnormal vision like 1 regulates the AS of the eukaryotic translation initiation factor 4E nuclear import factor 1 to promote postnatal angiogenesis 18 .…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

RNA-binding proteins potentially regulate the alternative splicing of cell cycle-associated genes in proliferative diabetic retinopathy

Yang,

Zhang,

et al. 2024

Sci Rep

View full text Add to dashboard Cite

RNA-binding proteins (RBPs) contribute to the pathogenesis of proliferative diabetic retinopathy (PDR) by regulating gene expression through alternative splicing events (ASEs). However, the RBPs differentially expressed in PDR and the underlying mechanisms remain unclear. Thus, this study aimed to identify the differentially expressed genes in the neovascular membranes (NVM) and retinas of patients with PDR. The public transcriptome dataset GSE102485 was downloaded from the Gene Expression Omnibus database, and samples of PDR and normal retinas were analyzed. A mouse model of oxygen-induced retinopathy was used to confirm the results. The top 20 RBPs were screened for co-expression with alternative splicing genes (ASGs). A total of 403 RBPs were abnormally expressed in the NVM and retina samples. Functional analysis demonstrated that the ASGs were enriched in cell cycle pathways. Cell cycle-associated ASEs and an RBP–AS regulatory network, including 15 RBPs and their regulated ASGs, were extracted. Splicing factor proline/glutamine rich (SFPQ), microtubule-associated protein 1 B (MAP1B), heat-shock protein 90-alpha (HSP90AA1), microtubule-actin crosslinking factor 1 (MACF1), and CyclinH (CCNH) expression remarkably differed in the mouse model. This study provides novel insights into the RBP–AS interaction network in PDR and for developing screening and treatment options to prevent diabetic retinopathy-related blindness.

show abstract

Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

RNA-binding proteins potentially regulate the alternative splicing of cell cycle-associated genes in proliferative diabetic retinopathy

Yang,

Zhang,

et al. 2024

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Furthermore, lncRNAs can function as scaffold-like structures linking two proteins to perform a function. They can also form complementary duplexes with transcripts of protein-coding genes, interfering with mRNA splicing to produce different splice variants [ 120 ]. Moreover, they can alter the activity or cytoplasmic localization of a specific protein by binding to that protein.…”

Section: Discussionmentioning

confidence: 99%

Research Progress on Non-coding RNAs in Cholesteatoma of the Middle Ear

Liu¹,

Zhang²,

Ma³

et al. 2023

Clin Exp Otorhinolaryngol

View full text Add to dashboard Cite

Cholesteatoma of the middle ear is a common disease in otolaryngology that is receiving increasing attention. It is estimated that over 5 million people around the world are suffering from middle ear cholesteatoma. The yearly frequency of middle ear cholesteatoma is detailed as 9.2 per 100,000 in adults and 3 per 100,000 in children. Without timely discovery and intervention, cholesteatomas can become perilously large and attack intratemporal structures, coming about in various intra- and extracranial complications. So far, no practical nonsurgical treatments are available. Although multiple hypotheses exist, research directions are relatively consistent, focusing on cell proliferation, apoptosis, and bone destruction. Recently, non-coding RNA (ncRNA) especially miRNAs, lncRNAs, and circRNAs, have received increasing attention because of their key roles in gene expression, cell cycle regulation, and the development of many diseases. Although ncRNAs are not involved in protein translation, they are abundant in the genome, with only approximately 2% of genes encoding proteins and the remaining approximately 98% encoding ncRNA. The purpose of this review is to summarize the current state of knowledge regarding the specific role of ncRNA in middle ear cholesteatoma.

show abstract

“…Microphthalmia-associated transcription factor (MITF) is a key transcription factor in RPE cells. The NEAT1-splicing factor prolineand glutamine-rich (SFPQ)-MITF axis plays a critical role in RPE cell proliferation [201].…”

Section: Non-coding Rnas and Amdmentioning

confidence: 99%

Construction of an Exudative Age-Related Macular Degeneration Diagnostic and Therapeutic Molecular Network Using Multi-Layer Network Analysis, a Fuzzy Logic Model, and Deep Learning Techniques: Are Retinal and Brain Neurodegenerative Disorders Related?

Latifi-Navid,

Barzegar Behrooz,

Jamehdor

et al. 2023

Pharmaceuticals

View full text Add to dashboard Cite

Neovascular age-related macular degeneration (nAMD) is a leading cause of irreversible visual impairment in the elderly. The current management of nAMD is limited and involves regular intravitreal administration of anti-vascular endothelial growth factor (anti-VEGF). However, the effectiveness of these treatments is limited by overlapping and compensatory pathways leading to unresponsiveness to anti-VEGF treatments in a significant portion of nAMD patients. Therefore, a system view of pathways involved in pathophysiology of nAMD will have significant clinical value. The aim of this study was to identify proteins, miRNAs, long non-coding RNAs (lncRNAs), various metabolites, and single-nucleotide polymorphisms (SNPs) with a significant role in the pathogenesis of nAMD. To accomplish this goal, we conducted a multi-layer network analysis, which identified 30 key genes, six miRNAs, and four lncRNAs. We also found three key metabolites that are common with AMD, Alzheimer’s disease (AD) and schizophrenia. Moreover, we identified nine key SNPs and their related genes that are common among AMD, AD, schizophrenia, multiple sclerosis (MS), and Parkinson’s disease (PD). Thus, our findings suggest that there exists a connection between nAMD and the aforementioned neurodegenerative disorders. In addition, our study also demonstrates the effectiveness of using artificial intelligence, specifically the LSTM network, a fuzzy logic model, and genetic algorithms, to identify important metabolites in complex metabolic pathways to open new avenues for the design and/or repurposing of drugs for nAMD treatment.

show abstract

LncRNA NEAT1 Recruits SFPQ to Regulate MITF Splicing and Control RPE Cell Proliferation

Cited by 14 publications

References 59 publications

RNA-binding proteins potentially regulate the alternative splicing of cell cycle-associated genes in proliferative diabetic retinopathy

RNA-binding proteins potentially regulate the alternative splicing of cell cycle-associated genes in proliferative diabetic retinopathy

Research Progress on Non-coding RNAs in Cholesteatoma of the Middle Ear

Construction of an Exudative Age-Related Macular Degeneration Diagnostic and Therapeutic Molecular Network Using Multi-Layer Network Analysis, a Fuzzy Logic Model, and Deep Learning Techniques: Are Retinal and Brain Neurodegenerative Disorders Related?

Contact Info

Product

Resources

About